Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   PVK50_RS00665 Genome accession   NZ_CP118311
Coordinates   102622..103656 (+) Length   344 a.a.
NCBI ID   WP_223845212.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20164915     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97622..108656
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK50_RS00655 (PVK50_00650) - 101290..101655 (+) 366 WP_002986560.1 DUF1033 family protein -
  PVK50_RS00660 (PVK50_00655) comYA 101748..102686 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  PVK50_RS00665 (PVK50_00660) comYB 102622..103656 (+) 1035 WP_223845212.1 competence type IV pilus assembly protein ComGB Machinery gene
  PVK50_RS00670 (PVK50_00665) comYC 103658..103984 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PVK50_RS00675 (PVK50_00670) comGD 103959..104387 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PVK50_RS00680 (PVK50_00675) comGE 104344..104628 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PVK50_RS00685 (PVK50_00680) comYF 104621..105055 (+) 435 WP_002987783.1 competence type IV pilus minor pilin ComGF Machinery gene
  PVK50_RS00690 (PVK50_00685) comGG 105039..105365 (+) 327 WP_002987787.1 competence type IV pilus minor pilin ComGG -
  PVK50_RS00695 (PVK50_00690) comYH 105463..106416 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PVK50_RS00700 (PVK50_00695) - 106475..107671 (+) 1197 WP_002987791.1 acetate kinase -
  PVK50_RS00705 (PVK50_00700) - 107858..108166 (+) 309 WP_011284425.1 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39734.23 Da        Isoelectric Point: 9.7085

>NTDB_id=791723 PVK50_RS00665 WP_223845212.1 102622..103656(+) (comYB) [Streptococcus pyogenes strain 20164915]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWSTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGVELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=791723 PVK50_RS00665 WP_223845212.1 102622..103656(+) (comYB) [Streptococcus pyogenes strain 20164915]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATGCTAGAGAATGGAGTACGTTAATAGGTCAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGTTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

42.462

94.477

0.401