Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PSH74_RS24480 Genome accession   NZ_CP117426
Coordinates   5435557..5435976 (-) Length   139 a.a.
NCBI ID   WP_305385931.1    Uniprot ID   -
Organism   Pseudomonas hefeiensis strain FP821     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5430557..5440976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH74_RS24465 (PSH74_24465) - 5431367..5434300 (+) 2934 WP_305416167.1 DUF748 domain-containing protein -
  PSH74_RS24470 (PSH74_24470) - 5434302..5434598 (+) 297 WP_305385929.1 DUF2845 domain-containing protein -
  PSH74_RS24475 (PSH74_24475) - 5434924..5435277 (-) 354 WP_305385930.1 BON domain-containing protein -
  PSH74_RS24480 (PSH74_24480) pilA 5435557..5435976 (-) 420 WP_305385931.1 pilin Machinery gene
  PSH74_RS24485 (PSH74_24485) pilB 5436202..5437902 (+) 1701 WP_305385932.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSH74_RS24490 (PSH74_24490) pilC 5437905..5439122 (+) 1218 WP_305385933.1 type II secretion system F family protein Machinery gene
  PSH74_RS24495 (PSH74_24495) pilD 5439124..5439993 (+) 870 WP_305416169.1 A24 family peptidase Machinery gene
  PSH74_RS24500 (PSH74_24500) coaE 5440005..5440628 (+) 624 WP_305385934.1 dephospho-CoA kinase -
  PSH74_RS24505 (PSH74_24505) yacG 5440625..5440825 (+) 201 WP_305385935.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 13938.05 Da        Isoelectric Point: 7.5897

>NTDB_id=784814 PSH74_RS24480 WP_305385931.1 5435557..5435976(-) (pilA) [Pseudomonas hefeiensis strain FP821]
MNKQNGFTLIELLIVVAIIGILATFALPQYSKYQARAKATAGLAEISALKVPYEDAMNQGTDPSVANMSITSPTSNCTIT
ITGTAAAGAGTIVCTLANAPAAVAGKTITLSRSATGAWACTSTAVQEFLPAGCTGAAAP

Nucleotide


Download         Length: 420 bp        

>NTDB_id=784814 PSH74_RS24480 WP_305385931.1 5435557..5435976(-) (pilA) [Pseudomonas hefeiensis strain FP821]
ATGAATAAGCAAAACGGTTTTACCTTGATTGAGTTGCTGATCGTTGTGGCGATCATCGGCATCCTGGCGACGTTTGCGTT
GCCGCAGTATTCCAAGTATCAAGCGCGGGCGAAGGCTACGGCGGGGCTGGCGGAAATTTCGGCGCTGAAGGTGCCCTATG
AAGACGCTATGAATCAAGGTACCGATCCATCGGTGGCTAATATGAGCATTACGTCTCCTACCAGTAATTGCACCATTACG
ATCACCGGTACAGCAGCAGCGGGAGCAGGCACCATCGTTTGTACGCTCGCGAACGCCCCTGCTGCCGTAGCAGGTAAGAC
GATCACTCTTTCTCGTTCAGCTACCGGCGCTTGGGCGTGCACCTCGACCGCAGTCCAGGAATTCTTGCCCGCCGGTTGCA
CGGGTGCCGCTGCACCCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

48.551

99.281

0.482
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.985

98.561

0.453
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

97.122

0.424
  pilA Pseudomonas aeruginosa PAK

39.073

100

0.424
  comP Acinetobacter baylyi ADP1

39.073

100

0.424
  pilA Ralstonia pseudosolanacearum GMI1000

42.029

99.281

0.417
  pilA2 Legionella pneumophila strain ERS1305867

40

100

0.417
  pilA2 Legionella pneumophila str. Paris

38.514

100

0.41
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.241

100

0.388
  pilA Vibrio cholerae C6706

37.241

100

0.388
  pilA Vibrio cholerae strain A1552

37.241

100

0.388