Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PSR56_RS22665 Genome accession   NZ_CP117689
Coordinates   4311032..4312084 (-) Length   350 a.a.
NCBI ID   WP_028411611.1    Uniprot ID   -
Organism   Priestia megaterium strain GEB3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4306032..4317084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR56_RS22625 (PSR56_22625) - 4307048..4307872 (+) 825 WP_048019760.1 YqhG family protein -
  PSR56_RS22630 (PSR56_22630) - 4307896..4308081 (-) 186 WP_013059170.1 YqzE family protein -
  PSR56_RS22635 (PSR56_22635) comGG 4308125..4308499 (-) 375 WP_033579700.1 competence type IV pilus minor pilin ComGG -
  PSR56_RS22640 (PSR56_22640) comGF 4308489..4308926 (-) 438 WP_048019759.1 competence type IV pilus minor pilin ComGF -
  PSR56_RS22645 (PSR56_22645) - 4308923..4309261 (-) 339 WP_033579698.1 type II secretion system protein -
  PSR56_RS22650 (PSR56_22650) comGD 4309245..4309682 (-) 438 WP_033579697.1 competence type IV pilus minor pilin ComGD -
  PSR56_RS22655 (PSR56_22655) comGC 4309669..4309980 (-) 312 WP_049166200.1 competence type IV pilus major pilin ComGC -
  PSR56_RS22660 (PSR56_22660) comGB 4309995..4311041 (-) 1047 WP_033579696.1 competence type IV pilus assembly protein ComGB -
  PSR56_RS22665 (PSR56_22665) comGA 4311032..4312084 (-) 1053 WP_028411611.1 competence type IV pilus ATPase ComGA Machinery gene
  PSR56_RS22670 (PSR56_22670) - 4312353..4312736 (-) 384 WP_028411612.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PSR56_RS22675 (PSR56_22675) - 4312998..4313234 (+) 237 WP_013059179.1 DUF2626 domain-containing protein -
  PSR56_RS22680 (PSR56_22680) - 4313272..4313904 (-) 633 WP_310884104.1 MBL fold metallo-hydrolase -
  PSR56_RS22685 (PSR56_22685) - 4314114..4314290 (+) 177 WP_028411614.1 DUF2759 domain-containing protein -
  PSR56_RS22690 (PSR56_22690) - 4314326..4315228 (-) 903 WP_243495162.1 M14 family metallocarboxypeptidase -
  PSR56_RS22695 (PSR56_22695) - 4315340..4316308 (-) 969 WP_013059183.1 ROK family glucokinase -
  PSR56_RS22700 (PSR56_22700) - 4316311..4316517 (-) 207 WP_028411617.1 YqgQ family protein -

Sequence

Protein


Download         Length: 350 a.a.        Molecular weight: 39596.97 Da        Isoelectric Point: 9.0586

>NTDB_id=786998 PSR56_RS22665 WP_028411611.1 4311032..4312084(-) (comGA) [Priestia megaterium strain GEB3]
MTEIEQFATYVIRDACECRSSDVHIVPRKKDAVIQLRIDQELILKRVISKEMSERLISHFKFLGGMDIGERRKPQNGALT
TSVDHYQVNLRLSTLPTSHDESLVIRLLPHQQSTSLKHLSLFPEHTKKLISLLKFSHGLIIFTGPTGSGKTTTLYTLLHE
VKHLFNRNVITLEDPIEKPSETVLQVQVNERAGITYTTGLKAILRHDPDIIMVGEIRDSETAKIAVRAALTGHLVLTTMH
TKDTKGSLYRLLEFGVSFQEMEQTLVAVAAQRLVEIRCPLCSGKCHPACKKMRKHRQSSIYELLYGKELSAVMREVKGEN
ADYDYKTLRDVILKGIALGYLYPHSLDGWG

Nucleotide


Download         Length: 1053 bp        

>NTDB_id=786998 PSR56_RS22665 WP_028411611.1 4311032..4312084(-) (comGA) [Priestia megaterium strain GEB3]
TTGACCGAAATTGAACAGTTTGCTACTTACGTTATACGAGATGCATGTGAGTGTCGAAGTTCTGATGTGCATATTGTGCC
CCGAAAGAAAGATGCTGTGATTCAGCTGCGAATTGACCAGGAGCTTATTTTAAAGAGGGTTATTTCAAAGGAAATGTCTG
AACGGCTCATTTCGCATTTTAAATTTTTAGGGGGAATGGATATTGGGGAGCGCAGAAAACCTCAAAATGGTGCATTGACC
ACTTCCGTTGATCACTATCAAGTAAATTTGCGTTTATCAACTCTTCCTACTTCTCATGATGAAAGTCTTGTTATTCGGCT
TTTGCCTCATCAACAATCTACCTCTCTCAAACATTTATCTCTTTTTCCTGAACATACTAAAAAGCTTATTTCTCTCTTGA
AATTCTCTCATGGATTAATTATTTTTACCGGTCCTACAGGATCAGGTAAAACCACTACTCTATATACTCTTTTACACGAA
GTAAAGCACCTTTTTAATCGAAATGTTATTACACTAGAAGATCCTATTGAAAAACCGAGTGAAACCGTGCTTCAAGTGCA
GGTAAATGAACGCGCAGGGATTACGTATACGACTGGTTTAAAAGCTATATTGCGCCATGATCCAGACATTATCATGGTGG
GAGAAATAAGAGATAGTGAGACCGCCAAAATTGCTGTGAGAGCAGCGCTTACGGGACACTTGGTTTTGACAACGATGCAT
ACTAAAGATACAAAAGGATCTTTGTACAGGCTGCTTGAGTTTGGCGTTAGCTTTCAAGAAATGGAGCAGACTCTTGTAGC
AGTAGCTGCACAGCGTCTTGTAGAAATTCGCTGTCCGCTGTGTTCGGGAAAATGCCACCCTGCTTGTAAAAAAATGAGGA
AGCATCGTCAGTCCAGTATATACGAATTGCTTTATGGCAAGGAGCTTAGCGCAGTGATGAGAGAAGTGAAAGGAGAAAAT
GCGGATTATGATTATAAGACGCTCCGAGATGTTATTCTAAAAGGAATTGCGCTAGGCTATTTATATCCGCATTCGCTAGA
CGGATGGGGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

58.571

100

0.586