Detailed information NTG0784946

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH65_RS13710
• Genome accession: NZ_CP117431
• Coordinates: 2933688..2934797 (+)
• Length: 369 a.a.
• NCBI ID: WP_305426658.1
• Uniprot ID: -
• Organism: Pseudomonas sp. FP603
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2928688..2939797

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH65_RS13695 (PSH65_13695)	-	2929568..2930500 (+)	933	WP_305426657.1	sugar kinase	-
PSH65_RS13700 (PSH65_13700)	-	2930689..2930964 (-)	276	WP_007898282.1	peptidylprolyl isomerase	-
PSH65_RS13705 (PSH65_13705)	-	2931031..2933568 (-)	2538	Protein_2703	PAS domain S-box protein	-
PSH65_RS13710 (PSH65_13710)	pilU	2933688..2934797 (+)	1110	WP_305426658.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH65_RS13715 (PSH65_13715)	-	2934909..2936864 (-)	1956	WP_305426659.1	acetoacetate--CoA ligase	-
PSH65_RS13720 (PSH65_13720)	hbdH	2937009..2937782 (-)	774	WP_305426660.1	3-hydroxybutyrate dehydrogenase	-
PSH65_RS13725 (PSH65_13725)	-	2937790..2939181 (-)	1392	WP_305426661.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40875.80 Da
• Isoelectric Point: 6.7471

>NTDB_id=784946 PSH65_RS13710 WP_305426658.1 2933688..2934797(+) (pilU) [Pseudomonas sp. FP603]
MEIDALLQILASRNGSDLYLSTGAPPSAKFDGVLKALAEHPFKPGEVAAIAASIMDAEQRLEFDRELEMNLALSLAGVGR
FRLNIFKQRNDVSIVARNIKLDIPRFEDLKLPPVLLETIMLKQGLILFVGSTGSGKSTSLAALIDYRNRHSSGHIITIED
PVEFIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRTQLLHDLGNNLKAFVSQRLVRTRDGQRRAAVEVMLGTPTIGDLIRRNEFSELKGIMEKSSEAGMQTFDGAL
FALFAEGAIDEAEALKHADSVNNLRLRLKMHAETTPGPHTPPGEWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=784946 PSH65_RS13710 WP_305426658.1 2933688..2934797(+) (pilU) [Pseudomonas sp. FP603]
ATGGAAATCGATGCACTGTTGCAGATTCTGGCCAGCCGCAATGGCTCCGATCTTTACCTGTCCACGGGCGCGCCACCCAG
TGCGAAATTTGACGGTGTGCTCAAAGCCCTGGCCGAGCACCCGTTCAAGCCGGGTGAAGTCGCGGCCATCGCGGCGTCCA
TTATGGACGCCGAACAGCGCCTGGAGTTCGATCGCGAACTGGAAATGAACCTGGCGCTTTCCCTGGCCGGGGTCGGGCGT
TTTCGGCTCAATATCTTCAAGCAGCGCAACGATGTGTCTATCGTGGCGCGCAACATCAAACTCGACATTCCACGCTTCGA
AGACCTGAAGTTGCCACCGGTGCTGCTCGAAACCATCATGCTCAAACAGGGGCTGATACTGTTCGTCGGTTCGACCGGTT
CGGGCAAGTCGACCTCGCTGGCAGCACTGATCGATTACCGCAATCGCCACAGCAGCGGCCATATCATCACCATCGAAGAC
CCGGTCGAGTTTATCCATCGGCACAAGAAGTCGATCATCAACCAGCGCGAGGTCGGCGTTGATACCCGCAGTTTTCATGC
AGCATTGAAAAACACCCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATTCGCGATCGCGAGACTATGGAGCACG
CGCTGGCGTTTGCCGATACCGGTCATCTGGTAATTTCCACGTTGCATGCGCACAACGCCAATCAGGCGCTGGACCGCGTG
ATCAATTTCTTCCCCGAAGAACGTCGCACGCAACTGCTCCATGACCTGGGCAATAACCTCAAGGCCTTTGTTTCCCAGCG
GTTGGTGCGGACCCGCGATGGGCAGCGCCGGGCGGCTGTGGAGGTGATGCTGGGAACGCCGACCATCGGCGACCTGATCC
GGCGCAATGAGTTTTCCGAGCTCAAGGGCATTATGGAAAAGTCTTCAGAGGCGGGGATGCAGACGTTTGATGGGGCGCTG
TTCGCGCTGTTCGCCGAGGGCGCGATTGATGAGGCAGAGGCGTTGAAGCATGCGGATTCGGTGAACAACTTGCGCTTGCG
CCTGAAAATGCACGCCGAGACTACGCCCGGGCCCCATACACCGCCGGGGGAATGGGGGTTAGTGGACTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.472	100	0.545
pilU	Acinetobacter baylyi ADP1	52.958	96.206	0.509
pilU	Vibrio cholerae strain A1552	50.857	94.851	0.482
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.742	90.244	0.377
pilT	Vibrio cholerae strain A1552	41.742	90.244	0.377
pilT	Pseudomonas stutzeri DSM 10701	40.407	93.225	0.377
pilT	Pseudomonas aeruginosa PAK	40.116	93.225	0.374
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.823	91.87	0.366
pilT	Legionella pneumophila strain ERS1305867	40.419	90.515	0.366
pilT	Legionella pneumophila strain Lp02	40.419	90.515	0.366