Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   EsVE80_RS00700 Genome accession   NZ_AP022822
Coordinates   159126..160496 (+) Length   456 a.a.
NCBI ID   WP_173102016.1    Uniprot ID   A0A679IFR3
Organism   Enterococcus saigonensis strain VE80     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 154126..165496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EsVE80_RS00675 (EsVE80_01400) - 154864..155163 (+) 300 WP_173102011.1 hypothetical protein -
  EsVE80_RS00680 (EsVE80_01410) - 155290..155607 (+) 318 WP_173102012.1 hypothetical protein -
  EsVE80_RS00685 (EsVE80_01420) - 155791..157038 (-) 1248 WP_173102013.1 glutamate-5-semialdehyde dehydrogenase -
  EsVE80_RS00690 (EsVE80_01430) proB 157276..158082 (-) 807 WP_173102014.1 glutamate 5-kinase -
  EsVE80_RS00695 (EsVE80_01440) - 158547..159059 (+) 513 WP_173102015.1 dUTP diphosphatase -
  EsVE80_RS00700 (EsVE80_01450) radA 159126..160496 (+) 1371 WP_173102016.1 DNA repair protein RadA Machinery gene
  EsVE80_RS00705 (EsVE80_01460) - 160643..161881 (+) 1239 WP_173102017.1 PIN/TRAM domain-containing protein -
  EsVE80_RS00710 (EsVE80_01470) ispF 161874..162374 (+) 501 WP_173102018.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  EsVE80_RS00715 (EsVE80_01480) gltX 162371..163828 (+) 1458 WP_173102019.1 glutamate--tRNA ligase -
  EsVE80_RS00720 (EsVE80_01490) epsC 164288..164818 (+) 531 WP_173102020.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49660.40 Da        Isoelectric Point: 8.3222

>NTDB_id=79230 EsVE80_RS00700 WP_173102016.1 159126..160496(+) (radA) [Enterococcus saigonensis strain VE80]
MAKKPKVQFECQHCGYISPKYLGKCPNCGSWNAMVEVVIQDTTDRRVRTSLTGEKTRPTKIADVVPKKEPRVKTKLAELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAESGGKVLYVSGEESAEQIKMRAERLGSIQTEFYLYAETDMHEISR
AIENLAPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKSNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEKHHTFRILRAVKNRFGSTNEIGIFEMHEDGLHEVLNPSQVFLEERLAGATGSAIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAVAVAIASSYKEKGTSPTECFIGEIGLTG
EIRRVSNIEQRVKEVQKLGFTKVYLPKNNLSGWDAPKGIHVVGVATLAETLKKVFN

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=79230 EsVE80_RS00700 WP_173102016.1 159126..160496(+) (radA) [Enterococcus saigonensis strain VE80]
GTGGCTAAAAAGCCTAAAGTCCAATTTGAATGTCAACATTGTGGCTATATCTCACCAAAATATCTCGGTAAATGTCCCAA
CTGTGGAAGTTGGAATGCGATGGTAGAAGTGGTTATTCAAGACACAACGGATCGCCGTGTACGTACTAGTTTAACTGGTG
AGAAAACGAGACCCACGAAAATCGCCGATGTTGTCCCCAAAAAAGAACCACGGGTCAAAACAAAATTAGCAGAATTAAAC
CGCGTTTTAGGTGGTGGCGTTGTCCCTGGCTCGATGGTATTAATCGGCGGGGACCCTGGTATTGGGAAATCGACCTTGCT
TTTGCAAGTTTCGCAACAATTGGCAGAATCTGGTGGGAAAGTGTTATACGTCTCTGGTGAAGAAAGCGCGGAACAAATCA
AAATGCGGGCCGAACGTTTAGGTTCCATTCAAACGGAATTTTATTTGTACGCCGAAACGGACATGCACGAAATTTCTCGC
GCCATTGAAAATCTGGCACCAGATTATGTCATTATTGACTCGATTCAAACTATGACTCAGCCAGATATTACGAGTGTTGC
CGGTAGTGTTTCACAAGTGCGTGAAACGACTGCTGAGTTGTTAAAAATTGCTAAGTCCAACGGCATCGCCATCTTTATTG
TCGGTCATGTGACAAAAGAAGGTTCCATTGCAGGACCTAGGATGTTAGAGCATATGGTGGATACGGTGTTGTATTTTGAA
GGTGAAAAACACCATACTTTCCGTATTTTACGTGCTGTAAAAAACCGTTTTGGTTCTACGAATGAAATTGGTATTTTTGA
AATGCATGAAGATGGGTTGCATGAAGTTTTAAATCCTTCGCAGGTTTTTTTGGAGGAACGTTTGGCCGGTGCAACTGGCT
CTGCTATTGTAGTTGCGATGGAGGGTAGTCGCCCAATTTTAGTTGAAATTCAAGCCCTCGTTACCCCGACGATGTTTGGT
AATGCCAAACGCACCACCACAGGACTTGATTTTAATCGGGTATCACTCATTATGGCAGTTTTGGAAAAACGCGCCGGTTT
GCTGTTACAAAATCAAGATGCCTACTTAAAGGCAGCAGGCGGTGTGAAATTAAATGAGCCCGCGATTGATTTGGCCGTTG
CAGTGGCAATTGCTTCTAGCTATAAAGAAAAAGGAACTAGTCCCACGGAATGTTTTATTGGTGAAATTGGTTTAACCGGT
GAGATTAGACGGGTTTCAAACATTGAACAGCGGGTCAAAGAAGTACAAAAATTGGGTTTTACCAAAGTTTATCTGCCCAA
AAATAATTTAAGTGGCTGGGATGCACCAAAAGGTATTCACGTTGTTGGTGTGGCAACGCTAGCAGAAACATTAAAAAAAG
TTTTTAATTAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

AAA domain (AAA_25)

(71-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A679IFR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

74.505

99.781

0.743
  radA Streptococcus pneumoniae D39

74.505

99.781

0.743
  radA Streptococcus pneumoniae R6

74.505

99.781

0.743
  radA Streptococcus pneumoniae TIGR4

74.505

99.781

0.743
  radA Streptococcus mitis NCTC 12261

74.505

99.781

0.743
  radA Streptococcus mitis SK321

74.286

99.781

0.741
  radA Bacillus subtilis subsp. subtilis str. 168

66.741

98.904

0.66

Multiple sequence alignment