Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PWK65_RS05820 Genome accession   NZ_CP118481
Coordinates   1137588..1139393 (-) Length   601 a.a.
NCBI ID   WP_002989205.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20185322     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1117346..1164384 1137588..1139393 within 0


Gene organization within MGE regions


Location: 1117346..1164384
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWK65_RS05740 (PWK65_05680) - 1117384..1118658 (-) 1275 WP_002989251.1 polysaccharide deacetylase family protein -
  PWK65_RS05745 (PWK65_05685) - 1118754..1120181 (-) 1428 WP_002989248.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  PWK65_RS05750 (PWK65_05690) ptsP 1120366..1122099 (-) 1734 WP_002989245.1 phosphoenolpyruvate--protein phosphotransferase -
  PWK65_RS05755 (PWK65_05695) - 1122104..1122367 (-) 264 WP_002984031.1 phosphocarrier protein HPr -
  PWK65_RS05760 (PWK65_05700) - 1122761..1122979 (+) 219 WP_002989242.1 redoxin NrdH -
  PWK65_RS05765 (PWK65_05705) nrdE 1122999..1125158 (+) 2160 WP_002989239.1 class 1b ribonucleoside-diphosphate reductase subunit alpha -
  PWK65_RS05770 (PWK65_05710) nrdF 1125480..1126439 (+) 960 WP_002987365.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
  PWK65_RS05775 (PWK65_05715) - 1126501..1127727 (+) 1227 WP_370659244.1 chloride channel protein -
  PWK65_RS05780 (PWK65_05720) - 1127823..1128979 (+) 1157 Protein_1093 IS3 family transposase -
  PWK65_RS05785 (PWK65_05725) - 1129073..1129768 (-) 696 WP_002989227.1 CPBP family intramembrane glutamic endopeptidase -
  PWK65_RS05790 (PWK65_05730) - 1129787..1130548 (-) 762 WP_002989223.1 DUF3169 family protein -
  PWK65_RS05795 (PWK65_05735) - 1130545..1130760 (-) 216 WP_002989220.1 helix-turn-helix transcriptional regulator -
  PWK65_RS05800 (PWK65_05740) alaS 1131120..1133738 (-) 2619 WP_002989217.1 alanine--tRNA ligase -
  PWK65_RS05805 (PWK65_05745) prsA 1134125..1135180 (-) 1056 WP_002984003.1 peptidylprolyl isomerase PrsA -
  PWK65_RS05810 (PWK65_05750) - 1135243..1135950 (-) 708 WP_002989211.1 O-methyltransferase -
  PWK65_RS05815 (PWK65_05755) - 1136016..1137212 (-) 1197 WP_002989208.1 OFA family MFS transporter -
  PWK65_RS05820 (PWK65_05760) pepF 1137588..1139393 (-) 1806 WP_002989205.1 oligoendopeptidase F Regulator
  PWK65_RS05825 (PWK65_05765) - 1139406..1140368 (-) 963 WP_002989200.1 competence protein CoiA -
  PWK65_RS05830 (PWK65_05770) - 1140663..1141379 (-) 717 WP_002989197.1 pseudouridine synthase -
  PWK65_RS05835 (PWK65_05775) nagB 1141498..1142202 (-) 705 WP_002989194.1 glucosamine-6-phosphate deaminase -
  PWK65_RS05840 (PWK65_05780) queA 1142404..1143432 (+) 1029 WP_002989192.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  PWK65_RS05845 (PWK65_05785) - 1143439..1144659 (+) 1221 WP_002989189.1 DUF3114 domain-containing protein -
  PWK65_RS05850 (PWK65_05790) - 1144773..1145363 (-) 591 WP_002989185.1 serine hydrolase -
  PWK65_RS05855 (PWK65_05795) - 1145360..1145608 (-) 249 WP_002989183.1 serine hydrolase -
  PWK65_RS05860 (PWK65_05800) - 1145664..1145819 (-) 156 WP_002989180.1 hypothetical protein -
  PWK65_RS05865 (PWK65_05805) sodA 1145986..1146591 (-) 606 WP_002983977.1 superoxide dismutase SodA -
  PWK65_RS05870 (PWK65_05810) holA 1146688..1147728 (-) 1041 WP_002989176.1 DNA polymerase III subunit delta -
  PWK65_RS05875 (PWK65_05815) - 1147799..1150042 (-) 2244 WP_002989173.1 DNA internalization-related competence protein ComEC/Rec2 -
  PWK65_RS05880 (PWK65_05820) - 1150023..1150685 (-) 663 WP_002983967.1 ComEA family DNA-binding protein -
  PWK65_RS05885 (PWK65_05825) - 1150885..1151625 (-) 741 WP_002989170.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
  PWK65_RS05890 (PWK65_05830) - 1151743..1152519 (+) 777 WP_023612510.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PWK65_RS05895 (PWK65_05835) - 1152509..1152787 (+) 279 WP_002989164.1 GIY-YIG nuclease family protein -
  PWK65_RS05900 (PWK65_05840) - 1152811..1154811 (-) 2001 WP_002994363.1 potassium transporter Kup -
  PWK65_RS05905 (PWK65_05845) - 1154938..1156557 (-) 1620 WP_002989158.1 DEAD/DEAH box helicase -
  PWK65_RS05910 (PWK65_05850) - 1156864..1158408 (-) 1545 WP_002989155.1 peptide chain release factor 3 -
  PWK65_RS05915 (PWK65_05855) - 1158656..1159351 (-) 696 WP_002989150.1 TIGR02206 family membrane protein -
  PWK65_RS05920 (PWK65_05860) murF 1159432..1160823 (-) 1392 WP_002989148.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
  PWK65_RS05925 (PWK65_05865) - 1161022..1162068 (-) 1047 WP_002989146.1 D-alanine--D-alanine ligase -
  PWK65_RS05930 (PWK65_05870) recR 1162169..1162765 (-) 597 WP_002989144.1 recombination mediator RecR -
  PWK65_RS05935 - 1162812..1162994 (-) 183 WP_011527680.1 penicillin-binding protein -
  PWK65_RS05940 (PWK65_05875) - 1163553..1164332 (-) 780 WP_002989141.1 formate/nitrite transporter family protein -

