Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PUO93_RS03890 Genome accession   NZ_CP117993
Coordinates   650965..651945 (+) Length   326 a.a.
NCBI ID   WP_015571836.1    Uniprot ID   -
Organism   Enterobacter hormaechei isolate 1023017620-1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645965..656945
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO93_RS03850 - 646049..646375 (+) 327 WP_003862421.1 YggL family protein -
  PUO93_RS03855 - 646432..647148 (+) 717 WP_015571842.1 DUF2884 domain-containing protein -
  PUO93_RS03860 - 647232..647570 (-) 339 WP_015571841.1 endonuclease domain-containing protein -
  PUO93_RS03865 hemW 647627..648775 (-) 1149 WP_045141926.1 radical SAM family heme chaperone HemW -
  PUO93_RS03870 - 648768..649361 (-) 594 WP_015571839.1 XTP/dITP diphosphatase -
  PUO93_RS03875 yggU 649365..649661 (-) 297 WP_003860023.1 DUF167 family protein YggU -
  PUO93_RS03880 - 649658..650224 (-) 567 WP_015571838.1 YggT family protein -
  PUO93_RS03885 - 650246..650947 (-) 702 WP_015571837.1 YggS family pyridoxal phosphate-dependent enzyme -
  PUO93_RS03890 pilT 650965..651945 (+) 981 WP_015571836.1 type IV pilus twitching motility protein PilT Machinery gene
  PUO93_RS03895 ruvX 651955..652371 (-) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  PUO93_RS03900 - 652371..652931 (-) 561 WP_006811924.1 YqgE/AlgH family protein -
  PUO93_RS03905 gshB 653006..653953 (-) 948 WP_015571835.1 glutathione synthase -
  PUO93_RS03910 rsmE 653985..654716 (-) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PUO93_RS03915 endA 654768..655475 (-) 708 WP_003860034.1 deoxyribonuclease I -
  PUO93_RS03920 - 655570..656067 (-) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35690.68 Da        Isoelectric Point: 6.4131

>NTDB_id=788992 PUO93_RS03890 WP_015571836.1 650965..651945(+) (pilT) [Enterobacter hormaechei isolate 1023017620-1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMFQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=788992 PUO93_RS03890 WP_015571836.1 650965..651945(+) (pilT) [Enterobacter hormaechei isolate 1023017620-1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGCTAGG
TGTACCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCACCAGACGGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTTCCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
CAGCGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCGGAAACCGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACC
TTTCCGGCTCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

37.888

98.773

0.374