Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PUW21_RS13415 Genome accession   NZ_CP118167
Coordinates   2570686..2571756 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain 21855     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2565686..2576756
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW21_RS13370 (PUW21_13370) tapA 2566165..2566926 (-) 762 WP_003230180.1 amyloid fiber anchoring/assembly protein TapA -
  PUW21_RS13375 (PUW21_13375) yqzG 2567198..2567524 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  PUW21_RS13380 (PUW21_13380) spoIIT 2567566..2567745 (-) 180 WP_003230176.1 YqzE family protein -
  PUW21_RS13385 (PUW21_13385) comGG 2567816..2568190 (-) 375 WP_003230170.1 ComG operon protein ComGG Machinery gene
  PUW21_RS13390 (PUW21_13390) comGF 2568191..2568574 (-) 384 WP_003230168.1 ComG operon protein ComGF Machinery gene
  PUW21_RS13395 (PUW21_13395) comGE 2568600..2568947 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  PUW21_RS13400 (PUW21_13400) comGD 2568931..2569362 (-) 432 WP_004398628.1 comG operon protein ComGD Machinery gene
  PUW21_RS13405 (PUW21_13405) comGC 2569352..2569648 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  PUW21_RS13410 (PUW21_13410) comGB 2569662..2570699 (-) 1038 WP_009967727.1 comG operon protein ComGB Machinery gene
  PUW21_RS13415 (PUW21_13415) comGA 2570686..2571756 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  PUW21_RS13420 (PUW21_13420) corA 2572168..2573121 (-) 954 WP_004399136.1 magnesium transporter CorA -
  PUW21_RS13425 (PUW21_13425) yqhB 2573264..2574592 (+) 1329 WP_009967730.1 hemolysin family protein -
  PUW21_RS13430 (PUW21_13430) rsbRD 2574645..2575481 (-) 837 WP_004398754.1 RsbT co-antagonist protein RsbRD -
  PUW21_RS13440 (PUW21_13440) mgsR 2575705..2576085 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  PUW21_RS13445 (PUW21_13445) yqgY 2576317..2576562 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=790982 PUW21_RS13415 WP_004399124.1 2570686..2571756(-) (comGA) [Bacillus subtilis strain 21855]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=790982 PUW21_RS13415 WP_004399124.1 2570686..2571756(-) (comGA) [Bacillus subtilis strain 21855]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGTTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACTAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1