Detailed information NTG0785736

Bioinformatically predicted

Overview

• Name: radA
• Type: Machinery gene
• Locus tag: PSH69_RS25860
• Genome accession: NZ_CP117461
• Coordinates: 5758677..5760044 (-)
• Length: 455 a.a.
• NCBI ID: WP_019581287.1
• Uniprot ID: A0AB33E587
• Organism: Pseudomonas sp. FP1740
• Function: homologous recombination (predicted from homology) Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5751300..5775063	5758677..5760044	within	0

Gene organization within MGE regions

Location: 5751300..5775063

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH69_RS25830 (PSH69_25820)	-	5751300..5751452 (+)	153	WP_019581282.1	hypothetical protein	-
PSH69_RS25835 (PSH69_25825)	lanM	5751574..5754660 (+)	3087	WP_305410202.1	type 2 lanthipeptide synthetase LanM	-
PSH69_RS25840 (PSH69_25830)	yjiA	5754733..5755695 (-)	963	WP_305410203.1	GTPase	-
PSH69_RS25845 (PSH69_25835)	-	5755816..5756013 (-)	198	WP_007937254.1	YbdD/YjiX family protein	-
PSH69_RS25850 (PSH69_25840)	-	5756029..5758095 (-)	2067	WP_305410204.1	carbon starvation CstA family protein	-
PSH69_RS25855 (PSH69_25845)	-	5758256..5758624 (+)	369	WP_305410205.1	PilZ domain-containing protein	-
PSH69_RS25860 (PSH69_25850)	radA	5758677..5760044 (-)	1368	WP_019581287.1	DNA repair protein RadA	Machinery gene
PSH69_RS25865 (PSH69_25855)	-	5760090..5760644 (-)	555	WP_111454233.1	ankyrin repeat domain-containing protein	-
PSH69_RS25870 (PSH69_25860)	katB	5760702..5762243 (-)	1542	WP_305410206.1	catalase KatB	-
PSH69_RS25875 (PSH69_25865)	mscL	5762529..5762942 (+)	414	WP_305410207.1	large-conductance mechanosensitive channel protein MscL	-
PSH69_RS25880 (PSH69_25870)	-	5762987..5763763 (-)	777	WP_305410208.1	ferredoxin--NADP reductase	-
PSH69_RS25885 (PSH69_25875)	-	5764052..5764759 (+)	708	WP_305410209.1	autoinducer binding domain-containing protein	-
PSH69_RS25890 (PSH69_25880)	-	5764944..5766068 (+)	1125	WP_305410210.1	class I SAM-dependent methyltransferase	-
PSH69_RS25895 (PSH69_25885)	-	5766139..5766306 (-)	168	WP_081625770.1	DUF2474 domain-containing protein	-
PSH69_RS25900 (PSH69_25890)	cydB	5766364..5767371 (-)	1008	WP_018925814.1	cytochrome d ubiquinol oxidase subunit II	-
PSH69_RS25905 (PSH69_25895)	-	5767375..5768814 (-)	1440	WP_305410211.1	cytochrome ubiquinol oxidase subunit I	-
PSH69_RS25910 (PSH69_25900)	-	5769218..5770456 (+)	1239	WP_305410212.1	MFS transporter	-
PSH69_RS25915 (PSH69_25905)	-	5770466..5771017 (-)	552	WP_305410213.1	DJ-1 family glyoxalase III	-
PSH69_RS25920 (PSH69_25910)	-	5771085..5771540 (-)	456	WP_305412875.1	DUF4879 domain-containing protein	-
PSH69_RS25925 (PSH69_25915)	-	5771768..5773063 (+)	1296	WP_018925809.1	NCS2 family permease	-
PSH69_RS25930 (PSH69_25920)	trmA	5773060..5774139 (+)	1080	WP_305410214.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
PSH69_RS25935 (PSH69_25925)	-	5774192..5774638 (-)	447	WP_305410215.1	DUF2442 domain-containing protein	-
PSH69_RS25940 (PSH69_25930)	-	5774635..5775063 (-)	429	WP_305410216.1	DUF4160 domain-containing protein	-

