Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW61_RS11290 Genome accession   NZ_CP118069
Coordinates   2201850..2203646 (+) Length   598 a.a.
NCBI ID   WP_274998404.1    Uniprot ID   -
Organism   Lactobacillus crispatus strain VSI21     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2196850..2208646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW61_RS11265 (PUW61_11265) - 2197070..2198611 (-) 1542 WP_005718181.1 ABC-F family ATP-binding cassette domain-containing protein -
  PUW61_RS11270 (PUW61_11270) - 2198625..2199002 (-) 378 WP_060463891.1 hypothetical protein -
  PUW61_RS11275 (PUW61_11275) - 2199147..2199698 (+) 552 WP_060463890.1 GNAT family protein -
  PUW61_RS11280 (PUW61_11280) - 2199695..2200384 (-) 690 WP_060463889.1 DUF554 domain-containing protein -
  PUW61_RS11285 (PUW61_11285) - 2200450..2201682 (-) 1233 WP_060463888.1 LCP family protein -
  PUW61_RS11290 (PUW61_11290) pepF 2201850..2203646 (+) 1797 WP_274998404.1 oligoendopeptidase F Regulator
  PUW61_RS11295 (PUW61_11295) - 2203727..2203999 (-) 273 WP_005718171.1 bacteriocin immunity protein -
  PUW61_RS11300 (PUW61_11300) - 2204077..2204874 (-) 798 WP_020993066.1 CPBP family intramembrane glutamic endopeptidase -
  PUW61_RS11305 (PUW61_11305) - 2205127..2205267 (-) 141 WP_020993067.1 bacteriocin -
  PUW61_RS11310 (PUW61_11310) - 2205311..2205469 (-) 159 WP_164913815.1 hypothetical protein -
  PUW61_RS11315 (PUW61_11315) - 2205829..2206008 (-) 180 WP_005718165.1 hypothetical protein -
  PUW61_RS11320 (PUW61_11320) - 2206216..2206854 (-) 639 WP_005718164.1 HlyD family efflux transporter periplasmic adaptor subunit -

Sequence

Protein


Download         Length: 598 a.a.        Molecular weight: 68066.90 Da        Isoelectric Point: 4.6146

>NTDB_id=789782 PUW61_RS11290 WP_274998404.1 2201850..2203646(+) (pepF) [Lactobacillus crispatus strain VSI21]
MAIPNRNEVPEELKWDLTRIFKNDEEWEQAYAAAQEKIDQLGELKGTLAKSGKDFYEGLTKILAVKREVENIYVYATMSS
DVDTANSHYLGYVSRVQSLANQFEAATSFINPEILSIPSDKLAEFKQAEPRLKNYAHYLEMITNKRPHTLPAEQEKLIAD
AGDALGVSENTFNVLTNSDMEYGYVQDDDGNMEQLSDGLYSLLIQSQNRDVRKGAFDTLYATYSQFQNSLASTLSGVVKK
HNYNAKAHKYDSARQAALAENGVPEAVYDTLIQEVDSHLDLLHRYVALRKKILGLKDLQMWDMYVPLTGKPSLSYNFEEA
KEVAKKALKPLGEDYLKHVDYIFNNRVIDPIESKNKVTGAYSGGAYDTDPYELLNWEDNIDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDHITDPKTRAFILNYYLDSFKGTLFRQTQFAVFEQFLHEADANGEPLTA
DTLDDVYGQINQHYYGDSVEPGGDIALEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTPAERDAYLGFLKAGSSDY
PTEIMKRAGVDMTKPDYLEDAFKTFEKRLNEFESLIEK

