Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PUP54_RS25020 Genome accession   NZ_CP118151
Coordinates   5509348..5510565 (+) Length   405 a.a.
NCBI ID   WP_053262703.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain ATCC 9446     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5504348..5515565
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUP54_RS25005 (PUP54_25005) - 5504803..5507721 (+) 2919 WP_053262702.1 DUF748 domain-containing protein -
  PUP54_RS25010 (PUP54_25010) - 5507925..5508281 (-) 357 WP_009045666.1 BON domain-containing protein -
  PUP54_RS25015 (PUP54_25015) - 5508568..5508984 (-) 417 WP_007924673.1 pilin -
  PUP54_RS25020 (PUP54_25020) pilC 5509348..5510565 (+) 1218 WP_053262703.1 type II secretion system F family protein Machinery gene
  PUP54_RS25025 (PUP54_25025) pilD 5510568..5511440 (+) 873 WP_053262704.1 A24 family peptidase Machinery gene
  PUP54_RS25030 (PUP54_25030) coaE 5511437..5512060 (+) 624 WP_009045669.1 dephospho-CoA kinase -
  PUP54_RS25035 (PUP54_25035) yacG 5512057..5512260 (+) 204 WP_009045670.1 DNA gyrase inhibitor YacG -
  PUP54_RS25040 (PUP54_25040) - 5512266..5512481 (-) 216 WP_053262705.1 hypothetical protein -
  PUP54_RS25045 (PUP54_25045) - 5512547..5513236 (-) 690 WP_053262706.1 energy-coupling factor ABC transporter permease -
  PUP54_RS25050 (PUP54_25050) - 5513233..5513700 (-) 468 WP_053262707.1 hypothetical protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44504.41 Da        Isoelectric Point: 10.4761

>NTDB_id=790705 PUP54_RS25020 WP_053262703.1 5509348..5510565(+) (pilC) [Pseudomonas chlororaphis strain ATCC 9446]
MAVKAAKVSVYLWEGTDRQGSKLRGELSGHTPSLIRAQLRKQGINPARVRKKSNSLLQRTPPIKARDISLFTRQLATLIK
AGVPLLQSFDIIAEGFDNPNMRKLMGELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVICVALVVTGILLVKVVPQFQSMFQGFGAQLPVFTQLVIALSELVQHWWWLLVGALLLSVFGLR
HARRRSPRFRHWLDVGLLKLPLVAPLMYKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQDVATGM
QLHFSMRASGIFPGMAIQMTAIGEESGALDGMLEKVAIHYEEEVDTLVDTLSSLMEPVIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=790705 PUP54_RS25020 WP_053262703.1 5509348..5510565(+) (pilC) [Pseudomonas chlororaphis strain ATCC 9446]
ATGGCGGTCAAAGCAGCAAAAGTCAGTGTTTACCTGTGGGAAGGCACCGACAGGCAAGGCAGCAAACTGCGCGGCGAATT
GAGCGGCCATACCCCCTCGCTGATCAGGGCCCAGTTGCGCAAGCAAGGCATCAACCCGGCCAGGGTCAGGAAGAAGTCCA
ACTCGCTGTTGCAGCGCACGCCCCCGATCAAGGCCCGCGACATCAGCCTGTTCACCCGGCAACTGGCGACCCTGATCAAG
GCCGGCGTACCACTGCTGCAATCGTTCGACATCATTGCCGAGGGCTTCGATAACCCGAACATGCGCAAACTGATGGGCGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCGCTGCGCAAGAGGCCGCGGTACTTCGACGAGCTGTACT
GCAGCCTGGTGGATGCCGGCGAACAGGCCGGGGCCCTGGAAACCCTACTGGAGCGGGTGGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACACGGATCCGCAAGGCCATGACTTATCCCCTGGCGGTGATCTGCGTGGCGCTGGTGGTGACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGTCGATGTTCCAGGGCTTCGGCGCGCAATTGCCGGTGTTCACGCAACTGG
TCATCGCCCTCTCGGAACTCGTGCAGCACTGGTGGTGGCTGCTGGTGGGCGCGCTGTTGCTCAGTGTGTTCGGCCTGCGC
CATGCCCGCCGGCGCTCACCACGCTTTCGCCACTGGCTGGACGTGGGCCTGCTGAAACTGCCGTTGGTAGCGCCACTGAT
GTACAAGTCCGCGGTGGCCCGCTACGCCCGCACCTTGTCCACCACTTTCGCCGCCGGAGTGCCGCTGGTGGAGGCCTTGG
GCTCGGTGGCCGGGGCCACCGGCAACCAGGTGTTCAAGCAAGCGGTGAACCGCATCAGGCAGGACGTGGCGACCGGCATG
CAGTTGCATTTTTCCATGCGCGCATCCGGCATCTTTCCCGGCATGGCCATCCAGATGACCGCCATCGGCGAAGAGTCCGG
GGCCCTGGACGGCATGCTGGAAAAGGTCGCGATCCATTATGAGGAAGAGGTCGATACCCTGGTCGACACCCTGAGCAGTC
TCATGGAACCCGTGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

65.432

100

0.654
  pilC Acinetobacter baylyi ADP1

56.127

100

0.565
  pilC Acinetobacter baumannii D1279779

55.392

100

0.558
  pilC Legionella pneumophila strain ERS1305867

52.02

97.778

0.509
  pilG Neisseria gonorrhoeae MS11

41.542

99.259

0.412
  pilC Vibrio cholerae strain A1552

42.347

96.79

0.41
  pilG Neisseria meningitidis 44/76-A

40.796

99.259

0.405
  pilC Vibrio campbellii strain DS40M4

39.055

99.259

0.388