Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   PRK74_RS05985 Genome accession   NZ_CP117421
Coordinates   1159339..1161165 (+) Length   608 a.a.
NCBI ID   WP_128266876.1    Uniprot ID   -
Organism   Bacillus cereus strain 2-6A     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1154339..1166165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRK74_RS05965 (PRK74_05965) - 1154642..1155316 (-) 675 WP_000362615.1 TerC family protein -
  PRK74_RS05970 (PRK74_05970) mecA 1155662..1156345 (+) 684 WP_000350708.1 adaptor protein MecA Regulator
  PRK74_RS05975 (PRK74_05975) - 1156418..1157962 (+) 1545 WP_000799188.1 cardiolipin synthase -
  PRK74_RS05980 (PRK74_05980) - 1158043..1159287 (+) 1245 WP_000628327.1 competence protein CoiA -
  PRK74_RS05985 (PRK74_05985) pepF 1159339..1161165 (+) 1827 WP_128266876.1 oligoendopeptidase F Regulator
  PRK74_RS05990 (PRK74_05990) - 1161297..1161470 (-) 174 WP_000750355.1 hypothetical protein -
  PRK74_RS05995 (PRK74_05995) - 1161700..1162593 (-) 894 WP_000360656.1 ClpXP adapter SpxH family protein -
  PRK74_RS06000 (PRK74_06000) - 1162593..1162991 (-) 399 WP_000043386.1 hypothetical protein -
  PRK74_RS06005 (PRK74_06005) - 1163160..1163738 (-) 579 WP_000191120.1 CYTH domain-containing protein -
  PRK74_RS06010 (PRK74_06010) - 1163883..1164254 (+) 372 WP_001180004.1 hypothetical protein -
  PRK74_RS06015 (PRK74_06015) - 1164285..1164923 (+) 639 WP_001081490.1 GTP pyrophosphokinase -
  PRK74_RS06020 (PRK74_06020) - 1164942..1165739 (+) 798 WP_000673182.1 NAD kinase -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70259.67 Da        Isoelectric Point: 4.6443

>NTDB_id=784719 PRK74_RS05985 WP_128266876.1 1159339..1161165(+) (pepF) [Bacillus cereus strain 2-6A]
MFEQNKAKALPDRNEIEEASTWRLEDIFQTDAEWEKEFQAIKELLPKLTEFKGKLGDSADNLLEALQYEDEISMRLGKLY
TYAHMRYDQDTTNSVYQALNDRATNLYSQVSSSTAYIVPEILSISEDTLQTFLKENRDLSVYEHALEEITRQRPHVLSEA
EEALLAEASEVMSSSSNTFGMLNNADLKFPSIKGEDGEEIEITHGRYIQFLESDDPRVRKDAFKAVYETYGKFKNTFAST
LSGAVKRNNFNARVRKYDSARQAALSNNNIPEAVYDQLVETVNDNLHLLHRYIDIRKRALGLDELHMYDLYTPLVPEVKM
NVKYEEAQDLLLKSLNVLGDEYVDILKEAYENRWVDVYENKGKRSGAYSSGAYGTNPYILMNWHDNVNNLFTLAHEFGHS
VHSYYTRKTQPHVYGDYSIFVAEVASTCNEALLNDYLLKTTEDKKERLYLLNHYLEGFRGTVFRQTMFAEFEHIIHKKVQ
EGHAVTPDMLTEIYYDLNKKYFGDALVIDEEIGLEWSRIPHFYYNYYVYQYATGFSAATALSKQILEEGQPAVERYINEF
LKAGSSDYPIEVLKKAGVDMASPEPVKEALQVFEEKLNELEALLFEEK

