Detailed information NTG0793544

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: Q7586_RS24160
• Genome accession: NZ_CP131791
• Coordinates: 5165187..5166251 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain 2014S05-249
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5160187..5171251

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q7586_RS24125 (Q7586_24135)	-	5160196..5160426 (+)	231	WP_003094285.1	hypothetical protein	-
Q7586_RS24130 (Q7586_24140)	-	5160423..5160653 (+)	231	WP_023443257.1	hypothetical protein	-
Q7586_RS24135 (Q7586_24145)	-	5160640..5162850 (+)	2211	WP_031636308.1	bacteriophage protein	-
Q7586_RS24140 (Q7586_24150)	-	5162847..5163995 (+)	1149	WP_031636306.1	hypothetical protein	-
Q7586_RS24145 (Q7586_24155)	-	5163992..5164273 (+)	282	WP_126517318.1	hypothetical protein	-
Q7586_RS24150 (Q7586_24160)	-	5164347..5164571 (+)	225	WP_003121473.1	hypothetical protein	-
Q7586_RS24155 (Q7586_24165)	pilN	5164654..5165187 (-)	534	WP_342161677.1	PilN domain-containing protein	Machinery gene
Q7586_RS24160 (Q7586_24170)	pilM	5165187..5166251 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
Q7586_RS24165 (Q7586_24175)	-	5166436..5168907 (+)	2472	WP_003095841.1	penicillin-binding protein 1A	-
Q7586_RS24170 (Q7586_24180)	-	5169012..5170280 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=793544 Q7586_RS24160 WP_003110888.1 5165187..5166251(-) (pilM) [Pseudomonas aeruginosa strain 2014S05-249]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=793544 Q7586_RS24160 WP_003110888.1 5165187..5166251(-) (pilM) [Pseudomonas aeruginosa strain 2014S05-249]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCTTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466