Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PRK74_RS20565 Genome accession   NZ_CP117421
Coordinates   4021747..4022790 (-) Length   347 a.a.
NCBI ID   WP_001013180.1    Uniprot ID   A0A9X5WLU9
Organism   Bacillus cereus strain 2-6A     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4016747..4027790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRK74_RS20520 (PRK74_20520) - 4017178..4017378 (-) 201 WP_000106083.1 YqzE family protein -
  PRK74_RS20525 (PRK74_20525) - 4017417..4017914 (-) 498 WP_000836650.1 shikimate kinase -
  PRK74_RS20530 (PRK74_20530) - 4018030..4018680 (-) 651 WP_000183860.1 2OG-Fe(II) oxygenase -
  PRK74_RS20535 (PRK74_20535) comGG 4018857..4019228 (-) 372 WP_273633785.1 competence type IV pilus minor pilin ComGG -
  PRK74_RS20540 (PRK74_20540) comGF 4019225..4019695 (-) 471 WP_000923098.1 competence type IV pilus minor pilin ComGF -
  PRK74_RS20545 (PRK74_20545) - 4019665..4019967 (-) 303 WP_000829471.1 hypothetical protein -
  PRK74_RS20550 (PRK74_20550) comGD 4019960..4020415 (-) 456 WP_000813142.1 competence type IV pilus minor pilin ComGD -
  PRK74_RS20555 (PRK74_20555) comGC 4020412..4020711 (-) 300 WP_001178686.1 competence type IV pilus major pilin ComGC -
  PRK74_RS20560 (PRK74_20560) comGB 4020723..4021754 (-) 1032 WP_128267104.1 competence type IV pilus assembly protein ComGB -
  PRK74_RS20565 (PRK74_20565) comGA 4021747..4022790 (-) 1044 WP_001013180.1 competence type IV pilus ATPase ComGA Machinery gene
  PRK74_RS20570 (PRK74_20570) - 4022996..4023691 (+) 696 WP_000434125.1 helix-turn-helix transcriptional regulator -
  PRK74_RS20575 (PRK74_20575) - 4023817..4024059 (+) 243 WP_000440721.1 DUF2626 domain-containing protein -
  PRK74_RS20580 (PRK74_20580) - 4024166..4025560 (+) 1395 WP_070184374.1 L-cystine transporter -
  PRK74_RS20585 (PRK74_20585) - 4025640..4026031 (-) 392 Protein_4007 hypothetical protein -
  PRK74_RS20590 (PRK74_20590) - 4026119..4026334 (-) 216 WP_001008326.1 DUF3912 family protein -
  PRK74_RS20595 (PRK74_20595) - 4026587..4026898 (+) 312 WP_001093237.1 hypothetical protein -
  PRK74_RS20600 (PRK74_20600) - 4026933..4027421 (-) 489 WP_128267105.1 hypothetical protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39157.52 Da        Isoelectric Point: 8.5482

>NTDB_id=784734 PRK74_RS20565 WP_001013180.1 4021747..4022790(-) (comGA) [Bacillus cereus strain 2-6A]
MNGIEIFANTILKEACRVQASDLHIVPRQKDVAVQLRIGKDLMTRQCIEKEFGEKLVSHFKFLASMDIGEKRKPQNGSLY
LQIDGQEVYLRLSTLPTVYQESLVIRLHLQASVQPLSHLSLFPSTAAKLLSFLRYSQGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIITLEDPVEKRSDDVLQIQINEKAGLTYETGLKAILRHDPDIILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDAKGAIIRFMDYGITRQEIEQSLLAVAAQRLVELKCPFCRGKCSTLCKSMRQVRQASIYELLYGHELKQALKEADGEC
VTYKHETLESSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=784734 PRK74_RS20565 WP_001013180.1 4021747..4022790(-) (comGA) [Bacillus cereus strain 2-6A]
ATGAATGGGATTGAAATTTTTGCGAATACAATTTTAAAAGAAGCGTGTAGGGTACAAGCGTCAGATTTACATATTGTGCC
CCGGCAGAAGGATGTAGCAGTGCAACTACGTATAGGAAAAGATTTAATGACGAGACAGTGTATTGAAAAGGAGTTTGGAG
AAAAACTTGTCTCACATTTCAAATTTTTAGCATCTATGGACATAGGAGAGAAACGGAAGCCACAGAATGGTTCGTTGTAT
TTGCAAATTGATGGACAAGAAGTGTATTTACGCCTTTCAACACTTCCAACAGTATATCAAGAAAGTCTCGTTATTCGCCT
TCATTTACAAGCATCTGTTCAGCCCTTATCACATCTTTCGTTATTTCCAAGTACGGCAGCAAAACTACTCTCGTTTTTAC
GCTATTCACAGGGGCTACTCGTATTTACTGGACCGACTGGATCTGGGAAGACGACGACAATGTATGCATTATTAGAAGTA
ATTAGAAAAAAGAAAACACGTCGCATCATTACACTTGAAGATCCGGTTGAAAAAAGAAGTGACGATGTATTACAAATTCA
AATAAATGAAAAAGCGGGTCTCACATATGAAACAGGATTAAAGGCTATTTTACGTCATGATCCAGATATTATTTTAGTCG
GTGAAATCCGGGATGAGGAAACAGCAAAAATAGCTATAAGGGCAAGTTTGACAGGACATTTAGTAATGACAACGCTGCAT
ACGAATGATGCGAAAGGAGCAATCATTCGTTTCATGGATTATGGTATAACGAGGCAAGAGATTGAACAGTCTTTATTGGC
TGTAGCTGCACAGCGACTTGTCGAATTGAAGTGTCCGTTTTGCAGAGGAAAGTGCTCAACTTTATGTAAATCAATGAGGC
AAGTAAGGCAAGCAAGCATTTATGAGCTACTATATGGACATGAGTTAAAACAAGCACTTAAAGAAGCAGATGGAGAATGT
GTTACTTACAAACATGAGACTTTAGAATCGTCAATACGAAAAGGATATGCTTTAGGTTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

55.908

100

0.559