Sequence

Protein


Download         Length: 601 a.a.        Molecular weight: 69357.19 Da        Isoelectric Point: 4.7093

>NTDB_id=792674 PWK65_RS05820 WP_002989205.1 1137588..1139393(-) (pepF) [Streptococcus pyogenes strain 20185322]
MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFSGHLLDSSANLLTITDTYLELARRVEKVYVYAHMKND
QDTTVAKYQEYQAKASGLYAKFSEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSVRQAAYEAMYSTYEQFQHTYAKTLQTNVKVQ
NYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNKHLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALE
KAEKVLAVFGKDYADRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSLHSTFTRET
QPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFRGTVFRQTQFAEFEHAIHQADQKGEVLTSEY
LNQLYADLNEKYYGLSKKDNHFIQYEWARIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPL
EVIAKAGVDMTKGDYLEAAFKVFDERLTELEVLVSKGIHKS

Nucleotide


Download         Length: 1806 bp        

>NTDB_id=792674 PWK65_RS05820 WP_002989205.1 1137588..1139393(-) (pepF) [Streptococcus pyogenes strain 20185322]
ATGACTGATAATCGCAGCCATTTAGAAGAAAAATACACTTGGGATTTGAGCACTATTTTTGCAACAGATAAGGATTGGGA
AGCTGAAGTCAGTGACTTAGCTACTGAAATTGACGCGGCTAAGGATTTTTCAGGTCATTTACTAGATTCTAGCGCTAATC
TGTTGACAATTACAGATACTTATTTAGAGTTAGCTCGTCGTGTTGAGAAAGTCTATGTTTATGCTCACATGAAAAATGAC
CAAGACACTACTGTTGCCAAGTATCAAGAATACCAAGCTAAGGCTTCAGGCTTGTATGCAAAGTTTAGCGAAGTGTTTTC
ATTCTATGACCCAGAAGTTATGGCATTGAGTCAGGAAGATTACCAAGCTTTTCTTGCGGAAACACCTGATTTAAAAAGGT
ATCATCATTTCTTTGACAAATTGTTCCAGACACGTGACCATGTGCTCAGCCAGGCTGAAGAGGAATTATTAGCAGGAGCC
CAAGAAATTTTCAACGGCGCTGAAGAAACCTTTAGTATTCTTGATAACGCTGATATTGTCTTTCCAGTAGTTAAAAATGA
TAAGGGGGAAGATGTTGAATTAACCCATGGTAACTTCATTAGCCTAATGGAATCTAAGGACCGTAGCGTTCGCCAAGCTG
CTTATGAAGCCATGTATAGTACTTATGAACAGTTCCAACATACTTACGCTAAAACCTTGCAAACCAATGTGAAAGTGCAA
AACTATAAGGCGCGTGTTCATAAATACGATTCAGCACGTCAAGCTGCCATGGCTGCTAACTTTATTCCAGAAGCTGTTTA
CGACACTTTATTAGAAACGGTTAACAAACATTTACCACTCTTACACCGTTATTTGAAATTGCGTCAAGAGGTTCTTGGTT
TAGACGATTTGAAGATGTATGATGTTTACACACCATTATCTGAAACAGATCTAGCCATTGGTTATGATGAGGCTTTGGAA
AAGGCTGAGAAGGTTTTAGCTGTTTTTGGTAAAGATTACGCAGATCGTGTGCATCGTGCTTTTACCGAACGTTGGATTGA
TGTTCATGTCAATAAAGGGAAGCGTTCTGGAGCTTATTCGGGTGGTTCCTATGATACCAATGCCTTTATGCTATTAAACT
GGCAAGATACCTTAGACAATCTATACACTTTAGTTCATGAAACTGGGCACAGTTTGCATTCTACTTTCACTCGTGAAACA
CAGCCTTATGTGTATGGAGATTACAGTATTTTCTTGGCAGAAATTGCATCCACAACGAATGAAAATATTATGACAGAAGC
TCTCTTAAATGAGGTTCAAGATGAGAAAGAACGCTTTGCCATTTTAAACCATTACCTCGATGGTTTCCGTGGAACAGTTT
TCCGTCAAACCCAATTTGCTGAGTTTGAACATGCCATTCATCAAGCTGATCAAAAAGGAGAAGTGTTAACAAGTGAATAC
CTTAACCAGCTATACGCTGATTTAAATGAAAAATATTATGGTTTAAGTAAAAAAGATAATCATTTTATTCAGTATGAATG
GGCACGTATTCCTCATTTCTATTACAATTATTATGTTTACCAATATGCAACAGGTTTTGCAGCTGCAAGCTATTTAGCGG
ATAAGATTGTGCATGGCACACAAGACGATATCGATCATTACTTGGCCTACCTCAAGTCAGGAAATTCTGATTACCCACTT
GAAGTGATTGCTAAAGCAGGCGTTGATATGACAAAAGGTGATTATTTGGAAGCCGCCTTTAAAGTCTTTGACGAACGTCT
AACAGAACTTGAAGTACTCGTGTCCAAAGGCATACACAAAAGCTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(202-581)

Oligopeptidase F (Peptidase_M3_N)

(112-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

71.215

100

0.712