Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   LLL8_RS03035 Genome accession   NZ_AP025700
Coordinates   588210..590438 (+) Length   742 a.a.
NCBI ID   WP_251900221.1    Uniprot ID   -
Organism   Lactococcus lactis strain L8     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 583210..595438
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS03000 (LLL8_05670) - 583699..584367 (+) 669 WP_003129542.1 hypothetical protein -
  LLL8_RS03005 (LLL8_05680) - 584537..585433 (+) 897 WP_003129543.1 SPFH domain-containing protein -
  LLL8_RS03015 (LLL8_05690) - 585809..586069 (+) 261 WP_003129544.1 SemiSWEET family transporter -
  LLL8_RS03025 (LLL8_05700) rnz 586426..587349 (+) 924 WP_003129545.1 ribonuclease Z -
  LLL8_RS03030 (LLL8_05710) - 587342..588043 (+) 702 WP_251900219.1 SDR family oxidoreductase -
  LLL8_RS03035 (LLL8_05720) recJ 588210..590438 (+) 2229 WP_251900221.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  LLL8_RS03040 (LLL8_05730) - 590561..591073 (+) 513 WP_012897343.1 adenine phosphoribosyltransferase -
  LLL8_RS03045 (LLL8_05740) rpoE 591290..591853 (+) 564 WP_012897344.1 DNA-directed RNA polymerase subunit delta -
  LLL8_RS03050 (LLL8_05750) mltG 591990..593642 (+) 1653 WP_251900222.1 endolytic transglycosylase MltG -
  LLL8_RS03055 (LLL8_05760) greA 593702..594175 (+) 474 WP_025016857.1 transcription elongation factor GreA -
  LLL8_RS03060 (LLL8_05770) - 594353..594808 (+) 456 WP_010905471.1 CtsR family transcriptional regulator -

Sequence

Protein


Download         Length: 742 a.a.        Molecular weight: 83486.56 Da        Isoelectric Point: 4.7534

>NTDB_id=78517 LLL8_RS03035 WP_251900221.1 588210..590438(+) (recJ) [Lactococcus lactis strain L8]
MIKAKYDWKVADTAISEDFLKIAKKHKLDELTSRVLYQRGIHAEAEIEQFLKPSLENLHDPFLLHDMEKATGRILSAIEQ
GENILIYGDYDADGMTASSVMKSALDELGAEAQVYLPNRFTDGYGPNLDVYQYYIKNENINLIITVDNGVAGLEAITWAQ
ENGVDVIVTDHHSIPDQLPPAYAIVHPEHPDSQYPFKYLAGVGVAFKVACALLEYAPSEMLDLVAIGTIADMVSLTDENR
ILVAHGLKVLAQTERAGLQELMKYAGVDFDKITEETVGFQIAPRLNALGRLDDPNPAIELLTGWDEDEAHEIAKMIDQKN
SERKEIVEKIHNEALSMLTDEPVQILYHKDWHKGVLGIVAGRLLEAIHKPVIMLAQEDGILRGSARSIENFDIFKALNAH
RELFIAFGGHKQAAGMTLSLENVEAVKKAMIDYIVDNHLDMSKKSALEVADRCHLDDISLSTITNLAKLAPFGMDNPKPR
FLIEDYKVIQSRSMGKDNAHLKLKIQEEKQQIDAVYFQHGSEELEFEQAQTKLVATLSSNSWNGNTSLQLMIEDADSVGV
ELLDIRSKQIPIPKEANIFSQNQLKHGIMEDVLVIEETPGDLAGLSVLKEAVSKAASDDFKMIYFKNKITDSYYLTGSGT
REEFARLYKAIYQFPEFDIRYKLKSLADYLKIPHLLLVKMIKIFEELEFVSIDNGLMTVNKTADKREISESTIYQELEKI
VKMQELFALAPVKEIYQNLLEK

