Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   PGW99_RS02245 Genome accession   NZ_CP117520
Coordinates   441834..442595 (+) Length   253 a.a.
NCBI ID   WP_273778481.1    Uniprot ID   -
Organism   Acinetobacter sp. GSS19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 436834..447595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PGW99_RS02215 - 437210..437581 (+) 372 WP_273779406.1 hypothetical protein -
  PGW99_RS02220 - 437713..438999 (+) 1287 WP_273778476.1 glycosyltransferase family 1 protein -
  PGW99_RS02225 - 439030..439581 (+) 552 WP_273778477.1 phosphatase PAP2 family protein -
  PGW99_RS02230 iscX 439669..439869 (+) 201 WP_273778478.1 Fe-S cluster assembly protein IscX -
  PGW99_RS02235 ndk 439977..440408 (+) 432 WP_273778479.1 nucleoside-diphosphate kinase -
  PGW99_RS02240 rlmN 440533..441768 (+) 1236 WP_273778480.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PGW99_RS02245 pilF 441834..442595 (+) 762 WP_273778481.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  PGW99_RS02250 - 442586..443374 (+) 789 WP_273778482.1 RodZ domain-containing protein -
  PGW99_RS02255 ispG 443397..444512 (+) 1116 WP_273778483.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  PGW99_RS02260 hisS 444509..445801 (+) 1293 WP_273778484.1 histidine--tRNA ligase -
  PGW99_RS02265 - 445831..446535 (+) 705 WP_273778485.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 253 a.a.        Molecular weight: 28813.54 Da        Isoelectric Point: 6.9864

>NTDB_id=786218 PGW99_RS02245 WP_273778481.1 441834..442595(+) (pilF) [Acinetobacter sp. GSS19]
MSAFIAQGCQTHTPGMSKDPEKSVKVRTQLAAEYIRKGDLDAAKRSLDQALEVDSRDATANMMMGVLLQQEGSPLNMEKA
DEYFRKSISIDPQNAQARNNYGTYLYQLQRYNEAIEQLSIAGATLGYDQRFRALENLGRIYLQLGDVEKAEKAFKQALQV
NRDSYVSMLELSEIFYLRQQIPAATQLYQQYVTAVGQNNQGARALWLGIRLGRANGEDMNKQVLVNQLRALFPDSQEYQR
YLQLQYSTEAVWK

Nucleotide


Download         Length: 762 bp        

>NTDB_id=786218 PGW99_RS02245 WP_273778481.1 441834..442595(+) (pilF) [Acinetobacter sp. GSS19]
ATGTCGGCATTCATTGCACAAGGTTGCCAGACCCATACTCCGGGTATGAGTAAAGACCCGGAGAAATCGGTTAAGGTGCG
TACTCAATTGGCTGCTGAATATATCCGCAAAGGTGATCTGGATGCAGCAAAACGCTCACTTGATCAGGCACTTGAGGTGG
ATTCGCGTGATGCCACTGCCAATATGATGATGGGGGTGTTATTGCAGCAGGAAGGCAGTCCGCTTAACATGGAAAAAGCG
GATGAGTATTTCCGTAAATCCATTTCGATTGATCCACAAAATGCGCAAGCTCGTAATAATTATGGGACATATTTATATCA
GCTTCAGCGTTATAATGAAGCGATTGAACAGCTCAGTATCGCAGGAGCTACACTCGGATATGATCAGCGTTTCCGTGCCT
TAGAGAATTTGGGACGGATTTATCTGCAACTGGGTGATGTAGAGAAAGCAGAAAAAGCTTTTAAACAAGCATTACAAGTG
AACCGTGACTCTTATGTTTCGATGCTGGAGTTGTCAGAAATTTTTTATTTGCGGCAGCAAATACCGGCAGCTACCCAGCT
CTATCAACAGTACGTCACGGCTGTTGGACAAAATAATCAAGGTGCACGTGCACTCTGGTTAGGTATTCGCCTTGGGCGTG
CCAATGGTGAAGACATGAACAAGCAGGTTCTGGTGAACCAGTTACGTGCTTTGTTCCCGGATAGCCAGGAATATCAACGT
TATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_12)

(95-162)

Tetratricopeptide repeat (TPR_8)

(26-55)

Tetratricopeptide repeat (TPR_8)

(76-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

76.892

99.209

0.763
  pilF Acinetobacter baylyi ADP1

70.356

100

0.704