Nucleotide


Download         Length: 1797 bp        

>NTDB_id=789782 PUW61_RS11290 WP_274998404.1 2201850..2203646(+) (pepF) [Lactobacillus crispatus strain VSI21]
ATGGCGATACCAAACAGAAATGAAGTCCCAGAAGAACTTAAGTGGGACTTGACCCGAATTTTTAAAAATGATGAAGAATG
GGAACAGGCCTATGCAGCGGCCCAAGAAAAAATCGACCAACTTGGTGAATTAAAAGGTACATTAGCAAAATCTGGCAAAG
ATTTCTACGAAGGCTTAACTAAGATTCTGGCCGTTAAGCGCGAGGTGGAAAATATTTATGTTTATGCCACCATGTCTAGC
GATGTGGATACTGCCAATTCACATTATTTAGGCTATGTCAGCCGGGTGCAAAGCTTAGCTAACCAATTCGAAGCCGCTAC
TAGTTTTATTAACCCAGAAATCTTGAGCATCCCTAGTGATAAACTAGCTGAATTTAAGCAAGCTGAGCCAAGACTAAAGA
ATTATGCTCACTACTTGGAAATGATTACTAATAAGCGACCTCATACCCTGCCAGCTGAACAAGAAAAGTTAATCGCTGAT
GCTGGAGATGCTTTAGGCGTTTCCGAAAATACTTTTAACGTTTTAACTAACTCTGACATGGAATACGGCTACGTCCAAGA
TGACGATGGCAACATGGAACAACTATCTGACGGTTTGTACTCATTATTAATTCAATCGCAAAACCGTGACGTCAGAAAAG
GTGCCTTCGACACTCTCTATGCTACGTACAGCCAATTCCAGAATTCGCTGGCTTCAACCTTGTCCGGCGTAGTGAAAAAA
CACAACTATAACGCTAAAGCGCACAAATATGACTCTGCCCGTCAAGCAGCACTGGCTGAAAACGGCGTACCTGAAGCAGT
TTACGATACTTTAATTCAAGAAGTTGATTCTCACCTTGACCTGCTCCATCGTTACGTTGCCTTGCGCAAGAAAATTTTAG
GCTTAAAAGATTTGCAAATGTGGGACATGTACGTGCCGCTAACTGGGAAGCCTTCTCTTTCTTATAACTTTGAAGAAGCT
AAGGAAGTTGCTAAAAAGGCATTGAAGCCTTTGGGCGAAGACTACTTGAAGCATGTTGACTATATTTTTAATAATCGTGT
CATCGACCCAATAGAATCTAAAAACAAGGTAACTGGTGCCTACTCCGGTGGTGCTTATGATACCGATCCTTATGAGTTGC
TCAACTGGGAAGATAACATCGATTCACTTTATACTTTAGTCCACGAAACTGGTCACTCCGTTCACTCCTGGTACACTCGC
AATACTCAACCTTACGTTTACGGCGACTATCCAATCTTTGTGGCTGAAATTGCTTCAACTACTAATGAAAATATTTTGAC
CGAATATTTCTTAGACCACATTACCGATCCTAAGACGCGGGCCTTTATCTTAAATTACTACCTTGATTCATTTAAAGGCA
CTTTATTCCGTCAAACTCAATTTGCCGTGTTTGAACAATTCTTGCACGAAGCCGATGCTAATGGAGAACCACTGACCGCT
GACACTTTAGACGACGTTTACGGTCAAATTAATCAGCATTATTACGGCGATAGCGTTGAACCAGGCGGCGATATTGCCCT
TGAATGGTCACGAATTCCACACTTCTACTACAATTTCTATGTGTACCAATATGCGACTGGCTTTGCGGCTGCAACAGCAT
TAGCAAACAAGGTGGTTCATGGAACGCCTGCTGAGAGGGATGCCTACCTTGGCTTTCTTAAAGCTGGTTCAAGTGATTAC
CCAACTGAGATCATGAAGCGTGCTGGCGTAGACATGACTAAGCCTGACTACTTAGAAGATGCCTTCAAGACTTTTGAAAA
ACGGTTGAACGAATTTGAAAGCTTGATTGAGAAATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(113-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.347

99.331

0.51