Nucleotide


Download         Length: 2229 bp        

>NTDB_id=78517 LLL8_RS03035 WP_251900221.1 588210..590438(+) (recJ) [Lactococcus lactis strain L8]
ATGATAAAAGCAAAATATGATTGGAAAGTGGCTGATACAGCCATTTCCGAAGATTTTCTAAAGATAGCGAAAAAACATAA
ATTAGATGAATTGACAAGTCGCGTGCTTTATCAAAGAGGAATTCATGCAGAAGCTGAAATTGAGCAATTTTTAAAGCCAA
GTTTAGAAAATTTACATGACCCTTTTCTACTTCATGATATGGAAAAAGCAACAGGGCGTATTTTATCAGCAATTGAGCAA
GGTGAAAACATCTTAATTTATGGTGACTATGATGCTGACGGAATGACTGCATCATCAGTCATGAAATCCGCTCTGGATGA
ATTAGGAGCTGAGGCTCAAGTTTATTTACCCAATCGTTTTACTGACGGTTACGGGCCGAATCTTGACGTTTATCAGTACT
ATATTAAGAATGAAAATATCAATCTTATTATTACTGTAGATAATGGGGTTGCTGGCTTGGAGGCAATTACCTGGGCACAG
GAAAATGGAGTGGATGTAATTGTTACCGACCATCACTCAATTCCTGACCAACTTCCCCCTGCTTATGCCATTGTCCACCC
CGAACATCCTGACAGTCAATATCCTTTCAAATATTTGGCAGGAGTTGGTGTCGCCTTCAAAGTTGCCTGTGCTCTTTTAG
AATATGCACCCAGTGAAATGCTTGATTTAGTTGCAATTGGTACAATTGCTGACATGGTCAGTTTGACTGATGAAAACCGA
ATTTTAGTGGCTCATGGTCTAAAAGTTTTGGCCCAAACAGAGCGTGCCGGCCTACAAGAATTAATGAAGTATGCTGGGGT
TGATTTTGATAAAATCACTGAGGAAACGGTTGGTTTTCAAATTGCTCCCCGTTTAAACGCTTTGGGACGTCTCGATGACC
CAAACCCAGCCATTGAATTGCTGACAGGTTGGGATGAAGATGAAGCGCATGAAATTGCCAAAATGATTGACCAAAAAAAT
TCAGAGCGAAAAGAAATTGTGGAAAAAATTCACAATGAAGCTCTGAGTATGCTGACAGATGAACCTGTTCAGATTTTATA
TCATAAGGACTGGCATAAAGGTGTCTTAGGAATTGTTGCGGGTCGCTTGCTAGAAGCCATACATAAGCCAGTAATCATGC
TGGCCCAAGAAGATGGGATTTTGCGTGGGTCTGCTCGGTCAATCGAAAATTTTGATATTTTCAAGGCATTAAATGCCCAT
CGTGAACTTTTTATTGCTTTTGGTGGTCATAAACAAGCCGCAGGGATGACTTTAAGTCTGGAAAACGTAGAAGCAGTTAA
AAAGGCGATGATTGATTATATCGTGGACAATCATTTAGATATGTCTAAAAAAAGTGCCTTAGAAGTTGCTGACCGTTGCC
ACTTAGACGATATTTCGCTCTCAACAATCACTAATTTAGCAAAATTGGCTCCCTTTGGCATGGATAATCCTAAGCCTCGA
TTTTTAATCGAAGATTATAAGGTGATTCAAAGCCGAAGTATGGGGAAAGACAATGCTCATCTTAAATTAAAAATTCAAGA
AGAAAAGCAACAAATTGATGCCGTTTATTTCCAACATGGTTCAGAAGAATTGGAATTCGAGCAGGCACAAACAAAATTAG
TCGCAACACTTTCAAGTAATAGTTGGAATGGAAATACGAGTCTTCAATTAATGATTGAAGATGCTGATTCAGTTGGTGTC
GAACTTCTTGATATAAGAAGTAAACAAATTCCTATCCCAAAAGAGGCTAATATTTTTTCTCAAAATCAGCTAAAACATGG
TATAATGGAAGATGTGCTTGTCATCGAAGAGACACCTGGAGATTTAGCAGGTTTGTCAGTATTAAAAGAAGCTGTCAGTA
AAGCAGCGTCGGATGATTTTAAAATGATTTATTTTAAAAATAAAATCACTGATAGCTATTATTTAACAGGAAGTGGAACA
CGAGAAGAATTTGCAAGACTTTATAAAGCAATTTATCAATTTCCAGAATTTGATATTCGCTATAAATTAAAATCACTTGC
GGATTATTTGAAAATCCCTCATCTCTTGCTTGTCAAGATGATAAAAATCTTTGAGGAACTTGAATTTGTCAGCATTGACA
ATGGCTTAATGACCGTTAATAAGACGGCCGATAAACGCGAAATTTCTGAGAGTACAATTTATCAAGAACTAGAAAAAATT
GTAAAAATGCAAGAACTTTTTGCTTTAGCACCCGTCAAAGAAATTTATCAAAATTTATTAGAAAAATAA

Domains

Predicted by InterProScan.

DHH family (DHH)

(83-228)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(592-737)

RecJ OB domain (RecJ_OB)

(454-554)

DHHA1 domain (DHHA1)

(317-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Streptococcus pneumoniae strain D39V

56.913

100

0.571
  recJ Bacillus subtilis subsp. subtilis str. 168

36.457

100

0.383

Multiple sequence alignment