Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   PUO93_RS02645 Genome accession   NZ_CP117993
Coordinates   400229..401662 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei isolate 1023017620-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395229..406662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUO93_RS02615 elbB 396761..397414 (+) 654 WP_015571987.1 isoprenoid biosynthesis glyoxalase ElbB -
  PUO93_RS02620 mtgA 397411..398136 (+) 726 WP_015571986.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  PUO93_RS02625 npr 398176..398448 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  PUO93_RS02630 rapZ 398445..399299 (-) 855 WP_003861870.1 RNase adapter RapZ -
  PUO93_RS02635 ptsN 399345..399836 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  PUO93_RS02640 hpf 399919..400206 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  PUO93_RS02645 rpoN/rpoN1 400229..401662 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  PUO93_RS02650 lptB 401710..402435 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  PUO93_RS02655 lptA 402442..402996 (-) 555 WP_015571984.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  PUO93_RS02660 lptC 402965..403540 (-) 576 WP_015571983.1 LPS export ABC transporter periplasmic protein LptC -
  PUO93_RS02665 kdsC 403537..404103 (-) 567 WP_047051238.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  PUO93_RS02670 kdsD 404118..405104 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  PUO93_RS02675 - 405118..406095 (-) 978 WP_047051237.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=788991 PUO93_RS02645 WP_003861864.1 400229..401662(-) (rpoN/rpoN1) [Enterobacter hormaechei isolate 1023017620-1]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=788991 PUO93_RS02645 WP_003861864.1 400229..401662(-) (rpoN/rpoN1) [Enterobacter hormaechei isolate 1023017620-1]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTACAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAACTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAAACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCGCTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACACGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACCACTCAGTCGCTTCAGGACTACCTGATGTGGCAGGTAGAGCTGACCCCTTTCT
CGGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTGGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGCGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCCAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAATCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCCCTCGATCCGCGTCCGGGGCAGTCGATCCAGACCAG
CGAACCGGAATATGTCATTCCTGATGTGCTGGTGAGAAAACACAATGGCCGGTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCACGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATCAAGAGTCTGGAGAGTCGTAATGATACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCGTTCTTTGAACAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATCGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCAACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48