Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PWW29_RS00530 Genome accession   NZ_CP118582
Coordinates   89504..91255 (-) Length   583 a.a.
NCBI ID   WP_274883815.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 84504..96255
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW29_RS00510 - 85199..86038 (-) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  PWW29_RS00515 - 86108..87610 (-) 1503 WP_005451510.1 AAA family ATPase -
  PWW29_RS00520 aroB 87664..88764 (-) 1101 WP_038898114.1 3-dehydroquinate synthase -
  PWW29_RS00525 aroK 88796..89314 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW29_RS00530 pilQ 89504..91255 (-) 1752 WP_274883815.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW29_RS00535 pilP 91290..91805 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW29_RS00540 pilO 91795..92388 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW29_RS00545 pilN 92381..92956 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW29_RS00550 pilM 92940..93956 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63780.84 Da        Isoelectric Point: 4.5053

>NTDB_id=793595 PWW29_RS00530 WP_274883815.1 89504..91255(-) (pilQ) [Vibrio harveyi strain fish4]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSIINSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=793595 PWW29_RS00530 WP_274883815.1 89504..91255(-) (pilQ) [Vibrio harveyi strain fish4]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGACGTTACC
TTGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAACTTTCTTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGTGAAATCATTGAATCATTAGATATTCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATCGAGAACAACCTTGCAACTATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGCCCGAAT
GCTACCAGTATTGCTTTCCAAGTTGCGAAACTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCGTTGCAACAAGA
GTCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCAGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTATAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGTCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTTACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(423-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

96.913

100

0.969
  pilQ Vibrio cholerae strain A1552

77.329

97.599

0.755
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.329

97.599

0.755
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.34

0.389
  pilQ Legionella pneumophila strain Lp02

41.273

94.34

0.389
  pilQ Acinetobacter baumannii D1279779

36.833

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.833

100

0.379
  comQ Acinetobacter baylyi ADP1

36.986

100

0.37