Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Q7591_RS25325 Genome accession   NZ_CP131783
Coordinates   5330589..5332082 (+) Length   497 a.a.
NCBI ID   WP_023107818.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2016C02-074     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5325589..5337082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q7591_RS25295 (Q7591_25295) - 5325801..5326610 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  Q7591_RS25300 (Q7591_25300) kdsD 5327024..5328004 (+) 981 WP_172413168.1 arabinose-5-phosphate isomerase KdsD -
  Q7591_RS25305 (Q7591_25305) - 5328004..5328543 (+) 540 WP_003098849.1 HAD family hydrolase -
  Q7591_RS25310 (Q7591_25310) lptC 5328552..5329124 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  Q7591_RS25315 (Q7591_25315) lptA 5329111..5329638 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  Q7591_RS25320 (Q7591_25320) lptB 5329638..5330363 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  Q7591_RS25325 (Q7591_25325) rpoN/rpoN1 5330589..5332082 (+) 1494 WP_023107818.1 RNA polymerase factor sigma-54 Machinery gene
  Q7591_RS25330 (Q7591_25330) raiA 5332160..5332468 (+) 309 WP_003094359.1 ribosome-associated translation inhibitor RaiA -
  Q7591_RS25335 (Q7591_25335) ptsN 5332482..5332946 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Q7591_RS25340 (Q7591_25340) rapZ 5332948..5333808 (+) 861 WP_003094361.1 RNase adapter RapZ -
  Q7591_RS25345 (Q7591_25345) - 5333838..5334110 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  Q7591_RS25350 (Q7591_25350) - 5334141..5335256 (-) 1116 WP_012077216.1 tyrosine-type recombinase/integrase -
  Q7591_RS25355 (Q7591_25355) - 5335261..5335497 (-) 237 WP_012077217.1 helix-turn-helix transcriptional regulator -
  Q7591_RS25360 (Q7591_25360) - 5335592..5335822 (+) 231 WP_012077218.1 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56028.18 Da        Isoelectric Point: 4.4327

>NTDB_id=793030 Q7591_RS25325 WP_023107818.1 5330589..5332082(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2016C02-074]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTAWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQCLHPRPGSQIESSEAEYIVPDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=793030 Q7591_RS25325 WP_023107818.1 5330589..5332082(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain 2016C02-074]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAATGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTACTGCTGCAACTGCGCCAGTTGCCGTCCACCACGGCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGTGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
CCGACGTCATTGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCGCGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCATGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.654

100

0.477