Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW54_RS10420 Genome accession   NZ_CP118087
Coordinates   2077449..2079245 (+) Length   598 a.a.
NCBI ID   WP_005718172.1    Uniprot ID   A0A135YP31
Organism   Lactobacillus crispatus strain VSI08     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2072449..2084245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW54_RS10390 (PUW54_10390) - 2073346..2073723 (-) 378 WP_005718180.1 hypothetical protein -
  PUW54_RS10395 (PUW54_10395) - 2073868..2074419 (+) 552 WP_005722451.1 GNAT family protein -
  PUW54_RS10400 (PUW54_10400) - 2074734..2074895 (+) 162 WP_005718177.1 hypothetical protein -
  PUW54_RS10405 (PUW54_10405) - 2074909..2075292 (+) 384 WP_005718176.1 PBECR4 domain-containing protein -
  PUW54_RS10410 (PUW54_10410) - 2075289..2075978 (-) 690 WP_005718174.1 DUF554 domain-containing protein -
  PUW54_RS10415 (PUW54_10415) - 2076043..2077281 (-) 1239 WP_240397182.1 LCP family protein -
  PUW54_RS10420 (PUW54_10420) pepF 2077449..2079245 (+) 1797 WP_005718172.1 oligoendopeptidase F Regulator
  PUW54_RS10425 (PUW54_10425) - 2079326..2079598 (-) 273 WP_005718171.1 bacteriocin immunity protein -
  PUW54_RS10430 (PUW54_10430) - 2079676..2080473 (-) 798 WP_020993066.1 CPBP family intramembrane glutamic endopeptidase -
  PUW54_RS10435 (PUW54_10435) - 2080726..2080866 (-) 141 WP_020993067.1 bacteriocin -
  PUW54_RS10440 (PUW54_10440) - 2080910..2081068 (-) 159 WP_164913815.1 hypothetical protein -
  PUW54_RS10445 (PUW54_10445) - 2081428..2081607 (-) 180 WP_005718165.1 hypothetical protein -
  PUW54_RS10450 (PUW54_10450) - 2081815..2082453 (-) 639 WP_005718164.1 HlyD family efflux transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68032.88 Da        Isoelectric Point: 4.6146

>NTDB_id=789996 PUW54_RS10420 WP_005718172.1 2077449..2079245(+) (pepF) [Lactobacillus crispatus strain VSI08]
MAIPNRNEVPEELKWDLTRIFKNDEEWEQAYAAAQEKIDQLGELKGTLAKSGKDLYEGLTKILAVKREVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLAEFKQAEPRLKNYAHYLEMITNKRPHTLPAEQEKLIAD
AGDALGVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYSQFQNSLASTLSGVVKK
HNYNAKAHKYDSARQAALAENGVPEAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPSLSYNFEEA
KEVAKKALKPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADANGEPLTA
DTLDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=789996 PUW54_RS10420 WP_005718172.1 2077449..2079245(+) (pepF) [Lactobacillus crispatus strain VSI08]
ATGGCGATACCAAACAGAAATGAAGTCCCAGAAGAACTTAAGTGGGACTTGACCCGAATTTTTAAAAATGATGAAGAATG
GGAACAGGCCTATGCAGCGGCCCAAGAAAAAATCGACCAACTTGGTGAATTAAAAGGTACATTAGCAAAATCTGGCAAAG
ATTTGTACGAAGGCTTAACTAAGATTCTGGCCGTTAAGCGCGAGGTGGAAAATATTTATGTTTATGCCACCATGTCTAGC
GATGTGGATACTGCCAATTCACATTATTTAGGCTATGTCAGCCGGGTGCAAAGCTTAGCTAACCAATTCGAAGCCGCTAC
TAGTTTTATTAACCCAGAAATCTTGAGCATCCCTAGTGATAAACTAGCTGAATTTAAGCAAGCTGAGCCAAGACTAAAGA
ATTATGCTCACTACTTGGAAATGATTACTAATAAGCGACCTCATACCCTGCCAGCTGAACAAGAAAAGTTAATCGCTGAT
GCTGGAGATGCTTTAGGCGTTTCCGAAAATACTTTTAACGTTTTAACTAACTCTGACATGGAATACGGCTACGTCCAAGA
TGACGATGGCAACATGGAACAACTATCTGACGGTTTGTACTCATTATTAATTCAATCGCAAAACCGTGACGTCAGAAAAG
GTGCCTTCGACACTCTCTATGCTACGTACAGCCAATTCCAGAATTCGCTGGCTTCAACCTTGTCCGGCGTAGTGAAAAAA
CACAACTATAACGCTAAAGCGCACAAATATGACTCTGCCCGTCAAGCAGCACTGGCTGAAAACGGCGTACCTGAAGCAGT
TTACGATACTTTAATTCAAGAAGTTGATTCTCACCTTGACCTGCTCCATCGTTACGTTGCCTTGCGCAAGAAAATTTTAG
GCTTAAAAGATTTGCAAATGTGGGACATGTACGTGCCGCTAACTGGGAAGCCTTCTCTTTCTTATAACTTTGAAGAAGCT
AAGGAAGTTGCTAAAAAGGCATTGAAGCCTTTGGGCGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAACAAGGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCCGATGCTAATGGAGAACCACTGACCGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCGATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACAGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGCTTTCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGTGCTGGCGTAGACATGACTAAGCCTGACTACTTAGAAGATGCCTTCAAGACTTTTGAAAA
ACGGTTGAACGAATTTGAAAGCTTGATTGAGAAATAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(203-583)

Oligopeptidase F (Peptidase_M3_N)

(113-181)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A135YP31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.515

99.331

0.512