Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PSR14_RS20965 Genome accession   NZ_CP117702
Coordinates   3981453..3981887 (-) Length   144 a.a.
NCBI ID   WP_000811499.1    Uniprot ID   A0A1J9ZQC5
Organism   Bacillus sp. A01H     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3976453..3986887
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR14_RS20945 (PSR14_20935) - 3976528..3977316 (+) 789 WP_073536030.1 RNA-guided endonuclease TnpB family protein -
  PSR14_RS20950 (PSR14_20940) - 3977476..3978033 (+) 558 WP_000062073.1 PadR family transcriptional regulator -
  PSR14_RS20955 (PSR14_20945) metE 3978701..3980989 (+) 2289 WP_001007595.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  PSR14_RS20960 (PSR14_20950) comJ 3981027..3981416 (-) 390 WP_000424017.1 competence protein ComJ -
  PSR14_RS20965 (PSR14_20955) nucA/comI 3981453..3981887 (-) 435 WP_000811499.1 DNA-entry nuclease Machinery gene
  PSR14_RS20970 (PSR14_20960) - 3982124..3982873 (+) 750 WP_098487380.1 DUF3967 domain-containing protein -
  PSR14_RS20975 (PSR14_20965) kinB 3982927..3984201 (-) 1275 WP_000420384.1 sporulation sensor histidine kinase KinB -
  PSR14_RS20980 (PSR14_20970) - 3984511..3984711 (+) 201 WP_000929285.1 hypothetical protein -
  PSR14_RS20985 (PSR14_20975) - 3984835..3985998 (+) 1164 WP_000434564.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15996.35 Da        Isoelectric Point: 8.4331

>NTDB_id=787139 PSR14_RS20965 WP_000811499.1 3981453..3981887(-) (nucA/comI) [Bacillus sp. A01H]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQVLEFPKDRYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=787139 PSR14_RS20965 WP_000811499.1 3981453..3981887(-) (nucA/comI) [Bacillus sp. A01H]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTGTATGAAGTGCTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCGTATGATCAAGTTCTAGAATTTCCGAAAGATCGATACCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTGTGTACAATCGACCGTGGTGGCGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCGCACATCGAATATATAAGTCCAGCAGATAATCGCGGAGCAGGCTCTTGGGTAGGAAATAAGTTAGATAAATACC
CAGATGGTACGCGTGTAAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1J9ZQC5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

72.072

77.083

0.556