Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PUW71_RS00950 Genome accession   NZ_CP118053
Coordinates   169697..171115 (+) Length   472 a.a.
NCBI ID   WP_101749836.1    Uniprot ID   -
Organism   Streptococcus anginosus strain VSI40     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 164697..176115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW71_RS00930 (PUW71_00930) dinB 165305..166372 (+) 1068 WP_150888993.1 DNA polymerase IV -
  PUW71_RS00935 (PUW71_00935) - 166637..167837 (-) 1201 Protein_147 IS110 family transposase -
  PUW71_RS00940 (PUW71_00940) - 168169..169200 (+) 1032 WP_150888890.1 S66 peptidase family protein -
  PUW71_RS00945 (PUW71_00945) - 169294..169737 (+) 444 WP_003027048.1 dUTP diphosphatase -
  PUW71_RS00950 (PUW71_00950) radA 169697..171115 (+) 1419 WP_101749836.1 DNA repair protein RadA Machinery gene
  PUW71_RS00955 (PUW71_00955) gltX 171323..172780 (+) 1458 WP_150888886.1 glutamate--tRNA ligase -
  PUW71_RS00960 (PUW71_00960) dapD 172895..173582 (+) 688 Protein_152 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-acetyltransferase -
  PUW71_RS00965 (PUW71_00965) - 173595..174726 (+) 1132 Protein_153 N-acetyldiaminopimelate deacetylase -
  PUW71_RS00970 (PUW71_00970) - 174771..175298 (+) 528 WP_150888884.1 5-formyltetrahydrofolate cyclo-ligase -
  PUW71_RS00975 (PUW71_00975) - 175291..175962 (+) 672 WP_150888882.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 51494.27 Da        Isoelectric Point: 5.8400

>NTDB_id=789574 PUW71_RS00950 WP_101749836.1 169697..171115(+) (radA) [Streptococcus anginosus strain VSI40]
MVCGQVVLEARGIKKEESIIAKKKTTFVCQNCEYHSPKYLGRCPNCGAWSSFVEEIEVAEVKNARVSLTGEKSRPMKLAE
VASINVNRTKTDMDEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSHKGTVLYVSGEESAEQIKLRAERLGDID
SEFYLYAETNMQQIRAEIEKIKPDFLIIDSIQTIISPEISSVQGSVSQVREVTAELMQLAKANNIATFIVGHMTKEGTLA
GPRTLEHMVDTVLYFEGERQHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTR
PILAEVQALVTPTMFGNAKRTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDL
PTNPQECFIGEIGLTGEIRRVNRIEQRINEAAKLGFTKVYAPKNSLNGLNIPETIEIIGVTTIGEVLKKVFG

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=789574 PUW71_RS00950 WP_101749836.1 169697..171115(+) (radA) [Streptococcus anginosus strain VSI40]
ATGGTGTGCGGACAGGTGGTTTTGGAAGCACGGGGCATTAAGAAAGAGGAATCTATCATAGCCAAGAAAAAAACGACATT
TGTTTGTCAAAATTGTGAATACCATTCGCCCAAGTATCTAGGGCGCTGTCCTAACTGTGGGGCTTGGTCTTCTTTTGTAG
AAGAGATCGAAGTTGCAGAGGTTAAGAATGCGCGTGTTTCTCTGACAGGTGAAAAAAGTCGACCGATGAAGTTAGCGGAG
GTGGCTTCTATCAATGTCAACCGTACCAAGACAGATATGGATGAGTTTAACCGCGTCTTGGGTGGTGGCGTGGTGCCAGG
AAGTTTAGTTCTAATCGGAGGAGATCCCGGTATTGGGAAATCTACCCTTTTATTGCAGGTTTCGACCCAACTATCTCACA
AGGGAACGGTATTGTATGTCAGTGGGGAAGAGTCTGCTGAGCAGATTAAGTTGCGTGCGGAGCGATTAGGCGATATTGAC
AGTGAGTTTTATCTTTATGCCGAGACCAATATGCAGCAGATTCGTGCTGAGATTGAAAAAATTAAACCAGATTTCTTGAT
TATAGACTCTATTCAGACGATTATTTCGCCAGAAATTTCCAGCGTGCAAGGGTCGGTTTCACAAGTCAGAGAAGTGACAG
CGGAGCTGATGCAGTTGGCAAAAGCCAACAATATTGCCACCTTTATCGTGGGCCATATGACCAAGGAAGGAACGCTGGCT
GGACCTAGAACCTTGGAACACATGGTGGATACGGTACTTTATTTTGAAGGTGAGCGACAGCATACTTTTCGTATTTTGCG
TGCGGTCAAAAATCGCTTCGGCTCTACGAATGAAATCGGCATTTTTGAGATGCAGTCAGGTGGGCTGGTTGAAGTGCTCA
ATCCTAGTCAAGTTTTCTTAGAGGAGCGTTTAGATGGGGCAACAGGCTCTTCTATCGTGGTGACTATGGAGGGGACGCGT
CCAATCCTTGCGGAAGTACAAGCTTTGGTGACACCGACCATGTTTGGCAATGCCAAGCGGACAACGACAGGATTGGATTT
CAATCGTGCTAGTCTGATTATGGCTGTTTTGGAAAAGCGAGCAGGGTTATTATTGCAAAATCAAGATGCCTACCTCAAGT
CAGCAGGCGGTGTCAAATTAGATGAGCCAGCCATTGACTTAGCTGTTGCTGTTGCAATTGCTTCTAGTTATAAAGATTTG
CCAACGAATCCACAAGAATGTTTTATTGGTGAAATCGGTCTGACGGGCGAAATCCGCCGTGTCAATCGCATTGAGCAGCG
TATCAATGAAGCTGCTAAGCTGGGCTTCACCAAAGTTTACGCTCCTAAAAACTCTCTGAACGGGTTGAACATTCCCGAAA
CTATTGAAATTATTGGTGTAACAACGATTGGTGAAGTGTTGAAGAAGGTGTTTGGGTAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(380-444)

AAA domain (AAA_25)

(84-237)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(27-54)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

91.15

95.763

0.873
  radA Streptococcus pneumoniae D39

91.15

95.763

0.873
  radA Streptococcus pneumoniae R6

91.15

95.763

0.873
  radA Streptococcus pneumoniae TIGR4

91.15

95.763

0.873
  radA Streptococcus mitis SK321

91.15

95.763

0.873
  radA Streptococcus mitis NCTC 12261

90.929

95.763

0.871
  radA Bacillus subtilis subsp. subtilis str. 168

61.926

96.822

0.6