Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PUW76_RS09885 Genome accession   NZ_CP118048
Coordinates   2098720..2100531 (-) Length   603 a.a.
NCBI ID   WP_002382553.1    Uniprot ID   A0A7H0FMB4
Organism   Enterococcus faecalis strain VSI48     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 2093720..2105531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW76_RS09860 (PUW76_09860) - 2094745..2095638 (-) 894 WP_002362547.1 RluA family pseudouridine synthase -
  PUW76_RS09865 (PUW76_09865) - 2095646..2096443 (-) 798 WP_002356486.1 NAD kinase -
  PUW76_RS09870 (PUW76_09870) - 2096456..2097130 (-) 675 WP_002356484.1 GTP pyrophosphokinase family protein -
  PUW76_RS09875 (PUW76_09875) - 2097327..2097905 (+) 579 WP_002356483.1 CYTH domain-containing protein -
  PUW76_RS09880 (PUW76_09880) - 2097969..2098628 (+) 660 WP_002356482.1 ClpXP adapter SpxH family protein -
  PUW76_RS09885 (PUW76_09885) pepF 2098720..2100531 (-) 1812 WP_002382553.1 oligoendopeptidase F Regulator
  PUW76_RS09890 (PUW76_09890) - 2100579..2101757 (-) 1179 WP_033601133.1 competence protein CoiA family protein -
  PUW76_RS09895 (PUW76_09895) - 2101847..2102509 (-) 663 WP_002356478.1 adaptor protein MecA -
  PUW76_RS09900 (PUW76_09900) spxA 2102852..2103250 (-) 399 WP_002356477.1 transcriptional regulator SpxA -
  PUW76_RS09905 (PUW76_09905) trpS 2103610..2104620 (+) 1011 WP_002359559.1 tryptophan--tRNA ligase -

Sequence

Protein


Download         Length: 603 a.a.        Molecular weight: 68907.19 Da        Isoelectric Point: 4.6882

>NTDB_id=789531 PUW76_RS09885 WP_002382553.1 2098720..2100531(-) (pepF) [Enterococcus faecalis strain VSI48]
MSEIKQLPTRDEVPTPLTWDLTKIFKDDAAFDVAYNQLTEELNQAESFKGTLGNGAEAFLAALEYVLDVYRKVETLYVYS
HLKNDQDTTNTAYQALYARASSLYAQVSEAVSWFDPEVLTLSDEQIWGYFEEQPKLAVYRHYIQNILDERPHVLSMEQEA
LLAGASEIFGASSNTFSILNNADLEFPTVQNAEGETIQLSHGVYGQLMESVDPSVREAAFKGLYKVYKQFRNTLASTLGA
HVKTHNYKAKIRNYDSARAASLASNHIPESVHETLVAVVNKHLPLLHRYVKLRKKLLNVEELHMYDLYAPLLGEAPIRYS
YEEAKEKAIEALKPLGEDYLSIVKEAFSSRWIDVIENQGKRSGAYSSGAYDTAPYILMNWHDSLDQLFTLVHEMGHSVHS
YYTRNNQPYVYGDYSIFLAEIASTTNENILTEYLLQTETDPKVRAYVLNHYLDGFKGTIFRQTQFAEFEHFIHTEDAKGT
PLTSEYLSEYYGELNAKYYGPEVVRDEEISYEWARIPHFYYNYYVYQYATGFSAASALSKHILAEEEGALENYLNYLKAG
SSDFPIEVMKKAGVDMTQAAYIEDAMKVFEERLTELEALVEKL

