Detailed information    

insolico Bioinformatically predicted

Overview

Name   amiF   Type   Regulator
Locus tag   PUW52_RS08325 Genome accession   NZ_CP118078
Coordinates   1660392..1661315 (-) Length   307 a.a.
NCBI ID   WP_024052698.1    Uniprot ID   A0A412PMR5
Organism   Streptococcus anginosus strain VSI16     
Function   internalize XIP (predicted from homology)   
Competence regulation

Genomic Context

Location: 1655392..1666315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW52_RS08305 (PUW52_08305) - 1656950..1658407 (+) 1458 WP_024052695.1 sucrose-6-phosphate hydrolase -
  PUW52_RS08310 (PUW52_08310) - 1658388..1659353 (+) 966 WP_003031743.1 LacI family DNA-binding transcriptional regulator -
  PUW52_RS10680 - 1659427..1660249 (-) 823 Protein_1591 Cof-type HAD-IIB family hydrolase -
  PUW52_RS08325 (PUW52_08325) amiF 1660392..1661315 (-) 924 WP_024052698.1 ATP-binding cassette domain-containing protein Regulator
  PUW52_RS08330 (PUW52_08330) amiE 1661326..1662393 (-) 1068 WP_003026176.1 ABC transporter ATP-binding protein Regulator
  PUW52_RS08335 (PUW52_08335) amiD 1662402..1663328 (-) 927 WP_024052699.1 oligopeptide ABC transporter permease OppC Regulator
  PUW52_RS08340 (PUW52_08340) amiC 1663328..1664824 (-) 1497 WP_024052700.1 ABC transporter permease Regulator

Sequence

Protein


Download         Length: 307 a.a.        Molecular weight: 34870.06 Da        Isoelectric Point: 6.5117

>NTDB_id=789857 PUW52_RS08325 WP_024052698.1 1660392..1661315(-) (amiF) [Streptococcus anginosus strain VSI16]
MPEKLVEVKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGESGSGKTTIGRAIIGLNDTSHGEIFFEGKKINGKKSKE
EESEIIRKIQMIFQDPAASLNERATVDYIISEGLYNFHLFKDEEERKKKVKDIIHEVGLLSEHLTRYPHEFSGGQRQRIG
IARALVMEPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFISDRIAVIYKGVIVEVAETEELFN
NPIHPYTQSLLSAVPIPDPILERKKVLKVYDANHHDYSTDKPEMVEIRPGHFVWANKAEVEKYKKEL

Nucleotide


Download         Length: 924 bp        

>NTDB_id=789857 PUW52_RS08325 WP_024052698.1 1660392..1661315(-) (amiF) [Streptococcus anginosus strain VSI16]
ATGCCTGAAAAATTAGTAGAAGTTAAAGATTTGGAAATTTCCTTCGGTGAGGGAAGCAAGAAATTTGTTGCTGTTAAAAA
TGCTAATTTCTTTATCAATAAAGGAGAAACGTTTTCACTTGTAGGAGAATCTGGTTCTGGTAAAACGACAATTGGTCGTG
CGATTATTGGTTTAAATGATACGAGTCATGGAGAGATTTTCTTTGAAGGGAAAAAAATCAATGGCAAAAAATCAAAAGAA
GAAGAGTCAGAAATCATTCGTAAAATTCAAATGATTTTCCAGGATCCTGCTGCTAGTTTGAATGAACGTGCGACAGTTGA
TTATATCATTTCGGAAGGCTTGTATAATTTCCATTTATTTAAAGATGAAGAAGAACGCAAGAAAAAAGTGAAAGATATTA
TTCATGAAGTTGGATTGTTATCAGAGCATTTAACACGTTACCCGCATGAATTTTCTGGTGGTCAACGTCAGCGGATTGGG
ATTGCCCGTGCGCTTGTTATGGAACCAGATTTTGTCATTGCAGATGAGCCGATTTCAGCTTTGGACGTGTCTGTGCGGGC
ACAAGTTTTAAACCTACTGAAAAAATTCCAAAAAGAATTAGGTTTGACTTATCTCTTTATTGCTCACGATTTATCGGTTG
TACGCTTCATTTCAGATCGTATTGCGGTTATTTATAAGGGTGTGATTGTCGAAGTAGCTGAAACAGAAGAACTCTTTAAC
AATCCAATTCATCCTTATACTCAGTCTTTATTGTCAGCTGTGCCAATTCCAGATCCAATCTTGGAACGGAAGAAAGTGTT
GAAAGTCTATGATGCTAATCATCATGATTATTCAACGGATAAGCCAGAAATGGTCGAAATTCGTCCTGGACACTTTGTGT
GGGCTAATAAAGCAGAAGTTGAAAAATATAAAAAAGAATTATAA

Domains

Predicted by InterproScan.

ABC transporter (ABC_tran)

(25-178)

Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY)

(230-262)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A412PMR5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  amiF Streptococcus thermophilus LMG 18311

87.908

99.674

0.876
  amiF Streptococcus salivarius strain HSISS4

87.908

99.674

0.876
  amiF Streptococcus thermophilus LMD-9

87.582

99.674

0.873