Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSC74_RS01455 Genome accession   NZ_CP117470
Coordinates   282637..283746 (-) Length   369 a.a.
NCBI ID   WP_273775949.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 516     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 277637..288746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC74_RS01435 (PSC74_01435) brnQ 278095..279402 (-) 1308 WP_011707394.1 branched-chain amino acid transport system II carrier protein -
  PSC74_RS01440 (PSC74_01440) - 279593..280303 (-) 711 WP_135318966.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  PSC74_RS01445 (PSC74_01445) srmB 280432..281655 (+) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  PSC74_RS01450 (PSC74_01450) yaaA 281832..282605 (-) 774 WP_135318967.1 peroxide stress protein YaaA -
  PSC74_RS01455 (PSC74_01455) pilU 282637..283746 (-) 1110 WP_273775949.1 type IVa pilus ATPase TapU Machinery gene
  PSC74_RS01460 (PSC74_01460) pilT 283775..284809 (-) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  PSC74_RS01465 (PSC74_01465) - 284849..285550 (+) 702 WP_135318968.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSC74_RS01470 (PSC74_01470) proC 285609..286433 (+) 825 WP_135318969.1 pyrroline-5-carboxylate reductase -
  PSC74_RS01475 (PSC74_01475) - 286474..287025 (+) 552 WP_016351969.1 YggT family protein -
  PSC74_RS01480 (PSC74_01480) yggU 287025..287324 (+) 300 WP_011707385.1 DUF167 family protein YggU -
  PSC74_RS01485 (PSC74_01485) - 287343..287762 (+) 420 WP_135318971.1 DUF4426 domain-containing protein -
  PSC74_RS01490 (PSC74_01490) - 287817..288128 (-) 312 WP_024945836.1 hypothetical protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41079.22 Da        Isoelectric Point: 6.5035

>NTDB_id=785883 PSC74_RS01455 WP_273775949.1 282637..283746(-) (pilU) [Aeromonas hydrophila strain 516]
MNLDALLTELVTRKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=785883 PSC74_RS01455 WP_273775949.1 282637..283746(-) (pilU) [Aeromonas hydrophila strain 516]
ATGAATCTGGATGCCCTGCTGACCGAGCTGGTCACCCGCAAAGGCTCGGATCTGTTCATCACGGTCGGCACACCGCCGAC
CCTCAAGGTCAACGGCCACCTGCAGTCACTGGCAGAAGCCCCCCTCGACAAGGCCAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTTGAGCGCTACCTGCACACCAAGGAGGCCAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGTTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGCAAGTCCACCACCCAGGCGGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTGGGCATAGACACAGCGTCGTTCGACGT
GGCGCTGAAGAGTTCACTGCGCCAGGCCCCTGACGTGATCCTGATCGGTGAAATCCGCAGCCAGGAGACCATGGAGTTTG
CCCTGCAGTTTGCCGAGACTGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAGCACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGGCGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.87

99.729

0.607
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.994

89.702

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363