Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   Q5R37_RS21415 Genome accession   NZ_CP130706
Coordinates   4408939..4409616 (+) Length   225 a.a.
NCBI ID   WP_000178161.1    Uniprot ID   -
Organism   Escherichia coli strain DDG220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4403939..4414616
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q5R37_RS21380 (Q5R37_21380) gspG 4404419..4404856 (+) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  Q5R37_RS21385 (Q5R37_21385) gspH 4404864..4405373 (+) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  Q5R37_RS21390 (Q5R37_21390) gspI 4405370..4405747 (+) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  Q5R37_RS21395 (Q5R37_21395) gspJ 4405740..4406327 (+) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  Q5R37_RS21400 (Q5R37_21400) gspK 4406320..4407303 (+) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  Q5R37_RS21405 (Q5R37_21405) gspL 4407318..4408481 (+) 1164 WP_001115238.1 type II secretion system protein GspL -
  Q5R37_RS21410 (Q5R37_21410) gspM 4408478..4408939 (+) 462 WP_001295161.1 type II secretion system protein GspM -
  Q5R37_RS21415 (Q5R37_21415) pilD 4408939..4409616 (+) 678 WP_000178161.1 prepilin peptidase GspO Machinery gene
  Q5R37_RS21420 (Q5R37_21420) bfr 4409645..4410121 (-) 477 WP_000675504.1 bacterioferritin -
  Q5R37_RS21425 (Q5R37_21425) bfd 4410193..4410387 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  Q5R37_RS21430 (Q5R37_21430) chiA 4410556..4413249 (-) 2694 WP_000773156.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24898.78 Da        Isoelectric Point: 7.9563

>NTDB_id=786730 Q5R37_RS21415 WP_000178161.1 4408939..4409616(+) (pilD) [Escherichia coli strain DDG220]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=786730 Q5R37_RS21415 WP_000178161.1 4408939..4409616(+) (pilD) [Escherichia coli strain DDG220]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378