Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PT671_RS17545 Genome accession   NZ_CP118021
Coordinates   3514370..3514780 (+) Length   136 a.a.
NCBI ID   WP_003226105.1    Uniprot ID   E0U4U4
Organism   Bacillus spizizenii strain B-354     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3509370..3519780
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT671_RS17510 - 3509715..3509851 (+) 137 Protein_3400 hypothetical protein -
  PT671_RS17515 - 3509809..3509973 (+) 165 Protein_3401 recombinase family protein -
  PT671_RS17520 - 3510405..3511295 (-) 891 WP_003226089.1 LysR family transcriptional regulator -
  PT671_RS17525 fumC 3511450..3512838 (+) 1389 WP_003226090.1 class II fumarate hydratase -
  PT671_RS17530 - 3513004..3513140 (+) 137 Protein_3404 hypothetical protein -
  PT671_RS17535 - 3513098..3513289 (+) 192 Protein_3405 recombinase -
  PT671_RS17540 sigK 3513446..3514174 (-) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  PT671_RS17545 nucA/comI 3514370..3514780 (+) 411 WP_003226105.1 sporulation-specific Dnase NucB Machinery gene
  PT671_RS17550 - 3514813..3515538 (-) 726 WP_032727305.1 YqeB family protein -
  PT671_RS17555 gnd 3515840..3516733 (+) 894 WP_003226110.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  PT671_RS17560 - 3516752..3517378 (-) 627 WP_003226112.1 TVP38/TMEM64 family protein -
  PT671_RS17565 - 3517573..3518325 (+) 753 WP_003226114.1 peptidoglycan recognition protein family protein -
  PT671_RS17570 - 3518576..3519307 (+) 732 WP_003226118.1 SGNH/GDSL hydrolase family protein -
  PT671_RS17575 - 3519610..3519750 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14953.00 Da        Isoelectric Point: 5.1750

>NTDB_id=789179 PT671_RS17545 WP_003226105.1 3514370..3514780(+) (nucA/comI) [Bacillus spizizenii strain B-354]
MKKWMAGLFLAAAVLLCLMVPQQIQGASLYDKVLYFPLSRYPETGDHIKDAIAEGHSDICTIDRDGADKRRQESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSGYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=789179 PT671_RS17545 WP_003226105.1 3514370..3514780(+) (nucA/comI) [Bacillus spizizenii strain B-354]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTTGTATGACAAAGTGTTGTATTTTCCGCTGTCTCGTTATCCGGAAACGGGGGATCATATTAAAGATGCGATTGCAG
AGGGACATTCTGATATTTGCACTATCGACAGGGATGGAGCGGACAAAAGGCGGCAGGAATCATTAAAAGGAATTCCGACG
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTTTGTGAGGAAGGCGGCGCAGGAGCTGATGTCCGATATGTCAC
GCCTTCTGATAATCGCGGGGCCGGCTCGTGGGTAGGGAATCAAATGAGCGGTTATCCTGACGGCACCAGAGTGCTCTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E0U4U4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.478

84.559

0.537