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=784719 PRK74_RS05985 WP_128266876.1 1159339..1161165(+) (pepF) [Bacillus cereus strain 2-6A]
ATGTTTGAACAAAACAAAGCGAAAGCATTACCAGATCGCAACGAGATTGAAGAAGCAAGTACGTGGCGATTAGAAGATAT
TTTCCAAACGGATGCAGAATGGGAAAAAGAATTTCAAGCTATTAAAGAGCTATTGCCGAAGTTAACTGAATTTAAAGGGA
AACTTGGTGACTCTGCGGACAATTTACTTGAGGCATTGCAATATGAAGATGAAATATCAATGCGATTAGGTAAGTTATAT
ACATATGCTCATATGCGTTACGATCAAGATACAACAAACTCTGTATATCAAGCATTAAATGATCGCGCAACAAATTTATA
TTCACAAGTATCTAGTAGCACAGCGTATATTGTTCCTGAAATTTTATCGATTTCAGAAGATACACTGCAAACATTCTTGA
AGGAAAATAGAGATTTAAGTGTATATGAACATGCATTAGAAGAAATTACACGTCAACGCCCGCACGTATTATCAGAAGCT
GAGGAAGCTTTATTAGCGGAAGCGTCTGAAGTAATGAGTTCATCAAGCAATACATTCGGTATGTTGAATAACGCGGATTT
AAAATTCCCATCTATTAAAGGGGAAGACGGAGAAGAAATAGAAATTACACATGGCCGTTACATTCAGTTTTTAGAAAGTG
ATGATCCTCGTGTTCGCAAAGATGCATTTAAAGCTGTATATGAAACGTACGGAAAATTTAAAAACACATTCGCAAGTACG
TTAAGTGGAGCAGTAAAACGTAATAATTTCAATGCGCGTGTTCGTAAATATGATTCTGCTCGCCAAGCTGCATTGAGCAA
TAATAATATTCCGGAAGCAGTATACGATCAACTCGTTGAAACAGTAAATGACAACTTACATTTATTACACCGTTACATCG
ATATTCGTAAGCGTGCACTAGGACTTGATGAGCTTCATATGTATGATTTATATACACCACTTGTACCAGAAGTGAAAATG
AATGTGAAATACGAAGAAGCGCAAGACCTTTTATTAAAATCTTTAAACGTACTTGGTGATGAATATGTTGACATTTTGAA
AGAGGCATATGAAAATCGCTGGGTAGATGTGTATGAGAATAAAGGAAAACGAAGCGGGGCATATTCATCTGGTGCATATG
GAACAAATCCGTATATTTTAATGAACTGGCATGATAATGTAAATAATTTATTTACACTTGCTCATGAGTTTGGTCATTCG
GTGCATAGTTACTATACAAGAAAAACACAACCGCACGTATATGGTGATTATTCCATCTTCGTAGCAGAAGTGGCATCTAC
TTGTAATGAAGCGCTTTTAAATGATTATTTATTAAAAACGACAGAAGATAAGAAAGAGCGTCTATATTTATTAAATCATT
ATTTAGAAGGGTTCCGTGGTACTGTATTCCGTCAAACGATGTTTGCAGAGTTTGAACATATTATTCATAAGAAAGTACAA
GAAGGACATGCTGTCACGCCAGACATGCTAACGGAAATTTACTACGATTTAAATAAGAAATATTTCGGTGACGCTTTAGT
AATCGACGAAGAGATTGGTTTAGAATGGTCTCGTATTCCACACTTCTACTACAACTATTACGTATATCAATACGCAACAG
GATTTAGTGCAGCGACAGCTCTGTCTAAACAGATTTTAGAAGAAGGACAACCAGCAGTAGAACGTTATATTAACGAGTTC
TTAAAAGCAGGAAGCTCTGATTATCCAATTGAAGTGCTGAAAAAAGCAGGAGTAGATATGGCATCTCCTGAACCAGTAAA
AGAGGCGCTACAAGTATTTGAAGAGAAATTAAATGAATTAGAAGCATTATTATTTGAAGAAAAGTAA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-591)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50.084

97.533

0.488