Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   GsuE55_RS14795 Genome accession   NZ_AP022557
Coordinates   2986063..2987433 (+) Length   456 a.a.
NCBI ID   WP_033842702.1    Uniprot ID   -
Organism   Geobacillus subterraneus strain E55-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2981063..2992433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GsuE55_RS14775 (GsuE55_29630) - 2981430..2981891 (+) 462 WP_033021074.1 CtsR family transcriptional regulator -
  GsuE55_RS14780 (GsuE55_29640) - 2981907..2982455 (+) 549 WP_033021072.1 UvrB/UvrC motif-containing protein -
  GsuE55_RS14785 (GsuE55_29650) - 2982460..2983551 (+) 1092 WP_061912144.1 protein arginine kinase -
  GsuE55_RS14790 (GsuE55_29660) clpC 2983548..2985983 (+) 2436 WP_033021066.1 ATP-dependent protease ATP-binding subunit ClpC -
  GsuE55_RS14795 (GsuE55_29670) radA 2986063..2987433 (+) 1371 WP_033842702.1 DNA repair protein RadA Machinery gene
  GsuE55_RS14800 (GsuE55_29680) - 2987620..2988714 (+) 1095 WP_033842700.1 PIN/TRAM domain-containing protein -
  GsuE55_RS14805 (GsuE55_29690) ispD 2988735..2989421 (+) 687 WP_033842699.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  GsuE55_RS14810 (GsuE55_29700) ispF 2989437..2989919 (+) 483 WP_172418727.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  GsuE55_RS14815 (GsuE55_29710) gltX 2990419..2991891 (+) 1473 WP_033842698.1 glutamate--tRNA ligase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49411.90 Da        Isoelectric Point: 7.7489

>NTDB_id=78635 GsuE55_RS14795 WP_033842702.1 2986063..2987433(+) (radA) [Geobacillus subterraneus strain E55-1]
MVKKKTKFVCQECGYESAKWLGRCPGCQTWNSFVEEIERAVSGRGAFLHTEPSSSAQPVPITAVTATQEPRIRTNSAELD
RVLGGGIVKGSLVLIGGDPGIGKSTLLLQTSAQLAAARHKVLYISGEESVKQVKLRAGRLHAESNELYVLAEANMEYIVT
AVETIAPACVIIDSIQTVYRTDITSAPGSVAQVRECTAELMKIAKTKGIAIFIVGHVTKEGAIAGPRLLEHMVDTVLYFE
GERHHTYRILRAVKNRFGSTNEIGIFEMRDIGLREVENPSEVFLEERSRGAAGSTVVAAMEGTRPVLVEIQALVSPTSFG
NPRRMATGLDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAVAVSIASSFRDRPTHPADVIIGEVGLTG
EVRRVSRIEQRVQEAAKLGFSRVIVPKNNITGWQPPDGIQVIGVSHVAEALEHTMV

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=78635 GsuE55_RS14795 WP_033842702.1 2986063..2987433(+) (radA) [Geobacillus subterraneus strain E55-1]
ATGGTCAAAAAGAAAACGAAGTTTGTTTGTCAAGAGTGCGGCTATGAGTCGGCCAAATGGCTCGGCCGCTGTCCAGGATG
CCAAACGTGGAATTCGTTTGTGGAGGAAATCGAGCGGGCGGTTTCTGGGCGTGGCGCGTTTCTTCACACTGAACCGTCCA
GTTCGGCCCAACCGGTTCCGATTACGGCGGTCACAGCTACGCAAGAACCGCGCATCCGAACGAACAGCGCGGAACTCGAT
CGCGTGCTCGGCGGCGGGATCGTCAAAGGTTCGCTCGTCTTGATCGGCGGTGACCCCGGGATTGGCAAATCGACGTTGCT
GCTGCAGACGTCGGCCCAGCTCGCCGCGGCCCGTCACAAGGTGTTGTATATATCCGGGGAGGAATCAGTGAAGCAGGTGA
AGCTGCGCGCCGGGCGCCTCCACGCCGAATCAAATGAACTATATGTGTTAGCCGAAGCGAATATGGAATATATTGTAACA
GCGGTGGAAACGATTGCGCCTGCCTGTGTGATCATCGATTCCATTCAGACGGTGTACCGAACCGATATTACGTCAGCCCC
GGGCAGCGTCGCCCAAGTGCGCGAATGTACGGCCGAGCTGATGAAAATCGCCAAAACGAAAGGCATCGCCATTTTCATTG
TCGGCCATGTGACGAAAGAAGGGGCGATCGCCGGGCCGCGCTTGCTTGAACATATGGTTGATACGGTTCTTTATTTTGAA
GGGGAGCGGCATCATACGTACCGTATTTTGCGGGCGGTGAAAAACCGCTTCGGTTCGACGAATGAAATCGGCATTTTTGA
AATGCGTGACATCGGGCTGCGAGAGGTGGAAAACCCGTCGGAAGTGTTTTTGGAAGAGCGGTCGCGCGGAGCGGCGGGCT
CCACGGTCGTCGCGGCCATGGAAGGGACGCGCCCGGTGCTCGTTGAAATTCAGGCGCTCGTTTCGCCGACGAGTTTTGGC
AACCCAAGGCGAATGGCGACCGGCCTCGATCACAACCGCGTTTCGCTGCTGATGGCGGTGCTTGAAAAGCGGGTCGGGCT
GCTGTTGCAAAACCAAGACGCCTACTTGAAGGTAGCGGGCGGGGTAAAGCTTGACGAACCGGCCATTGACTTGGCGGTCG
CCGTCAGCATCGCCTCAAGTTTTCGCGACCGGCCGACCCATCCGGCCGATGTGATCATCGGCGAGGTTGGCTTGACCGGC
GAGGTGCGCCGTGTCTCGCGCATTGAACAACGGGTGCAAGAGGCGGCCAAACTAGGGTTTTCGCGCGTCATTGTGCCAAA
AAACAATATCACGGGCTGGCAGCCCCCCGACGGCATCCAAGTAATCGGCGTATCACATGTCGCTGAGGCCCTCGAGCATA
CGATGGTGTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(364-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

73.246

100

0.732
  radA Streptococcus mitis NCTC 12261

65.121

99.342

0.647
  radA Streptococcus mitis SK321

65.044

99.123

0.645
  radA Streptococcus pneumoniae Rx1

64.823

99.123

0.643
  radA Streptococcus pneumoniae D39

64.823

99.123

0.643
  radA Streptococcus pneumoniae R6

64.823

99.123

0.643
  radA Streptococcus pneumoniae TIGR4

64.823

99.123

0.643

Multiple sequence alignment