Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PUW24_RS04640 Genome accession   NZ_CP118106
Coordinates   750018..751385 (-) Length   455 a.a.
NCBI ID   WP_047914117.1    Uniprot ID   A0A069DKD5
Organism   Paenibacillus urinalis strain 2022CK-00833     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 745018..756385
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW24_RS04615 (PUW24_04615) ispD 745475..746161 (-) 687 WP_047914112.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PUW24_RS04620 (PUW24_04620) - 746235..747323 (-) 1089 WP_047914113.1 PIN/TRAM domain-containing protein -
  PUW24_RS04625 (PUW24_04625) - 747572..747967 (+) 396 WP_047914114.1 hypothetical protein -
  PUW24_RS04630 (PUW24_04630) pssA 748143..748871 (-) 729 WP_047914115.1 CDP-diacylglycerol--serine O-phosphatidyltransferase -
  PUW24_RS04635 (PUW24_04635) disA 748928..750004 (-) 1077 WP_047914116.1 DNA integrity scanning diadenylate cyclase DisA -
  PUW24_RS04640 (PUW24_04640) radA 750018..751385 (-) 1368 WP_047914117.1 DNA repair protein RadA Machinery gene
  PUW24_RS04645 (PUW24_04645) clpC 751696..754149 (-) 2454 WP_047914118.1 ATP-dependent protease ATP-binding subunit ClpC -
  PUW24_RS04650 (PUW24_04650) - 754343..755404 (-) 1062 WP_047914119.1 protein arginine kinase -
  PUW24_RS04655 (PUW24_04655) - 755436..755957 (-) 522 WP_047914120.1 UvrB/UvrC motif-containing protein -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 49645.32 Da        Isoelectric Point: 6.8328

>NTDB_id=790199 PUW24_RS04640 WP_047914117.1 750018..751385(-) (radA) [Paenibacillus urinalis strain 2022CK-00833]
MAKVKTKFYCTECGYEAPKWYGKCPGCQSWNSMVEETESIVKTQGRGSSLFDSKEKPQPIINIESDQEPRILTGIKELNR
VLGGGVVPGSLVLVGGDPGIGKSTLLLQTSHAMTTAGLRVLYISGEESVRQTKLRADRLEALSPELYVLSETNMERIEEA
VKQVEPHFLVIDSIQTVYLPEITSAPGSVAQVRECTSRFMRIAKGQGIATVLVGHVTKEGAIAGPRMLEHMVDCVLYFEG
ERHHTYRLLRAVKNRFGSTNEMGIFEMGEIGLTEVANPSEMFLSERPLGVAGSTVAASMEGTRPMLVELQALVSATQFPS
PRRMATGVDYNRMALIIAVLEKRQGMFLQNQDAYINVAGGVKLDEPAIDLAVAVSVASSFRDMPTKPFDVVFGEVGLTGE
VRAVSRAEQRAKEAQKLGFKRVIMPEKSLKGWKHPKGIELIGVNTVGDALAVALD

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=790199 PUW24_RS04640 WP_047914117.1 750018..751385(-) (radA) [Paenibacillus urinalis strain 2022CK-00833]
GTGGCTAAAGTTAAAACGAAATTTTATTGTACTGAATGCGGCTATGAAGCCCCTAAATGGTACGGTAAATGTCCAGGATG
TCAGTCCTGGAACTCCATGGTGGAGGAAACCGAAAGTATCGTTAAGACACAAGGGAGAGGTTCTTCCCTTTTTGATAGTA
AAGAAAAGCCTCAGCCCATCATAAACATAGAGAGTGACCAGGAGCCCCGCATCCTGACGGGTATTAAAGAGCTGAATCGA
GTGCTCGGCGGCGGCGTCGTTCCGGGCTCTCTTGTTCTGGTCGGCGGAGATCCGGGCATCGGTAAATCTACATTACTGTT
GCAAACCTCCCATGCCATGACCACAGCAGGACTGCGCGTGCTGTATATTTCCGGTGAGGAATCGGTTAGGCAGACGAAGC
TGAGGGCGGATCGACTGGAAGCATTGTCTCCTGAGCTGTATGTGCTCAGCGAAACAAATATGGAACGGATTGAGGAAGCA
GTCAAACAGGTAGAGCCGCATTTCTTAGTCATTGACTCCATTCAAACGGTATATTTACCAGAAATTACGAGTGCACCGGG
CAGTGTGGCACAAGTTAGAGAATGTACATCAAGATTTATGCGAATCGCCAAAGGTCAGGGCATTGCGACCGTGCTTGTAG
GGCATGTTACTAAGGAAGGTGCTATTGCAGGACCGCGAATGCTTGAGCATATGGTGGATTGTGTGCTCTATTTTGAAGGC
GAGAGACATCATACGTACCGGCTGCTTCGTGCGGTGAAGAACCGTTTTGGCTCAACCAATGAAATGGGGATTTTTGAGAT
GGGTGAAATCGGGTTAACCGAGGTTGCCAATCCATCCGAGATGTTCTTGTCTGAACGACCGCTTGGGGTAGCAGGCTCTA
CTGTTGCTGCCAGCATGGAAGGGACCCGTCCAATGCTTGTTGAGCTTCAGGCGCTGGTATCCGCAACCCAATTCCCTTCT
CCAAGACGTATGGCTACCGGTGTAGATTACAATCGTATGGCTCTTATTATCGCCGTATTGGAAAAGCGCCAGGGTATGTT
CTTACAGAACCAGGATGCTTATATAAACGTCGCCGGAGGGGTGAAGCTGGATGAACCTGCTATTGATCTGGCGGTTGCCG
TCAGTGTCGCATCCAGCTTCCGGGATATGCCGACCAAACCTTTTGATGTTGTGTTTGGAGAGGTAGGTCTTACAGGCGAG
GTCCGGGCCGTGTCTCGTGCGGAGCAGAGAGCAAAAGAAGCGCAGAAGCTTGGATTCAAACGGGTGATTATGCCAGAGAA
GAGCCTAAAAGGCTGGAAGCACCCGAAGGGAATAGAACTTATCGGTGTAAACACCGTCGGAGATGCACTAGCGGTTGCTT
TAGATTAG

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-220)

Subunit ChlI of Mg-chelatase (ChlI)

(344-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A069DKD5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.886

100

0.67
  radA Streptococcus pneumoniae Rx1

58.667

98.901

0.58
  radA Streptococcus pneumoniae D39

58.667

98.901

0.58
  radA Streptococcus pneumoniae R6

58.667

98.901

0.58
  radA Streptococcus pneumoniae TIGR4

58.667

98.901

0.58
  radA Streptococcus mitis NCTC 12261

58.667

98.901

0.58
  radA Streptococcus mitis SK321

58.444

98.901

0.578