Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSH73_RS01800 Genome accession   NZ_CP117441
Coordinates   413714..415045 (+) Length   443 a.a.
NCBI ID   WP_305462678.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2294     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408714..420045
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH73_RS01780 (PSH73_01780) pilM 411311..411964 (+) 654 WP_057958078.1 type IV pilus biogenesis protein PilM -
  PSH73_RS01785 (PSH73_01785) - 411964..412515 (+) 552 WP_169989265.1 PilN domain-containing protein -
  PSH73_RS01790 (PSH73_01790) - 412512..413132 (+) 621 WP_252117358.1 type 4a pilus biogenesis protein PilO -
  PSH73_RS01795 (PSH73_01795) - 413129..413644 (+) 516 WP_170044447.1 pilus assembly protein PilP -
  PSH73_RS01800 (PSH73_01800) pilQ 413714..415045 (+) 1332 WP_305462678.1 type IV pilus secretin PilQ Machinery gene
  PSH73_RS01805 (PSH73_01805) aroK 415050..415568 (+) 519 WP_057437234.1 shikimate kinase AroK -
  PSH73_RS01810 (PSH73_01810) aroB 415716..416816 (+) 1101 WP_057958082.1 3-dehydroquinate synthase -
  PSH73_RS01815 (PSH73_01815) - 416827..418401 (+) 1575 WP_057958083.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 443 a.a.        Molecular weight: 48363.24 Da        Isoelectric Point: 4.8378

>NTDB_id=785178 PSH73_RS01800 WP_305462678.1 413714..415045(+) (pilQ) [Pseudomonas sp. FP2294]
MTEAVPKRIDLIQLPPPGSSPLNTARGPFTGNRLSLNFQDIEVRAVLQQIADVAGLNLVAGDDVQGSITLRLKDVPWDQA
LDLVLQAKGLDRRVTANVLLVAPAEELAARELLALESRKLMTELTPLRRELLQVNYAKASELAKLFQSVTGLEGATDERG
SVTVDDRTNNIIAYQTGERLEELRRIVSQLDVPVRQVMIEARIVEANVDYDKSVGVRWSGLLRNKGNWSGGGVDKKPSGE
GESPDSSPFVDLGVVSPNSGLGIAFITDNVLLDLELTAMEKGGNGEVISQPKVVTSNKETARILKGTEIPYQEATSEGAT
SVSFKEASLSLEVTPQITPDDQVIMEVRVTKDEPDYLNKLNDVPPIKKNEVNAKVLVRDGETIVLGGVFSKNQSKVVDKV
PFLGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1332 bp        

>NTDB_id=785178 PSH73_RS01800 WP_305462678.1 413714..415045(+) (pilQ) [Pseudomonas sp. FP2294]
ATGACTGAGGCTGTGCCCAAGCGAATCGACCTGATTCAATTGCCTCCTCCCGGTAGTTCGCCCTTGAACACTGCGCGTGG
CCCATTTACCGGCAACAGGCTGTCCCTCAACTTTCAGGACATTGAGGTGCGCGCCGTCTTGCAGCAGATTGCCGATGTGG
CCGGGCTCAACCTGGTGGCCGGTGATGATGTGCAAGGCTCGATAACCCTGCGCCTCAAGGATGTGCCGTGGGACCAGGCC
CTGGACCTGGTGTTGCAGGCCAAGGGCCTGGACCGGCGGGTGACCGCCAACGTGCTGCTGGTGGCCCCGGCCGAGGAACT
GGCCGCACGGGAGTTGCTGGCCCTGGAGTCGCGCAAGTTGATGACCGAGCTGACGCCACTGCGTCGGGAGCTGCTGCAAG
TCAATTACGCCAAGGCGTCTGAGCTGGCCAAGCTGTTCCAGTCGGTGACCGGGCTTGAGGGGGCGACCGATGAGCGCGGT
TCGGTGACGGTGGATGACCGCACCAACAACATCATCGCCTACCAGACGGGCGAGCGCCTGGAGGAGCTGCGGCGCATCGT
CAGTCAGTTGGATGTGCCGGTACGCCAGGTGATGATCGAGGCGCGGATAGTCGAGGCCAATGTCGACTATGACAAAAGCG
TGGGGGTGCGCTGGAGCGGGTTGCTGCGCAATAAAGGTAATTGGAGTGGGGGTGGGGTCGATAAAAAACCGTCCGGTGAG
GGCGAGTCGCCCGATAGCTCACCGTTCGTTGACCTGGGCGTGGTCAGCCCGAACTCCGGCCTGGGCATTGCCTTTATCAC
CGATAACGTGCTGCTGGACCTGGAACTCACGGCCATGGAAAAAGGCGGCAATGGCGAAGTGATTTCCCAACCCAAGGTGG
TCACCTCCAACAAGGAAACCGCGCGGATTCTCAAGGGCACCGAGATTCCTTACCAGGAAGCCACGTCTGAGGGGGCGACC
TCGGTCTCGTTCAAGGAAGCCTCGCTGTCCCTGGAGGTGACGCCGCAAATCACCCCCGATGACCAGGTGATCATGGAGGT
TCGGGTGACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAGAACGAGGTCAACGCCA
AGGTTCTGGTCAGGGACGGCGAGACCATCGTCCTCGGTGGGGTTTTCTCCAAAAACCAAAGCAAGGTGGTAGATAAAGTG
CCATTTTTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGTTGCCCAAAGAAAATCCGAGCTGCTGGT
ATTCCTGACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(278-434)

Secretin and TonB N terminus short domain (STN)

(57-103)

Bacterial type II/III secretion system short domain (Secretin_N)

(131-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.794

98.646

0.688
  pilQ Acinetobacter baumannii strain A118

41.81

100

0.438
  pilQ Acinetobacter baumannii D1279779

41.81

100

0.438
  comQ Acinetobacter baylyi ADP1

42.35

100

0.431
  pilQ Legionella pneumophila strain ERS1305867

44.762

94.808

0.424
  pilQ Vibrio campbellii strain DS40M4

44.208

95.485

0.422
  pilQ Vibrio cholerae strain A1552

44.63

94.582

0.422
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

44.63

94.582

0.422
  pilQ Legionella pneumophila strain Lp02

44.286

94.808

0.42
  pilQ Neisseria gonorrhoeae strain FA1090

37.097

97.968

0.363