Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PRK74_RS25125 Genome accession   NZ_CP117421
Coordinates   4876818..4878167 (-) Length   449 a.a.
NCBI ID   WP_047387211.1    Uniprot ID   -
Organism   Bacillus cereus strain 2-6A     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4871818..4883167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRK74_RS25090 (PRK74_25090) - 4871862..4872557 (-) 696 WP_000466510.1 hypothetical protein -
  PRK74_RS25095 (PRK74_25095) - 4872544..4873332 (-) 789 WP_000120044.1 hypothetical protein -
  PRK74_RS25100 (PRK74_25100) - 4873360..4873929 (-) 570 WP_001211058.1 hypothetical protein -
  PRK74_RS25105 (PRK74_25105) - 4874047..4874520 (-) 474 WP_000511548.1 hypothetical protein -
  PRK74_RS25110 (PRK74_25110) hpf 4874925..4875467 (-) 543 WP_000671186.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  PRK74_RS25115 (PRK74_25115) cspC 4875790..4875987 (-) 198 WP_001990088.1 cold shock protein CspC -
  PRK74_RS25120 (PRK74_25120) - 4876114..4876818 (-) 705 WP_033685746.1 ComF family protein -
  PRK74_RS25125 (PRK74_25125) comFA 4876818..4878167 (-) 1350 WP_047387211.1 ATP-dependent helicase ComFA Machinery gene
  PRK74_RS25130 (PRK74_25130) - 4878534..4879604 (-) 1071 WP_061139627.1 endo-1,4-beta-xylanase -
  PRK74_RS25135 (PRK74_25135) - 4879751..4879960 (-) 210 WP_273633967.1 hypothetical protein -
  PRK74_RS29480 - 4880000..4880065 (-) 66 Protein_4889 hypothetical protein -
  PRK74_RS25140 (PRK74_25140) - 4881009..4882436 (-) 1428 WP_128267199.1 NlpC/P60 family protein -
  PRK74_RS25145 (PRK74_25145) - 4882585..4882899 (-) 315 WP_000400857.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51036.92 Da        Isoelectric Point: 9.9516

>NTDB_id=784745 PRK74_RS25125 WP_047387211.1 4876818..4878167(-) (comFA) [Bacillus cereus strain 2-6A]
MLAGKQLLLEELSSDLQRELNDLKKKGEIVCVQGVKKKNSKYMCQRCGNVDRRLFASFLCKRCSKVCAYCRKCITMGRVS
ECAVLVRGIAERKREKNSNLLQWNGTLSTGQNLAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGINEALQKGERVCIAT
PRTDVVLELAPRLQEVFPYIKVAALYGGSVDKEKDAVLVVATTHQLLRYYRAFHVMVVDEIDAFPYCADQMLQYAVKQAM
KEKAARIYLTATPDETWKRKLKQGKQKGVIVSGRYHRHPLPVPLFCWCGNWKKNLIHKRIPRVLLQWLQTYLNKKFPIFL
FVPHVRYIEEISLLLKPLNNRIEGVYAEDPGRKEKVAAFRKGEIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEAPYGEVIYFHYGKTEAMVRAKKHIQGMNKNAKEQGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=784745 PRK74_RS25125 WP_047387211.1 4876818..4878167(-) (comFA) [Bacillus cereus strain 2-6A]
ATGCTAGCGGGGAAACAGTTGCTATTAGAAGAACTTTCTTCAGATTTACAGAGGGAATTAAACGATTTGAAAAAGAAGGG
AGAGATCGTATGTGTACAAGGTGTAAAAAAGAAGAATTCTAAATATATGTGCCAACGCTGCGGAAATGTAGATCGGCGGC
TATTTGCGTCGTTTTTATGTAAAAGGTGCAGTAAAGTGTGCGCATATTGCCGGAAGTGTATAACGATGGGGAGAGTAAGT
GAATGTGCTGTACTTGTTCGCGGGATTGCTGAAAGAAAGAGAGAAAAGAATTCAAATTTGTTACAGTGGAACGGGACGTT
GTCTACTGGCCAGAATTTGGCGGCGCAAGGGGTTATAGAGGCTATTAAGCAAAAAGAATCATTTTTTATTTGGGCTGTAT
GCGGGGCTGGGAAAACAGAGATGTTGTTTTACGGAATTAACGAAGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACG
CCGAGAACGGATGTTGTTCTGGAATTAGCACCGAGATTGCAAGAAGTATTTCCATATATAAAGGTAGCGGCTTTATATGG
AGGGAGTGTGGATAAAGAAAAAGATGCAGTACTAGTCGTTGCGACTACGCATCAATTATTACGTTATTATAGGGCGTTTC
ATGTCATGGTTGTAGATGAGATAGATGCTTTTCCATATTGTGCAGATCAAATGTTACAGTACGCGGTAAAACAAGCGATG
AAAGAAAAAGCGGCGCGTATTTATTTAACTGCGACTCCAGATGAAACGTGGAAGCGAAAACTTAAACAAGGTAAACAAAA
AGGTGTTATTGTTTCTGGACGATATCATCGTCATCCTTTGCCAGTTCCTTTATTTTGTTGGTGCGGGAATTGGAAAAAAA
ACCTCATTCATAAAAGAATTCCTCGAGTTTTACTACAGTGGTTACAAACATACTTAAATAAAAAATTCCCTATTTTTTTA
TTCGTCCCCCATGTGCGATATATAGAAGAAATAAGCTTGTTGTTAAAACCATTAAACAATCGAATTGAAGGTGTATATGC
AGAAGATCCAGGGAGAAAAGAAAAAGTAGCGGCTTTCAGAAAGGGAGAAATCCCATTATTAGTTACAACGACAATTTTAG
AGCGAGGCGTAACGGTGAAAAATTTGCAAGTTGCAGTTTTAGGGGCGGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATCGCGGGCCGAGCAGGGCGGAGCTTTGAAGCACCGTATGGAGAAGTCATTTATTTTCACTATGGCAAGACAGAGGC
GATGGTGCGCGCGAAAAAACATATTCAAGGTATGAATAAAAATGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(313-408)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.131

98.441

0.503
  comFA Latilactobacillus sakei subsp. sakei 23K

41.191

89.755

0.37