Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   PSR56_RS16745 Genome accession   NZ_CP117689
Coordinates   3221070..3221441 (+) Length   123 a.a.
NCBI ID   WP_372452795.1    Uniprot ID   -
Organism   Priestia megaterium strain GEB3     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3216070..3226441
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSR56_RS16720 (PSR56_16720) - 3216371..3217204 (-) 834 WP_048018915.1 AraC family transcriptional regulator -
  PSR56_RS16725 (PSR56_16725) - 3217407..3218228 (+) 822 WP_028414492.1 sensor histidine kinase -
  PSR56_RS16730 (PSR56_16730) - 3218229..3218870 (+) 642 WP_033580507.1 response regulator transcription factor -
  PSR56_RS16735 (PSR56_16735) - 3219005..3220384 (+) 1380 WP_255901645.1 PepSY domain-containing protein -
  PSR56_RS16740 (PSR56_16740) - 3220414..3220878 (+) 465 WP_255901648.1 FixH family protein -
  PSR56_RS16745 (PSR56_16745) nucA/comI 3221070..3221441 (+) 372 WP_372452795.1 DNA-entry nuclease Machinery gene
  PSR56_RS16750 (PSR56_16750) - 3221596..3221949 (+) 354 WP_223272376.1 DUF1516 family protein -
  PSR56_RS16755 (PSR56_16755) - 3221989..3222582 (-) 594 WP_028414498.1 hypothetical protein -
  PSR56_RS16760 (PSR56_16760) - 3222735..3223391 (-) 657 WP_048019653.1 TetR/AcrR family transcriptional regulator -
  PSR56_RS16765 (PSR56_16765) - 3223414..3224691 (-) 1278 WP_048019607.1 glycosyltransferase -
  PSR56_RS16770 (PSR56_16770) - 3224991..3225536 (+) 546 WP_048019606.1 GNAT family N-acetyltransferase -
  PSR56_RS16775 (PSR56_16775) - 3225667..3226152 (-) 486 WP_168761023.1 hypothetical protein -

Sequence

Protein


Download         Length: 123 a.a.        Molecular weight: 13464.68 Da        Isoelectric Point: 4.2461

>NTDB_id=786985 PSR56_RS16745 WP_372452795.1 3221070..3221441(+) (nucA/comI) [Priestia megaterium strain GEB3]
MIEGNPLEQNSSTNPSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRSESLKGIPTKSGYDRDEFPMAF
CEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 372 bp        

>NTDB_id=786985 PSR56_RS16745 WP_372452795.1 3221070..3221441(+) (nucA/comI) [Priestia megaterium strain GEB3]
ATGATTGAAGGAAATCCGTTAGAGCAGAACTCTTCAACGAATCCCTCCTACGATGAAGTCATTACCTTCCCTGCTGATCG
TTACCCTGAAACTGCAGCGCACATTCAACATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATCGATCGAGACGGTG
CTGAAGAAAACCGCAGTGAGTCTTTAAAAGGCATTCCGACTAAAAGCGGCTATGATCGAGATGAGTTTCCGATGGCCTTT
TGTGAAGAAGGCGGAAGCGGCGCAGATATTGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGGTCTTGGATTTCACA
TCAAGTAGATGAGTTTCCAGACGGTACGAAGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(26-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.4

100

0.593