Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   PT671_RS17800 Genome accession   NZ_CP118021
Coordinates   3557506..3558273 (+) Length   255 a.a.
NCBI ID   WP_003226211.1    Uniprot ID   E0U4P1
Organism   Bacillus spizizenii strain B-354     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 3552506..3563273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS17765 - 3552724..3552858 (+) 135 WP_014114453.1 hypothetical protein -
  PT671_RS17770 pgpH 3552803..3554938 (+) 2136 WP_079996420.1 cyclic-di-AMP phosphodiesterase PgpH -
  PT671_RS17775 ybeY 3554939..3555412 (+) 474 WP_003226199.1 rRNA maturation RNase YbeY -
  PT671_RS17780 - 3555393..3555764 (+) 372 WP_003226203.1 diacylglycerol kinase family protein -
  PT671_RS17785 - 3555885..3556295 (+) 411 WP_003226204.1 cytidine deaminase -
  PT671_RS17790 era 3556276..3557181 (+) 906 WP_003226207.1 GTPase Era -
  PT671_RS17795 - 3557326..3557469 (+) 144 WP_003230053.1 YqzL family protein -
  PT671_RS17800 recO 3557506..3558273 (+) 768 WP_003226211.1 DNA repair protein RecO Machinery gene
  PT671_RS17805 glyQ 3558570..3559457 (+) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  PT671_RS17810 glyS 3559450..3561489 (+) 2040 WP_003226215.1 glycine--tRNA ligase subunit beta -
  PT671_RS17815 ccpN 3561623..3562261 (+) 639 WP_003226217.1 transcriptional regulator CcpN -
  PT671_RS17820 - 3562287..3563099 (+) 813 WP_003226219.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29305.97 Da        Isoelectric Point: 8.3072

>NTDB_id=789183 PT671_RS17800 WP_003226211.1 3557506..3558273(+) (recO) [Bacillus spizizenii strain B-354]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDKGTEEKKPNPYLFEFILESFKQLDEGTDPDVITFIAQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRVPIKPQTARLLRLFYYFDLSRLGNVSLKQETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=789183 PT671_RS17800 WP_003226211.1 3557506..3558273(+) (recO) [Bacillus spizizenii strain B-354]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTTACATTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCAAGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCATTTTTAATGCAAAAAACATCAGGCCTTGGAACGCTTCAGCAGGGTGAAATGATCCTCAGTATGAGAGGC
ATCAGGGAAGATTTGTTTTTAACAGCATATGCTGCGTATATTGCTGAGCTAGTCGACAAAGGCACAGAAGAAAAGAAGCC
AAACCCTTATTTGTTTGAATTCATTTTAGAATCATTTAAACAGCTGGATGAAGGAACAGATCCGGATGTCATCACCTTCA
TTGCTCAAATGAAGATGCTCGGTGTCATGGGGCTGTATCCTGAGCTGAATCACTGTGTGCATTGTAAAAGCCAGGACGGA
ACCTTTCATTTTTCTGTTCGTGATAACGGTTTTATTTGCCATCGCTGCTTTGAAAAAGACCCTTACAGGGTTCCAATTAA
ACCGCAAACGGCAAGGCTTTTGAGACTGTTTTATTACTTCGATCTATCGAGGCTGGGCAATGTGTCCTTAAAGCAGGAAA
CAAAAGCTGAATTAAAGCAAGTAATTGATTTGTATTATGAAGAATATTCAGGGTTATATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTAATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U4P1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

97.255

100

0.973