Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PUW46_RS07365 Genome accession   NZ_CP118044
Coordinates   1542515..1543585 (-) Length   356 a.a.
NCBI ID   WP_000776401.1    Uniprot ID   -
Organism   Staphylococcus aureus strain VSI56     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1537515..1548585
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW46_RS07325 (PUW46_07325) gcvPA 1537692..1539038 (-) 1347 WP_275000702.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  PUW46_RS07330 (PUW46_07330) gcvT 1539058..1540149 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  PUW46_RS07335 (PUW46_07335) - 1540308..1540832 (-) 525 WP_001015121.1 shikimate kinase -
  PUW46_RS07340 (PUW46_07340) - 1540822..1540968 (-) 147 WP_001789879.1 hypothetical protein -
  PUW46_RS07345 (PUW46_07345) comGF 1541065..1541562 (-) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  PUW46_RS07350 (PUW46_07350) comGE 1541480..1541779 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  PUW46_RS07355 (PUW46_07355) comGD 1541766..1542212 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  PUW46_RS07360 (PUW46_07360) comGC 1542190..1542501 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  PUW46_RS07365 (PUW46_07365) comGB 1542515..1543585 (-) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  PUW46_RS07370 (PUW46_07370) comGA 1543557..1544531 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  PUW46_RS07375 (PUW46_07375) - 1544583..1545206 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  PUW46_RS07380 (PUW46_07380) - 1545203..1545532 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  PUW46_RS07385 (PUW46_07385) - 1545532..1546518 (-) 987 WP_000161314.1 ROK family glucokinase -
  PUW46_RS07390 (PUW46_07390) - 1546515..1546718 (-) 204 WP_000087561.1 YqgQ family protein -
  PUW46_RS07395 (PUW46_07395) - 1546699..1548162 (-) 1464 WP_001017871.1 rhomboid family intramembrane serine protease -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41508.88 Da        Isoelectric Point: 9.9593

>NTDB_id=789417 PUW46_RS07365 WP_000776401.1 1542515..1543585(-) (comGB) [Staphylococcus aureus strain VSI56]
MKLQWINTFKLHFEKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=789417 PUW46_RS07365 WP_000776401.1 1542515..1543585(-) (comGB) [Staphylococcus aureus strain VSI56]
GTGAAACTACAATGGATAAATACATTTAAACTACATTTTGAGAAGCGACAATTAAGTAAGGCCCAACAAATCGATTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACTGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGACACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994