Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Q5420_RS24405 Genome accession   NZ_CP130751
Coordinates   5245627..5247120 (+) Length   497 a.a.
NCBI ID   WP_061189851.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5240627..5252120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5420_RS24375 (Q5420_24375) - 5240838..5241647 (-) 810 WP_003094343.1 ABC transporter ATP-binding protein -
  Q5420_RS24380 (Q5420_24380) kdsD 5242061..5243041 (+) 981 WP_174250742.1 arabinose-5-phosphate isomerase KdsD -
  Q5420_RS24385 (Q5420_24385) - 5243041..5243580 (+) 540 WP_003098849.1 HAD family hydrolase -
  Q5420_RS24390 (Q5420_24390) lptC 5243589..5244161 (+) 573 WP_003094353.1 LPS export ABC transporter periplasmic protein LptC -
  Q5420_RS24395 (Q5420_24395) lptA 5244148..5244675 (+) 528 WP_003120916.1 lipopolysaccharide transport periplasmic protein LptA -
  Q5420_RS24400 (Q5420_24400) lptB 5244675..5245400 (+) 726 WP_003094355.1 LPS export ABC transporter ATP-binding protein -
  Q5420_RS24405 (Q5420_24405) rpoN/rpoN1 5245627..5247120 (+) 1494 WP_061189851.1 RNA polymerase factor sigma-54 Machinery gene
  Q5420_RS24410 (Q5420_24410) hpf 5247198..5247506 (+) 309 WP_003094359.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  Q5420_RS24415 (Q5420_24415) ptsN 5247520..5247984 (+) 465 WP_003098853.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Q5420_RS24420 (Q5420_24420) rapZ 5247986..5248846 (+) 861 WP_003098856.1 RNase adapter RapZ -
  Q5420_RS24425 (Q5420_24425) - 5248876..5249148 (+) 273 WP_003094362.1 HPr family phosphocarrier protein -
  Q5420_RS24430 (Q5420_24430) - 5249245..5250177 (-) 933 WP_003120918.1 ZIP family metal transporter -
  Q5420_RS24435 (Q5420_24435) - 5250196..5250807 (-) 612 WP_061189852.1 superoxide dismutase -
  Q5420_RS24440 (Q5420_24440) - 5250820..5251268 (-) 449 Protein_4834 hypothetical protein -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 56123.31 Da        Isoelectric Point: 4.4593

>NTDB_id=787764 Q5420_RS24405 WP_061189851.1 5245627..5247120(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
MKPSLVLKMGQQLTMTPQLQQAIRLLQLSTLDLQQEIQEALESNPMLERQEDGDDFDNSDPLADGAEQAASAPQESPLQE
SATPSVESLDDDQWSERIPSELPVDTAWEDIYQTSASSLPSNDDDEWDFTARTSSGESLHSHLLWQVNLAPMSDTDRMIA
VTIIDSINNDGYLEESLEEILAAIDPELDVELDEVEVVLRRIQQLEPAGIGARNLRECLLLQLRQLPSTTPWLNEALRLV
SDYLDLLGGRDYSQLMRRMKLKEDELRQVIELIQRLHPRPGSQIESSEAEYIVLDVIVRKDNERWLVELNQEAMPRLRVN
ATYAGMVRRADSSADNTFMRNQLQEARWFIKSLQSRNETLMKVATQIVEHQRGFLDYGEEAMKPLVLHDIAEAVGMHEST
ISRVTTQKYMHTPRGIFELKYFFSSHVSTAEGGECSSTAIRAIIKKLVAAENAKKPLSDSKIAGLLEAQGIQVARRTVAK
YRESLGIAPSSERKRLV

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=787764 Q5420_RS24405 WP_061189851.1 5245627..5247120(+) (rpoN/rpoN1) [Pseudomonas aeruginosa strain AU10241 isolate AU10241_L13_Control]
ATGAAACCATCGCTAGTCCTCAAGATGGGCCAGCAGCTGACGATGACGCCGCAGCTGCAACAGGCCATACGCCTCCTCCA
GCTATCCACCCTGGACCTCCAGCAGGAAATCCAGGAAGCGCTGGAATCCAATCCCATGCTCGAACGCCAGGAAGACGGCG
ACGATTTCGACAACAGCGACCCTCTCGCCGATGGCGCCGAACAGGCGGCCAGCGCGCCCCAGGAGAGCCCTCTCCAGGAA
AGCGCCACGCCCAGCGTCGAAAGCCTCGACGACGACCAGTGGAGCGAACGCATACCCAGCGAGTTGCCGGTGGATACCGC
CTGGGAAGACATCTACCAGACCAGCGCCAGCAGCCTGCCCAGCAACGACGATGACGAGTGGGACTTCACCGCGCGCACCA
GCAGCGGCGAGAGCCTGCACAGCCACCTGCTCTGGCAGGTCAACCTGGCACCGATGTCGGACACCGATCGGATGATCGCG
GTGACCATCATCGATAGCATCAACAACGATGGCTATCTCGAAGAGTCCCTCGAAGAAATCCTCGCCGCCATCGATCCGGA
ACTGGACGTCGAGCTCGACGAAGTGGAAGTCGTGCTGCGCCGCATCCAGCAACTGGAACCCGCCGGCATCGGCGCCCGCA
ACCTGCGCGAGTGCCTGCTGCTGCAACTGCGCCAGTTGCCGTCCACCACGCCCTGGCTGAACGAAGCGCTGCGCCTGGTA
TCCGACTACCTCGACCTGCTCGGCGGCCGCGACTACAGCCAATTGATGCGGCGCATGAAGCTCAAGGAAGACGAACTGCG
CCAGGTCATCGAGCTGATCCAGCGCCTGCATCCCCGGCCCGGTTCGCAGATCGAATCGAGCGAAGCCGAATACATCGTCC
TCGACGTCATCGTGCGCAAGGACAACGAGCGCTGGCTGGTCGAGCTGAACCAGGAGGCGATGCCACGCCTGCGCGTCAAC
GCCACCTACGCCGGCATGGTCCGCCGCGCCGACAGCAGCGCCGACAATACCTTCATGCGCAACCAGTTGCAGGAAGCCCG
CTGGTTCATCAAGAGCCTGCAGAGCCGCAACGAAACGCTGATGAAGGTCGCCACCCAGATCGTCGAGCACCAGCGCGGTT
TCCTCGACTACGGCGAAGAAGCCATGAAGCCGCTGGTCCTGCACGACATCGCCGAAGCGGTGGGCATGCACGAATCGACC
ATTTCGCGGGTCACCACGCAGAAATACATGCATACGCCGCGCGGCATTTTCGAGCTGAAATATTTCTTTTCAAGTCACGT
CAGTACCGCCGAAGGCGGGGAATGCTCGTCCACCGCGATCCGCGCGATCATCAAGAAACTGGTGGCCGCGGAAAATGCGA
AAAAGCCATTGAGCGACAGCAAGATCGCTGGTTTACTGGAGGCACAGGGCATTCAAGTGGCTCGCCGCACCGTCGCCAAA
TACCGCGAATCCCTGGGCATAGCCCCTTCGAGCGAGCGCAAGCGACTGGTGTGA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(129-323)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(337-495)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.457

100

0.475