Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PUW68_RS10975 Genome accession   NZ_CP118060
Coordinates   2219816..2221180 (-) Length   454 a.a.
NCBI ID   WP_011276735.1    Uniprot ID   A0A2A1KAD2
Organism   Staphylococcus haemolyticus strain VSI35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2214816..2226180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW68_RS10960 (PUW68_10960) cysE 2215740..2216369 (-) 630 WP_176744743.1 serine O-acetyltransferase -
  PUW68_RS10965 (PUW68_10965) gltX 2216824..2218278 (-) 1455 WP_240566263.1 glutamate--tRNA ligase -
  PUW68_RS10970 (PUW68_10970) - 2218723..2219790 (-) 1068 WP_053025233.1 PIN/TRAM domain-containing protein -
  PUW68_RS10975 (PUW68_10975) radA 2219816..2221180 (-) 1365 WP_011276735.1 DNA repair protein RadA Machinery gene
  PUW68_RS10980 (PUW68_10980) - 2221402..2223876 (-) 2475 WP_070443016.1 ATP-dependent Clp protease ATP-binding subunit -
  PUW68_RS10985 (PUW68_10985) - 2223892..2224899 (-) 1008 WP_242305323.1 protein arginine kinase -
  PUW68_RS10990 (PUW68_10990) - 2224892..2225455 (-) 564 WP_242305325.1 UvrB/UvrC motif-containing protein -
  PUW68_RS10995 (PUW68_10995) - 2225478..2225939 (-) 462 WP_070443018.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49851.15 Da        Isoelectric Point: 7.0044

>NTDB_id=789731 PUW68_RS10975 WP_011276735.1 2219816..2221180(-) (radA) [Staphylococcus haemolyticus strain VSI35]
MAKKKVIFECMACGYQSPKWMGKCPNCGSWNQMEEIVEKASNPKHGVKTSTSSAKVQKLNDIKNEVTPRVQTESKEFNRV
LGGGIVSGSLVLIGGDPGIGKSTLLLQICASLSQKLNVLYITGEESLNQTKLRADRLDEDSSNLNVLAETDLEVIHQTVQ
QAQPDILVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGDE
HHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVHNPSEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPR
RMATGIDHNRLSLLMAVLEKKENYLLQQQDAYIKVAGGVRLTEPAVDLSVIVATASSFKDKAVDGLDCYIGEVGLTGEVR
RVSRIEQRVQEAAKLGFERVIIPQTNIGGWQFPEGIKVVGVSTVHDALKYAFKS

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=789731 PUW68_RS10975 WP_011276735.1 2219816..2221180(-) (radA) [Staphylococcus haemolyticus strain VSI35]
TTGGCCAAAAAGAAAGTGATTTTTGAGTGTATGGCTTGCGGCTATCAATCTCCAAAATGGATGGGAAAGTGTCCTAATTG
TGGGTCATGGAATCAAATGGAGGAAATTGTTGAAAAAGCTTCAAATCCTAAACACGGCGTAAAGACATCAACGTCTAGCG
CAAAAGTCCAGAAATTAAATGATATCAAGAATGAAGTGACACCGAGAGTTCAGACAGAATCAAAAGAGTTCAACCGCGTT
TTAGGTGGTGGCATTGTAAGTGGCTCTTTAGTGTTAATAGGTGGAGATCCTGGTATCGGTAAATCAACGTTATTGCTGCA
AATTTGTGCATCATTGTCACAAAAATTAAATGTACTGTATATTACAGGTGAAGAATCACTAAATCAAACTAAATTACGTG
CCGATCGACTAGATGAAGATTCGAGTAATTTAAATGTGTTAGCAGAAACAGATTTAGAGGTAATACATCAAACAGTTCAA
CAAGCACAACCTGATATTTTAGTTGTGGATTCCATTCAAACAATATATCATCCTGAAATTAGTTCGGCACCTGGTTCTGT
GTCTCAAGTTAGAGAAAGTACACAGAGTTTAATGAATATTGCTAAACAAATGAATATTGCAACATTTATCGTCGGACATG
TTACAAAAGAAGGTCAAATTGCAGGACCGCGTCTATTAGAACATATGGTTGATACTGTACTGTACTTTGAGGGAGATGAG
CATCATGCTTATCGTATCTTGAGAGCGGTTAAGAACCGTTTTGGTTCTACTAATGAAATGGGCATTTTTGAGATGAAACA
AAGCGGGTTAAAAGGCGTTCATAATCCTTCTGAAATGTTCTTAGAGGAACGTTCTACCAATGTGCCGGGATCAACGATTG
TAGCAACTATGGAAGGTACACGACCTCTACTAATAGAAGTTCAGGCCTTAGTGACGCCTACAACATTTAATAACCCTAGA
CGTATGGCAACAGGTATAGATCATAATCGTTTGAGTTTATTAATGGCTGTATTAGAGAAAAAAGAAAACTATTTATTACA
ACAACAAGATGCATATATTAAAGTAGCTGGTGGCGTTCGATTGACAGAGCCGGCAGTTGATTTAAGTGTTATAGTAGCAA
CAGCTTCAAGTTTTAAAGATAAAGCAGTAGATGGGCTTGATTGTTATATTGGAGAAGTAGGTTTGACCGGTGAAGTGAGA
CGTGTTTCTCGAATTGAACAACGTGTTCAAGAAGCAGCTAAATTAGGCTTTGAACGTGTTATTATTCCACAGACAAATAT
CGGTGGATGGCAATTTCCTGAGGGCATTAAAGTAGTAGGAGTGTCTACAGTACATGATGCACTAAAATATGCATTCAAAT
CGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(67-218)

Subunit ChlI of Mg-chelatase (ChlI)

(359-426)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A1KAD2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

65.427

100

0.659
  radA Streptococcus pneumoniae Rx1

60.177

99.559

0.599
  radA Streptococcus pneumoniae D39

60.177

99.559

0.599
  radA Streptococcus pneumoniae R6

60.177

99.559

0.599
  radA Streptococcus pneumoniae TIGR4

60.177

99.559

0.599
  radA Streptococcus mitis NCTC 12261

60.177

99.559

0.599
  radA Streptococcus mitis SK321

60.177

99.559

0.599