Nucleotide


Download         Length: 1812 bp        

>NTDB_id=789531 PUW76_RS09885 WP_002382553.1 2098720..2100531(-) (pepF) [Enterococcus faecalis strain VSI48]
ATGAGCGAAATTAAACAATTACCAACACGTGATGAAGTTCCAACTCCATTGACTTGGGATTTAACCAAAATTTTTAAAGA
TGATGCTGCTTTTGATGTTGCGTATAATCAGTTGACAGAGGAGCTAAATCAAGCAGAGTCATTTAAAGGTACCTTAGGGA
ATGGCGCAGAAGCTTTTCTAGCTGCGTTAGAATACGTTTTAGATGTCTATCGAAAAGTGGAAACATTATATGTCTATTCT
CATTTAAAAAATGATCAAGACACTACAAATACAGCTTACCAAGCTTTATATGCCAGAGCTAGTTCTTTATATGCACAAGT
TAGTGAAGCTGTTTCTTGGTTTGACCCAGAGGTTTTAACATTAAGTGATGAACAAATCTGGGGATATTTTGAAGAGCAGC
CGAAATTAGCTGTTTATCGTCACTATATTCAAAATATTTTAGATGAACGTCCCCATGTTTTATCGATGGAACAAGAAGCT
TTACTGGCGGGGGCTAGTGAAATTTTTGGTGCCTCAAGTAATACATTTTCAATTTTAAATAATGCAGACTTAGAATTTCC
AACTGTGCAAAATGCTGAAGGCGAAACGATTCAACTTTCTCATGGCGTTTATGGTCAATTAATGGAAAGTGTCGATCCAT
CTGTTCGTGAAGCAGCATTTAAAGGGTTGTACAAAGTGTACAAACAATTTAGAAATACATTAGCCTCAACTTTAGGTGCA
CATGTTAAAACGCATAATTATAAAGCAAAAATTAGAAATTATGATTCTGCTCGGGCAGCCTCTTTAGCAAGTAATCATAT
CCCTGAAAGTGTTCACGAAACATTAGTAGCTGTAGTAAATAAACATTTACCTTTGCTACATCGTTATGTAAAATTAAGAA
AAAAATTATTAAACGTAGAAGAATTACACATGTACGACTTGTATGCGCCTTTGCTAGGTGAAGCACCAATTCGTTACAGC
TATGAAGAAGCAAAAGAAAAAGCAATTGAGGCTTTAAAACCACTAGGTGAAGACTATTTATCTATTGTTAAAGAAGCTTT
TTCAAGTCGTTGGATTGATGTGATTGAAAATCAAGGAAAACGAAGCGGCGCATATTCTTCAGGAGCTTATGATACAGCCC
CATACATTTTAATGAATTGGCATGATAGTTTGGATCAACTATTTACATTAGTCCATGAGATGGGCCATAGTGTTCATAGT
TACTATACAAGAAATAATCAGCCGTATGTTTATGGCGACTATTCAATTTTCTTAGCTGAGATTGCTTCAACCACAAATGA
AAATATTTTAACAGAATATTTATTACAAACAGAAACAGATCCTAAAGTACGTGCGTATGTCTTAAATCACTATTTAGACG
GCTTTAAGGGAACCATTTTCCGTCAAACGCAATTTGCGGAATTTGAACATTTTATTCACACGGAAGATGCTAAAGGCACG
CCATTAACGAGTGAATATTTGAGTGAGTATTATGGCGAGCTCAATGCTAAATATTATGGACCAGAAGTAGTTAGAGACGA
AGAAATCAGTTACGAATGGGCGAGAATTCCCCATTTTTATTACAATTACTATGTTTATCAATATGCAACTGGCTTCTCAG
CCGCTTCGGCATTGTCTAAACATATTTTAGCTGAAGAAGAGGGAGCTTTAGAGAACTATCTTAACTACTTGAAAGCAGGA
AGTAGTGACTTCCCAATTGAAGTGATGAAAAAAGCGGGCGTGGATATGACGCAAGCCGCGTATATCGAAGATGCAATGAA
AGTTTTTGAAGAACGTTTAACGGAATTAGAAGCTTTGGTTGAAAAATTATAG

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(117-186)

Peptidase family M3 (Peptidase_M3)

(207-587)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7H0FMB4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

54.469

98.342

0.536