Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PWW27_RS12250 Genome accession   NZ_CP118576
Coordinates   2602927..2603943 (-) Length   338 a.a.
NCBI ID   WP_026138410.1    Uniprot ID   -
Organism   Vibrio harveyi strain fish1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2597927..2608943
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWW27_RS12225 aroK 2598783..2599301 (-) 519 WP_005451507.1 shikimate kinase AroK -
  PWW27_RS12230 pilQ 2599491..2601242 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  PWW27_RS12235 pilP 2601277..2601792 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  PWW27_RS12240 pilO 2601782..2602375 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  PWW27_RS12245 pilN 2602368..2602943 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  PWW27_RS12250 pilM 2602927..2603943 (-) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  PWW27_RS12255 - 2604122..2606692 (+) 2571 WP_017190919.1 PBP1A family penicillin-binding protein -
  PWW27_RS12260 oxyR 2606759..2607667 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  PWW27_RS12265 - 2607842..2608570 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38053.43 Da        Isoelectric Point: 6.4206

>NTDB_id=793487 PWW27_RS12250 WP_026138410.1 2602927..2603943(-) (pilM) [Vibrio harveyi strain fish1]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=793487 PWW27_RS12250 WP_026138410.1 2602927..2603943(-) (pilM) [Vibrio harveyi strain fish1]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGCGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGTAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.828

100

0.908
  pilM Vibrio cholerae strain A1552

48.83

100

0.494