Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PUW21_RS13940 Genome accession   NZ_CP118167
Coordinates   2664067..2664477 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain 21855     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2659067..2669477
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS13910 (PUW21_13910) - 2659154..2659294 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  PUW21_RS13915 (PUW21_13915) yqeF 2659600..2660331 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  PUW21_RS13920 (PUW21_13920) cwlH 2660583..2661335 (-) 753 WP_046381197.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  PUW21_RS13925 (PUW21_13925) yqeD 2661522..2662148 (+) 627 WP_046381198.1 TVP38/TMEM64 family protein -
  PUW21_RS13930 (PUW21_13930) gnd 2662167..2663060 (-) 894 WP_038429331.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PUW21_RS13935 (PUW21_13935) yqeB 2663312..2664034 (+) 723 WP_010886572.1 hypothetical protein -
  PUW21_RS13940 (PUW21_13940) nucA/comI 2664067..2664477 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  PUW21_RS13945 (PUW21_13945) sigK 2664673..2665401 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  PUW21_RS13950 (PUW21_13950) - 2665401..2665517 (+) 117 WP_122894874.1 hypothetical protein -
  PUW21_RS13955 (PUW21_13955) - 2665483..2665679 (-) 197 Protein_2701 recombinase family protein -
  PUW21_RS13960 (PUW21_13960) - 2665637..2665801 (-) 165 WP_080265384.1 hypothetical protein -
  PUW21_RS13965 (PUW21_13965) - 2666234..2666827 (-) 594 WP_029318098.1 TetR/AcrR family transcriptional regulator -
  PUW21_RS13970 (PUW21_13970) - 2666985..2667980 (+) 996 WP_029318099.1 acryloyl-CoA reductase -
  PUW21_RS13975 (PUW21_13975) - 2668308..2668541 (+) 234 Protein_2705 helix-turn-helix domain-containing protein -
  PUW21_RS13980 (PUW21_13980) arsC 2668752..2669171 (-) 420 WP_029318100.1 arsenate reductase (thioredoxin) -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=790987 PUW21_RS13940 WP_009967785.1 2664067..2664477(-) (nucA/comI) [Bacillus subtilis strain 21855]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=790987 PUW21_RS13940 WP_009967785.1 2664067..2664477(-) (nucA/comI) [Bacillus subtilis strain 21855]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529