Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW60_RS08290 Genome accession   NZ_CP118065
Coordinates   1669000..1670811 (+) Length   603 a.a.
NCBI ID   WP_049161077.1    Uniprot ID   -
Organism   Lactobacillus gasseri strain VSI26     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1664000..1675811
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW60_RS08265 (PUW60_08265) - 1664362..1665144 (-) 783 WP_003651264.1 threonine/serine exporter family protein -
  PUW60_RS08270 (PUW60_08270) - 1665167..1666714 (-) 1548 WP_003651262.1 ABC-F family ATP-binding cassette domain-containing protein -
  PUW60_RS08275 (PUW60_08275) - 1666723..1667115 (-) 393 WP_003649972.1 hypothetical protein -
  PUW60_RS08280 (PUW60_08280) - 1667338..1667607 (+) 270 WP_020807069.1 hypothetical protein -
  PUW60_RS08285 (PUW60_08285) - 1667712..1668836 (-) 1125 WP_003649969.1 LCP family protein -
  PUW60_RS08290 (PUW60_08290) pepF 1669000..1670811 (+) 1812 WP_049161077.1 oligoendopeptidase F Regulator
  PUW60_RS08295 (PUW60_08295) - 1670870..1671742 (-) 873 WP_003652430.1 Rpn family recombination-promoting nuclease/putative transposase -
  PUW60_RS08300 (PUW60_08300) - 1671886..1673397 (-) 1512 WP_035435517.1 lactate permease LctP family transporter -
  PUW60_RS08305 (PUW60_08305) - 1673517..1674308 (-) 792 WP_003649965.1 metal ABC transporter permease -
  PUW60_RS08310 (PUW60_08310) - 1674308..1674967 (-) 660 WP_003649964.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 69010.90 Da        Isoelectric Point: 4.6562

>NTDB_id=789749 PUW60_RS08290 WP_049161077.1 1669000..1670811(+) (pepF) [Lactobacillus gasseri strain VSI26]
MALPTRNEVSDDLKWDLSRVFKNDQEWEQEYKQVAQEIKNLSKFKGTLAKSGKDLYEGITAILAVNRRLEKVYVYATMSS
DVDTSNNHYLGFVAKAQSLANQMSAAIAFVDPEILSIPEETLAKFMQDEPRLENYRHRLEQITQKRPHTLPANEEKIIAD
AGDAMGTSANTFNVLTNSDMEYGYVQDEDGEMVQLSDGLYSLLIQSQDRNVRKNAFDVMYASYGQFENSLASTLSGEVKA
HNFNARVHKYNSAREAALSENSVPTAVYDTLIKEVNSHLDLLHRYVALRKKILGLKDLQMYDMYVPLTGKPVLSYNFEEA
KEEARKALAPLGEDYLKHVDYIFNNRVIDVVENQNKVTGAYSGGAYDTDPYELLNWEDNLDSLYTLVHETGHSVHSWYTR
NTQPYVYGDYPIFVAEIASTTNENILTEYFLDKITDPKTRAFVLNHYLDSFKGTLFRQTQFAEFEQFIHETDANGQPLTA
DVLDEFYGNLNQRYYGDSVEPGGEIAMEWSRIPHFYYNFYVYQYATGFAAATALANKVVHGTEQERDAYINFLKSGSSDY
PTEIMKRAGVDMTKADYLRDAFDTFEKRLNEFEKIVNELNAEK

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=789749 PUW60_RS08290 WP_049161077.1 1669000..1670811(+) (pepF) [Lactobacillus gasseri strain VSI26]
ATGGCTCTTCCAACAAGAAATGAAGTATCCGATGATCTTAAGTGGGACTTAAGTCGTGTTTTTAAGAACGATCAAGAATG
GGAACAAGAATATAAGCAGGTTGCACAAGAAATCAAGAACTTAAGTAAATTTAAGGGAACACTTGCCAAATCTGGTAAAG
ATCTTTACGAAGGAATTACAGCAATTTTAGCTGTAAATCGACGCTTAGAAAAAGTATATGTTTACGCTACAATGTCAAGC
GATGTAGACACAAGCAATAATCATTACCTCGGTTTTGTTGCTAAAGCACAAAGCTTAGCTAATCAAATGAGTGCTGCAAT
TGCTTTTGTCGATCCTGAAATATTATCTATTCCAGAAGAAACCCTTGCTAAGTTTATGCAAGATGAACCACGTTTAGAAA
ATTATCGACACCGCCTTGAACAGATTACGCAAAAACGTCCTCATACCTTGCCAGCAAATGAAGAAAAAATCATTGCTGAC
GCTGGAGATGCAATGGGTACATCTGCTAATACCTTCAACGTCTTAACTAATTCTGATATGGAATATGGCTACGTCCAAGA
TGAAGACGGTGAAATGGTTCAATTATCTGACGGGCTATACTCACTTTTAATTCAATCTCAAGACCGCAACGTCCGTAAAA
ACGCCTTTGACGTGATGTATGCAAGCTACGGACAGTTTGAAAACAGTCTTGCTTCAACTTTATCAGGTGAGGTAAAAGCT
CACAATTTTAATGCTCGCGTACATAAATACAATTCGGCTAGAGAAGCTGCTTTAAGTGAAAATAGTGTACCAACAGCTGT
TTACGATACATTGATCAAGGAAGTAAATAGCCACCTTGACTTACTTCACCGTTACGTTGCATTAAGAAAGAAAATTCTAG
GCTTAAAAGACCTTCAAATGTACGATATGTACGTCCCATTAACTGGGAAGCCAGTTCTTTCTTATAACTTTGAGGAAGCA
AAAGAAGAAGCAAGAAAGGCTCTAGCACCATTAGGAGAAGACTATTTAAAACACGTTGATTATATTTTTAATAACCGCGT
AATTGATGTGGTTGAAAATCAAAACAAGGTTACTGGTGCATATTCAGGTGGTGCTTACGATACTGATCCATATGAACTAC
TTAACTGGGAAGATAACCTTGATTCACTCTACACTCTAGTTCATGAAACTGGTCACTCTGTCCACTCTTGGTACACAAGA
AACACTCAACCTTATGTCTATGGTGATTATCCAATTTTCGTTGCTGAAATTGCTTCAACTACTAATGAGAATATTTTAAC
CGAGTATTTCTTAGATAAAATTACTGATCCAAAGACCCGCGCATTTGTCTTAAACCATTATCTGGATTCGTTTAAAGGCA
CACTCTTCCGTCAAACTCAATTTGCGGAATTTGAACAATTTATTCATGAAACTGATGCTAATGGTCAGCCGTTAACAGCT
GACGTACTCGATGAATTTTATGGCAACTTAAACCAAAGATACTATGGAGATAGCGTAGAACCTGGTGGAGAAATTGCTAT
GGAATGGTCACGTATTCCTCACTTCTACTACAACTTCTACGTTTACCAATACGCAACAGGTTTTGCAGCTGCAACTGCTC
TTGCTAATAAAGTTGTTCACGGCACTGAGCAAGAACGTGATGCTTATATTAATTTCTTAAAATCAGGCTCTTCAGATTAC
CCAACAGAAATTATGAAACGAGCTGGCGTTGATATGACTAAGGCTGATTACTTGAGGGATGCTTTTGATACTTTTGAAAA
ACGTCTTAATGAATTTGAAAAAATCGTTAATGAATTAAACGCTGAAAAGTAG

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(113-181)

Peptidase family M3 (Peptidase_M3)

(203-583)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

51.946

98.01

0.509