Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSH74_RS24490 Genome accession   NZ_CP117426
Coordinates   5437905..5439122 (+) Length   405 a.a.
NCBI ID   WP_305385933.1    Uniprot ID   -
Organism   Pseudomonas hefeiensis strain FP821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5432905..5444122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH74_RS24470 (PSH74_24470) - 5434302..5434598 (+) 297 WP_305385929.1 DUF2845 domain-containing protein -
  PSH74_RS24475 (PSH74_24475) - 5434924..5435277 (-) 354 WP_305385930.1 BON domain-containing protein -
  PSH74_RS24480 (PSH74_24480) pilA 5435557..5435976 (-) 420 WP_305385931.1 pilin Machinery gene
  PSH74_RS24485 (PSH74_24485) pilB 5436202..5437902 (+) 1701 WP_305385932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH74_RS24490 (PSH74_24490) pilC 5437905..5439122 (+) 1218 WP_305385933.1 type II secretion system F family protein Machinery gene
  PSH74_RS24495 (PSH74_24495) pilD 5439124..5439993 (+) 870 WP_305416169.1 A24 family peptidase Machinery gene
  PSH74_RS24500 (PSH74_24500) coaE 5440005..5440628 (+) 624 WP_305385934.1 dephospho-CoA kinase -
  PSH74_RS24505 (PSH74_24505) yacG 5440625..5440825 (+) 201 WP_305385935.1 DNA gyrase inhibitor YacG -
  PSH74_RS24510 (PSH74_24510) - 5440926..5441141 (-) 216 WP_305385936.1 hypothetical protein -
  PSH74_RS24515 (PSH74_24515) - 5441205..5441891 (-) 687 WP_305385937.1 energy-coupling factor ABC transporter permease -
  PSH74_RS24520 (PSH74_24520) - 5442036..5442662 (+) 627 WP_305385938.1 DUF1780 domain-containing protein -
  PSH74_RS24525 (PSH74_24525) - 5442659..5443186 (+) 528 WP_305385939.1 MOSC domain-containing protein -
  PSH74_RS24530 (PSH74_24530) - 5443239..5443412 (+) 174 WP_214918225.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44140.88 Da        Isoelectric Point: 10.0551

>NTDB_id=784816 PSH74_RS24490 WP_305385933.1 5437905..5439122(+) (pilC) [Pseudomonas hefeiensis strain FP821]
MAVKALKTDIYTWEGKDRKGTKMTGELTGQSPALVKAQLRKQGINPGKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQQWWWILLGGAVAAFFGVK
HALKRSQGLRDWRDKWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIRQDVSTGM
QLNFSMRASGIFPNMAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=784816 PSH74_RS24490 WP_305385933.1 5437905..5439122(+) (pilC) [Pseudomonas hefeiensis strain FP821]
ATGGCGGTCAAGGCACTAAAAACCGACATTTATACGTGGGAAGGCAAAGACCGCAAAGGCACGAAAATGACCGGCGAACT
GACCGGCCAGAGCCCGGCCCTGGTCAAGGCTCAGTTGCGCAAACAGGGCATCAACCCAGGCAAGGTACGCAAGAAATCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCATTGGACATCGCCCTGTTCACTCGTCAGATGGCAACCATGCTCAAG
GCTGGCGTGCCCTTGTTGCAGGCGTTCGACATCATCGGCGAAGGCTTCGACAACGCCAATATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCGTCGTTGCGCAAATGCCCGCAATACTTCGACGAGCTGTATT
GCAATCTGGTGGACGCCGGTGAACAGGCTGGCGCCCTGGATACGCTGCTGGAGCGCGTCGCGACCTACAAGGAAAAAAGC
GAAGCGCTCAAGGCCAAGATCAAGAAGGCCATGACCTACCCGGCCGCCGTAGTGCTCGTCGCCGCCGTGGTCACGGGTAT
TCTGCTGGTCAAGGTGGTGCCACAGTTCGAATCGGTGTTCTCCGGGTTCGGCGCCCAACTGCCGGCGTTCACGGTGATGG
TCATCGGCCTGTCGGAATTCATGCAGCAGTGGTGGTGGATACTGCTAGGCGGCGCGGTGGCTGCGTTCTTCGGTGTCAAA
CACGCACTCAAGCGCTCCCAGGGCTTGCGTGACTGGCGCGATAAATGGCTGCTTAAACTGCCGCTGATCGGCACCCTGAT
GTACAAATCCGCCGTGGCCCGCTATGCCCGGACCCTTTCCACGACTTTCGCAGCCGGCGTACCGCTGGTGGAAGCCCTGG
ACTCGGTATCAGGCGCCACCGGCAACGTGGTGTTCAAACGCGCGGTACAACGCATCCGGCAGGATGTCTCGACCGGTATG
CAGTTGAATTTTTCCATGCGTGCTTCAGGCATATTCCCGAACATGGCAATCCAGATGACCGCCATTGGCGAGGAGTCCGG
CGCGCTGGACGACATGCTCGACAAAGTGGCGAGCTTTTATGAAGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTGGTCGTCGGCGGCCTGGTGGTTGCCATGTACCTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

73.58

100

0.736
  pilC Acinetobacter baylyi ADP1

59.601

99.012

0.59
  pilC Acinetobacter baumannii D1279779

58.333

100

0.588
  pilC Legionella pneumophila strain ERS1305867

55.5

98.765

0.548
  pilC Vibrio cholerae strain A1552

43.704

100

0.437
  pilC Vibrio campbellii strain DS40M4

43.687

97.778

0.427
  pilG Neisseria meningitidis 44/76-A

42.716

100

0.427
  pilG Neisseria gonorrhoeae MS11

42.537

99.259

0.422
  pilC Thermus thermophilus HB27

38.653

99.012

0.383