Detailed information NTG0787122

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: PSR14_RS06585
• Genome accession: NZ_CP117702
• Coordinates: 1243089..1246814 (+)
• Length: 1241 a.a.
• NCBI ID: WP_086405318.1
• Uniprot ID: -
• Organism: Bacillus sp. A01H
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1238089..1251814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSR14_RS06570 (PSR14_06575)	-	1238247..1238840 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
PSR14_RS06575 (PSR14_06580)	lepB	1238897..1239460 (+)	564	WP_000751900.1	signal peptidase I	-
PSR14_RS06580 (PSR14_06585)	addB	1239577..1243092 (+)	3516	WP_098487486.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
PSR14_RS06585 (PSR14_06590)	addA	1243089..1246814 (+)	3726	WP_086405318.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
PSR14_RS06590 (PSR14_06595)	-	1246827..1247114 (+)	288	WP_000255720.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
PSR14_RS06595 (PSR14_06600)	gerPF	1247219..1247434 (-)	216	WP_001141566.1	spore germination protein GerPF	-
PSR14_RS06600 (PSR14_06605)	-	1247477..1247863 (-)	387	WP_000902335.1	spore germination protein GerPE	-
PSR14_RS06605 (PSR14_06610)	gerPD	1247879..1248073 (-)	195	WP_001052802.1	spore germination protein GerPD	-
PSR14_RS06610 (PSR14_06615)	gerPC	1248080..1248694 (-)	615	WP_001070747.1	spore germination protein GerPC	-
PSR14_RS06615 (PSR14_06620)	gerPB	1248762..1248968 (-)	207	WP_001012508.1	spore germination protein GerPB	-
PSR14_RS06620 (PSR14_06625)	gerPA	1248983..1249204 (-)	222	WP_001111188.1	spore germination protein GerPA	-
PSR14_RS06625 (PSR14_06630)	-	1249301..1249480 (-)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
PSR14_RS06630 (PSR14_06635)	-	1249725..1250627 (+)	903	WP_000945298.1	fumarylacetoacetate hydrolase family protein	-
PSR14_RS06635 (PSR14_06640)	-	1250664..1250948 (-)	285	WP_000926869.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142757.03 Da
• Isoelectric Point: 4.8519

>NTDB_id=787122 PSR14_RS06585 WP_086405318.1 1243089..1246814(+) (addA) [Bacillus sp. A01H]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSVPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITEEVIREQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGQSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFDQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3726 bp

>NTDB_id=787122 PSR14_RS06585 WP_086405318.1 1243089..1246814(+) (addA) [Bacillus sp. A01H]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTCATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTGTTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGGATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCCTACTTATTAGAAGATGTGAAATTTCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAGTCATGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAACGAGGATGTTGTAAAACAAGTAGACTCCCTTCGTAATAAAGCGAAAGATGAAGTG
AAAAAATTACAAGAAGAGCTATTTAGCCGTAAGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACAGAACGTTTCCAAGCGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGGAAGTATAAACGTTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACAAACTTTATTTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGTTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAAGCACAGCTTGAAGCACGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGCGACTTCGTTATTTTACTTCGCTCCATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCATGGGAAGAAAGGATCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGGGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGAGTGGTTCTATAACTTATTACAAGGATGGCGTGAATTTGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTTCCAGTCGTATTTGTCGCTGGACTTGGTCGTCGCTTTAATACACAAGACTTAATGAA
GCGTTTCTTACTGCATAAAGACTTCGGTTTTGGTTCGCAATTTATCGATCCTCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATCGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGAGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCATTATATAGGC
ACCGTGATAGTGAAATACTTCTTGAATTAGGACAAGGAAGTGTTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGTAACACGTTACTCGCGCCAGAACCGGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCCCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGGACACGTCCTCGTTTTATGGAGAAAAAGGGATTAACATATGCAGAGCGCGG
AACAGCAGTACATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGAAGAAGTGATTCGGGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTCGAGCAGGCGGAAGAAATTGCGATTGAAAAAGTAATTTCATTCTTTGATAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGCAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATTGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCAGGTGGATTTGATCAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTCCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1232)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.666	100	0.537