Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   Bsp3421_RS27635 Genome accession   NZ_CP117782
Coordinates   2587029..2588534 (+) Length   501 a.a.
NCBI ID   WP_274004380.1    Uniprot ID   -
Organism   Burkholderia sp. FERM BP-3421     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582029..2593534
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Bsp3421_RS27610 (Bsp3421_005522) kdsD 2583282..2584265 (+) 984 WP_273999178.1 arabinose 5-phosphate isomerase KdsD -
  Bsp3421_RS27615 (Bsp3421_005523) - 2584265..2584804 (+) 540 WP_273999180.1 HAD hydrolase family protein -
  Bsp3421_RS27620 (Bsp3421_005524) lptC 2584808..2585422 (+) 615 WP_273999181.1 LPS export ABC transporter periplasmic protein LptC -
  Bsp3421_RS27625 (Bsp3421_005525) lptA 2585441..2586109 (+) 669 WP_273999182.1 lipopolysaccharide transport periplasmic protein LptA -
  Bsp3421_RS27630 (Bsp3421_005526) lptB 2586106..2586882 (+) 777 WP_273999183.1 LPS export ABC transporter ATP-binding protein -
  Bsp3421_RS27635 (Bsp3421_005527) rpoN/rpoN1 2587029..2588534 (+) 1506 WP_274004380.1 RNA polymerase factor sigma-54 Machinery gene
  Bsp3421_RS27640 (Bsp3421_005528) raiA 2588693..2589052 (+) 360 WP_273999185.1 ribosome-associated translation inhibitor RaiA -
  Bsp3421_RS27645 (Bsp3421_005529) ptsN 2589263..2589775 (+) 513 WP_252983355.1 PTS IIA-like nitrogen regulatory protein PtsN -
  Bsp3421_RS27650 (Bsp3421_005530) hprK 2589903..2590871 (+) 969 WP_252983354.1 HPr(Ser) kinase/phosphatase -
  Bsp3421_RS27655 (Bsp3421_005531) rapZ 2590948..2591841 (+) 894 WP_273999188.1 RNase adapter RapZ -
  Bsp3421_RS27660 (Bsp3421_005532) - 2591870..2592505 (+) 636 WP_273999189.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55159.21 Da        Isoelectric Point: 4.9376

>NTDB_id=787609 Bsp3421_RS27635 WP_274004380.1 2587029..2588534(+) (rpoN/rpoN1) [Burkholderia sp. FERM BP-3421]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVSQNPLLENDDDWITSPLRVASDGSLIAQSPAQASADMSG
QPPNGSGASNERPERDEPAGADEYDGYSDNGDASPWNLDDFGRSAPSSDEDDIPPLQVHEATTSLRDHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLTASFDEILADLPAELEIDVDELNAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRVL
ALEIVSQYLELLAARDFTRLRKHLKANDDELRDAHTLIRSLEPFPGSAYGKAEADYVVPDIIVKKSGQSWHAELNPEVVP
RLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=787609 Bsp3421_RS27635 WP_274004380.1 2587029..2588534(+) (rpoN/rpoN1) [Burkholderia sp. FERM BP-3421]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAACATCTCGCGCTGACCCCGCAGCTGCAGCAGTCGATCCGCCTGCTGCA
GCTCTCGACGCTCGAATTGCAGCAGGAAGTGGCGATGGCGGTCTCACAGAACCCCCTGCTCGAGAACGACGACGACTGGA
TCACGAGCCCGCTGCGCGTCGCGTCGGACGGCTCGCTGATCGCGCAGTCGCCTGCGCAGGCATCGGCCGACATGTCGGGG
CAGCCGCCGAACGGTTCGGGCGCGTCCAATGAGCGCCCGGAACGCGACGAGCCCGCCGGTGCCGACGAATACGACGGCTA
CTCCGACAACGGCGACGCGAGCCCGTGGAATCTCGACGACTTCGGCCGCTCCGCCCCCTCGTCCGATGAAGACGACATAC
CGCCGCTGCAGGTCCATGAAGCCACGACCTCGCTGCGCGATCACCTGTCCGCGCAGTTGCGCGTGACGCAGGCCGGCCCG
CGCGACCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTCACCGCGTCGTTCGACGAGATCCT
GGCCGACCTGCCCGCCGAACTCGAGATCGACGTCGACGAACTGAACGCCGCGCTCGCGCTGCTGCACAGCTTCGACCCAG
CAGGCGTCGGCGCGCGCTCGGCCTCCGAATGCCTGAGGCTGCAGCTGCTGCGGCTGGATCCGTCCCCGACGCGCGTGCTC
GCGCTCGAGATCGTGTCGCAATATCTGGAGCTGCTGGCTGCGCGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAA
CGACGACGAGCTGCGCGACGCGCACACGCTGATCCGCTCGCTCGAACCGTTCCCCGGCTCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTGCCCGACATCATCGTGAAGAAAAGCGGCCAGAGCTGGCACGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGCATCAACCATCTTTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGAGCGCCGGATCGTTGAAGCAGCA
ACTGCAGGAAGCGCGTTGGCTCATCAAGAATATCCAGCAGCGCTTCGACACGATCCTGCGCGTCGCGCAGGCGATTGTCG
AACGTCAGAAGAATTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACAACAGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCACACGTGTCGACGGATACAGGGGGCGCCGCGTCATCCACGGCGATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCCAAAATCTCCTCTTTCGGACAGCCGCATAGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCCAAGTATCGCGAAGCCCTCAAGATCCCAGCGGTGAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.787

100

0.691