Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PUB83_RS10590 Genome accession   NZ_CP117918
Coordinates   2354302..2355441 (+) Length   379 a.a.
NCBI ID   WP_011706550.1    Uniprot ID   A0AAI9Q2R8
Organism   Aeromonas hydrophila subsp. hydrophila strain PSKL.DP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2349302..2360441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUB83_RS10560 (PUB83_08025) - 2349609..2350673 (+) 1065 WP_029303894.1 3-deoxy-7-phosphoheptulonate synthase -
  PUB83_RS10565 (PUB83_08030) aac(6') 2350680..2351138 (+) 459 WP_011706546.1 aminoglycoside 6'-N-acetyltransferase -
  PUB83_RS10570 (PUB83_08035) - 2351160..2351945 (-) 786 WP_274278570.1 ATP-binding cassette domain-containing protein -
  PUB83_RS10575 (PUB83_08040) btuC 2351932..2352939 (-) 1008 WP_103828718.1 vitamin B12 ABC transporter permease BtuC -
  PUB83_RS10580 (PUB83_08045) - 2353040..2353771 (-) 732 WP_045527346.1 ABC transporter substrate-binding protein -
  PUB83_RS10585 (PUB83_08050) - 2353885..2354241 (+) 357 WP_005302630.1 DMT family protein -
  PUB83_RS10590 (PUB83_08055) pilU 2354302..2355441 (+) 1140 WP_011706550.1 type IVa pilus ATPase TapW Machinery gene
  PUB83_RS10595 (PUB83_08060) glgC 2355724..2356995 (+) 1272 WP_011706551.1 glucose-1-phosphate adenylyltransferase -
  PUB83_RS10600 (PUB83_08065) - 2357131..2357610 (+) 480 WP_164927684.1 WbuC family cupin fold metalloprotein -
  PUB83_RS10605 (PUB83_08070) - 2357725..2357967 (-) 243 WP_011706553.1 FeoC-like transcriptional regulator -
  PUB83_RS10610 (PUB83_08075) feoB 2357964..2360237 (-) 2274 WP_043162936.1 Fe(2+) transporter permease subunit FeoB -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42264.32 Da        Isoelectric Point: 6.4290

>NTDB_id=788321 PUB83_RS10590 WP_011706550.1 2354302..2355441(+) (pilU) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETPLEPGEVARIADAIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTSDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIDEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKDDLF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=788321 PUB83_RS10590 WP_011706550.1 2354302..2355441(+) (pilU) [Aeromonas hydrophila subsp. hydrophila strain PSKL.DP]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGATGGCTCCGACCTCTATCTGTCGACCGGCGCCCCCCCTTG
CGCCAAGTTCAACGGCGGCCTGCGTCCGCTCAGTGAAACGCCGCTCGAGCCCGGCGAGGTGGCCAGAATAGCCGACGCCA
TCATGGACGGCGAGCAAAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCGCTGCCCCAGATCGGCCGC
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTGTCGCTGGTGGCGCGCAACATCAAGACCGAAATCCCCCGCTTCGA
GGATTTGAAGCTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAGCGCGGCCTGGTGCTGTTCGTCGGCGGTACCGGCT
CGGGCAAGTCCACTTCGCTCGCAGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTGCACCGCCACCGCAAGAGCATCATCAACCAGCGCGAGGTGGGGGTGGATACCCGCAGCTTCCACGC
CGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAAATCCGCGATCGCGAGACCATGGAGCATG
CGCTGGCCTTCTCCGAAACCGGCCACCTGGCGATTTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGACCCCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
GCTGGTCAAAACCAGCGACGGTGGTCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATTCGCGACATGATCA
AACGCGGCGAGTTCGGCGGCCTCAAGGAGGTGATGGAGAAATCCAAGGCCCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTAGAGGGGGTGATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGCCAGATCGCGAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAGCCCACCAAGGACG
AAAAAGACGATCTGTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.133

98.945

0.536
  pilU Acinetobacter baylyi ADP1

53.521

93.668

0.501
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.076

93.14

0.383
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Legionella pneumophila strain ERS1305867

41.916

88.127

0.369
  pilT Legionella pneumophila strain Lp02

41.916

88.127

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.116

90.765

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

87.863

0.364
  pilT Vibrio cholerae strain A1552

41.441

87.863

0.364
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361