Detailed information NTG0789134

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: PT671_RS04130
• Genome accession: NZ_CP118021
• Coordinates: 877554..881258 (-)
• Length: 1234 a.a.
• NCBI ID: WP_003224421.1
• Uniprot ID: -
• Organism: Bacillus spizizenii strain B-354
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 872554..886258

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PT671_RS04115	hlpB	872602..872904 (-)	303	WP_003224428.1	HNH nuclease-like protein HlpB	-
PT671_RS04120	sbcC	872918..876310 (-)	3393	WP_003224426.1	exonuclease subunit SbcC	-
PT671_RS04125	sbcD	876307..877482 (-)	1176	WP_003224424.1	exonuclease subunit SbcD	-
PT671_RS04130	addA	877554..881258 (-)	3705	WP_003224421.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
PT671_RS04135	addB	881245..884745 (-)	3501	WP_003224417.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
PT671_RS04140	-	884929..885366 (+)	438	WP_003224414.1	ferritin family protein	-
PT671_RS04145	-	885483..885812 (+)	330	WP_032727222.1	four-helix bundle copper-binding protein	-

Sequence

Protein

• Length: 1234 a.a.
• Molecular weight: 141455.81 Da
• Isoelectric Point: 5.0516

>NTDB_id=789134 PT671_RS04130 WP_003224421.1 877554..881258(-) (addA) [Bacillus spizizenii strain B-354]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDQLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIEMVLNGAKEKLQRA
LELTKVPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRVKAVKGDEFDPTLLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVRLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENGAATHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGTFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKTAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide

• Length: 3705 bp

>NTDB_id=789134 PT671_RS04130 WP_003224421.1 877554..881258(-) (addA) [Bacillus spizizenii strain B-354]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTAGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCAGCTTCTTGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAACGCCCCGGCTCGCTGCATATCAGGCGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATATGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGATCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGTGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGAGATGGTGCTTAACGGAGCGAAGGAAAAGCTTCAGCGCGCG
CTTGAACTGACGAAAGTGCCGGGCGGTCCCGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGATGATTTTACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGTGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTACACTCCTTGAGGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACGAGAAGTCCTGAACAGCATTTAAAAAGCCTAGCCGACATGAAGCCTGTTATTGAAACGCTCGTACG
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGAAACAGGTGAACGCGAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
TGAGGAAACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCAATTTACCGATTCAGGCTTGCGGAGCCGCTTCTAT
TCCTCTCCAAATACAAACGGTTTACAGAAAGTGGAGAAGGCACGGGACGGAAAATTGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTCTATTTAAACAACTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTTGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCACCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTACGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGGCATCTTCAAAAATGGCGTGCATTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGAGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGATTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTCGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTTAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACAAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAAAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAGACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATTGGACCGGCTCTTGCGAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGGTGCGGCAACGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTACTGGATGATGATCTGGAAGAAAGGACGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCACGTTTGCATTTGATGAAAAAGCCCGCGAGCAGTTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGCCCCGCTTTCATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACACATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAACCGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAAATCTATCCTGATGC
ACAGGAGGCAGATGAACCGCTTTTAGTGCAGGGGATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAGTTCCAACACGGCTTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTGTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (11-461)

• UvrD-like helicase C-terminal domain (UvrD_C) (472-882)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1055-1225)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	96.515	100	0.965