Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PO845_RS01865 Genome accession   NZ_CP117857
Coordinates   371647..372084 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain PT4     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 366647..377084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PO845_RS01840 (PO845_01840) - 367667..368803 (+) 1137 WP_025284207.1 zinc-dependent alcohol dehydrogenase -
  PO845_RS01845 (PO845_01845) - 368818..369252 (+) 435 WP_007409358.1 RDD family protein -
  PO845_RS01850 (PO845_01850) - 369324..369647 (+) 324 WP_007409357.1 YckD family protein -
  PO845_RS01855 (PO845_01855) - 369751..371187 (+) 1437 WP_015416781.1 family 1 glycosylhydrolase -
  PO845_RS01860 (PO845_01860) nin/comJ 371228..371626 (-) 399 WP_039252825.1 competence protein ComJ Regulator
  PO845_RS01865 (PO845_01865) nucA/comI 371647..372084 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  PO845_RS01870 (PO845_01870) hxlB 372442..372999 (-) 558 WP_039252823.1 6-phospho-3-hexuloisomerase -
  PO845_RS01875 (PO845_01875) hxlA 372996..373631 (-) 636 WP_015416785.1 3-hexulose-6-phosphate synthase -
  PO845_RS01880 (PO845_01880) - 373863..374225 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=788010 PO845_RS01865 WP_003156588.1 371647..372084(-) (nucA/comI) [Bacillus velezensis strain PT4]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=788010 PO845_RS01865 WP_003156588.1 371647..372084(-) (nucA/comI) [Bacillus velezensis strain PT4]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACATCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641