Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PWW32_RS17245 Genome accession   NZ_CP118580
Coordinates   3645727..3646833 (-) Length   368 a.a.
NCBI ID   WP_017817653.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish3     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3640727..3651833
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW32_RS17215 - 3641312..3641809 (+) 498 WP_009697583.1 SprT family zinc-dependent metalloprotease -
  PWW32_RS17220 endA 3641961..3642656 (+) 696 WP_009705546.1 deoxyribonuclease I -
  PWW32_RS17225 rsmE 3642803..3643534 (+) 732 WP_005449742.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PWW32_RS17230 gshB 3643549..3644499 (+) 951 WP_005449741.1 glutathione synthase -
  PWW32_RS17235 - 3644629..3645192 (+) 564 WP_005449740.1 YqgE/AlgH family protein -
  PWW32_RS17240 ruvX 3645242..3645667 (+) 426 WP_005449738.1 Holliday junction resolvase RuvX -
  PWW32_RS17245 pilU 3645727..3646833 (-) 1107 WP_017817653.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWW32_RS17250 pilT 3646865..3647905 (-) 1041 WP_005425810.1 type IV pilus twitching motility protein PilT Machinery gene
  PWW32_RS17255 - 3647934..3648644 (+) 711 WP_009697582.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWW32_RS17260 proC 3648776..3649594 (+) 819 WP_199450822.1 pyrroline-5-carboxylate reductase -
  PWW32_RS17265 - 3649648..3650205 (+) 558 WP_005425806.1 YggT family protein -
  PWW32_RS17270 yggU 3650205..3650495 (+) 291 WP_005425805.1 DUF167 family protein YggU -
  PWW32_RS17275 - 3650555..3650986 (+) 432 WP_005425804.1 DUF4426 domain-containing protein -
  PWW32_RS17280 - 3651092..3651694 (+) 603 WP_274882281.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41277.40 Da        Isoelectric Point: 6.7730

>NTDB_id=793587 PWW32_RS17245 WP_017817653.1 3645727..3646833(-) (pilU) [Vibrio harveyi strain fish3]
MDLNKFLEGMLALKASDLYITVGAPILFRVDGELRPQGDKLSENDVALLLDSAMDPERRQEFRKSRESNFAIVRDCGRFR
VSAFFQRELPGAVIRRIETNIPTFEQLKLPLVLQDLAIAKRGLVLVVGATGSGKSTTMAAMTGYRNNNKTGHILTVEDPI
EFVHEHKRCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKDQKDQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEILLNSPRVSDLIRRGDLHELKATMARSNEFGMLTFDQSLY
KLVMQGKISEEDALHSADSANDLRLMLKTQRGEAFSTGSLANVKIDMD

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=793587 PWW32_RS17245 WP_017817653.1 3645727..3646833(-) (pilU) [Vibrio harveyi strain fish3]
ATGGATCTGAATAAATTTCTCGAAGGCATGCTGGCGTTAAAAGCGTCGGATCTATACATCACTGTCGGTGCCCCGATCTT
GTTTCGTGTGGATGGCGAATTGCGTCCGCAAGGCGATAAGCTGAGCGAAAATGACGTAGCCTTGTTGCTTGATAGTGCGA
TGGACCCGGAGCGACGACAGGAGTTTCGCAAAAGCCGCGAGTCGAACTTTGCCATTGTCCGTGATTGCGGTCGCTTCCGT
GTCAGTGCCTTCTTCCAACGTGAATTACCGGGAGCCGTCATTCGTCGTATTGAAACCAATATTCCAACGTTTGAGCAGCT
TAAGTTGCCTTTGGTGCTGCAAGATCTTGCAATTGCCAAGCGTGGTTTGGTGCTGGTGGTTGGTGCGACAGGCTCTGGTA
AGTCGACCACCATGGCTGCGATGACGGGTTATCGCAACAACAACAAGACTGGTCATATTCTTACGGTAGAAGACCCAATT
GAATTTGTGCATGAGCATAAACGTTGTATTGTGACTCAGCGTGAAGTGGGCTTGGATACTGACAGCTATGAAGTGGCGTT
GAAGAACTCGTTGCGTCAGGCACCAGACATGATTTTGATTGGTGAGATCCGAAGCCGAGAAACGATGGAATACGCAATGA
CCTTTGCTGAAACTGGTCACTTGTGCATGGCTACATTGCACGCCAACAACGCAAACCAAGCACTTGAACGTATTTTGCAC
TTGGTGCCGAAAGACCAGAAAGATCAGTTCTTGTTTGATCTGTCGATGAACTTAAAAGGCGTAGTAGGTCAGCAGTTGAT
CCGTGATAAGAACGGTCAGGGGCGTCATGGCGTGTTTGAGATCTTATTAAATAGCCCGCGTGTATCCGATCTGATCCGTC
GAGGCGATCTTCATGAGTTGAAAGCAACCATGGCTCGATCCAATGAATTTGGGATGTTGACCTTCGACCAATCGCTCTAC
AAGTTAGTGATGCAAGGTAAGATCAGTGAAGAAGATGCATTGCACAGTGCGGACTCTGCCAACGATCTGCGTTTGATGTT
GAAGACGCAACGTGGTGAAGCATTCTCTACGGGCAGTTTGGCGAACGTTAAGATCGATATGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(61-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

82.337

100

0.823
  pilU Pseudomonas stutzeri DSM 10701

59.143

95.109

0.563
  pilU Acinetobacter baylyi ADP1

55.556

95.38

0.53
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.308

88.315

0.391
  pilT Acinetobacter baylyi ADP1

41.57

93.478

0.389
  pilT Legionella pneumophila strain ERS1305867

41.888

92.12

0.386
  pilT Acinetobacter baumannii D1279779

41.888

92.12

0.386
  pilT Acinetobacter baumannii strain A118

41.888

92.12

0.386
  pilT Acinetobacter nosocomialis M2

41.888

92.12

0.386
  pilT Legionella pneumophila strain Lp02

41.888

92.12

0.386
  pilT Pseudomonas aeruginosa PAK

41.593

92.12

0.383
  pilT Pseudomonas stutzeri DSM 10701

40.643

92.935

0.378
  pilT Vibrio cholerae strain A1552

40.719

90.761

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.719

90.761

0.37