Sequence

Protein

• Length: 455 a.a.
• Molecular weight: 48787.24 Da
• Isoelectric Point: 6.8987

>NTDB_id=785736 PSH69_RS25860 WP_019581287.1 5758677..5760044(-) (radA) [Pseudomonas sp. FP1740]
MAKAKRMYGCTECGSTFPKWAGQCGECGAWNTLTETMVESGGAAAPSGRTGWTGQQAQIKTLAEVSVEEIPRFSTASSEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEPPPGLQIIAVTRLEQALDALFE

Nucleotide

• Length: 1368 bp

>NTDB_id=785736 PSH69_RS25860 WP_019581287.1 5758677..5760044(-) (radA) [Pseudomonas sp. FP1740]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGTGGCTCAACCTTCCCCAAGTGGGCCGGCCAGTGCGGCGAATG
CGGGGCCTGGAACACACTGACCGAAACCATGGTGGAGAGTGGCGGCGCCGCAGCCCCCAGCGGTCGCACCGGTTGGACCG
GCCAGCAGGCGCAGATCAAGACCCTGGCCGAAGTCAGCGTCGAAGAAATTCCGCGGTTCTCCACGGCGTCCAGTGAGCTC
GACCGTGTCTTGGGCGGTGGTCTGGTGGATGGCTCGGTGGTGTTGATCGGTGGTGACCCCGGTATCGGCAAGTCGACCAT
TCTGTTGCAAACCTTGTGCAACCTCGCCAAAAGCATGCCGGCGCTGTATGTCACTGGCGAAGAGTCCCAGCAACAAGTGG
CCATGCGTGCCCGACGGCTGGGCTTGCCTCAGGATCAACTGCGGGTGATGACTGAAACCTGCATTGAAACCATCATTGCC
ACCGCTCGGCAGGAAAAGCCCAAGGTGATGGTGATCGACTCGATTCAGACGATCTTCACCGAGCAACTGCAATCGGCGCC
GGGGGGCGTTTCCCAGGTACGCGAAAGCGCGGCATTGCTGGTGCGTTACGCCAAGCAAAGCGGCACGGCGATTTTCCTCG
TGGGCCATGTCACTAAAGAAGGTGCGCTGGCCGGCCCCCGAGTGCTGGAGCACATGGTCGACACCGTTCTGTATTTCGAA
GGGGAATCCGATGGGCGCCTGCGTTTGCTGCGGGCGGTGAAAAACCGGTTCGGCGCGGTGAATGAACTGGGCGTGTTCGG
CATGACCGACAAAGGTCTGAAAGAAGTCTCCAACCCTTCGGCGATTTTCCTCACTCGCGCTCAGGAAGAAGTCCCGGGCA
GCGTGGTCATGGCCACATGGGAAGGCACCCGGCCGATGCTGGTGGAAGTCCAGGCGTTGGTGGACGACAGTCATCTGGCC
AACCCGCGCCGGGTGACGCTCGGTCTGGATCAGAATCGCCTGGCCATGTTGTTGGCGGTTTTGCACCGCCACGGCGGCAT
CCCGACCCACGATCAGGACGTGTTCCTCAACGTGGTCGGCGGCGTGAAGGTCCTGGAAACCGCTTCCGACCTGGCCTTGA
TGGCGGCGGTCATGTCCAGTTTGCGCAACCGGCCGCTGCCGCACGATCTGCTGGTGTTTGGCGAAGTCGGGTTGTCGGGT
GAAGTACGCCCGGTACCGAGTGGCCAGGAGCGCTTGAAAGAAGCTGCCAAGCACGGCTTCAAACGCGCCATCGTGCCCAA
GGGCAACGCGCCGAAAGAGCCGCCGCCGGGGTTGCAGATTATCGCCGTGACGCGCCTGGAACAGGCCCTCGACGCACTGT
TCGAATAA

Domains

Predicted by InterproScan.

• AAA domain (AAA_25) (74-221)

• Subunit ChlI of Mg-chelatase (ChlI) (353-429)

• Rubredoxin metal binding domain (Zn_ribbon_LapB) (8-35)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
radA	Bacillus subtilis subsp. subtilis str. 168	49.015	100	0.492
radA	Streptococcus pneumoniae Rx1	46.522	100	0.47
radA	Streptococcus pneumoniae R6	46.522	100	0.47
radA	Streptococcus pneumoniae TIGR4	46.522	100	0.47
radA	Streptococcus pneumoniae D39	46.522	100	0.47
radA	Streptococcus mitis SK321	46.711	100	0.468
radA	Streptococcus mitis NCTC 12261	46.491	100	0.466