Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   IL310_RS03140 Genome accession   NZ_CP117409
Coordinates   209564..210925 (+) Length   453 a.a.
NCBI ID   WP_201248639.1    Uniprot ID   -
Organism   Lactococcus lactis strain LB7     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 204564..215925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL310_RS03095 (IL310_03100) - 204953..205600 (+) 648 WP_010906298.1 adenylate kinase -
  IL310_RS03100 (IL310_03105) infA 205897..206115 (+) 219 WP_003130554.1 translation initiation factor IF-1 -
  IL310_RS03105 (IL310_03110) rpmJ 206169..206285 (+) 117 WP_001808836.1 50S ribosomal protein L36 -
  IL310_RS03110 (IL310_03115) rpsM 206303..206668 (+) 366 WP_003130555.1 30S ribosomal protein S13 -
  IL310_RS03115 (IL310_03120) rpsK 206689..207072 (+) 384 WP_010906297.1 30S ribosomal protein S11 -
  IL310_RS03120 (IL310_03125) - 207120..208058 (+) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  IL310_RS03125 (IL310_03130) rplQ 208077..208457 (+) 381 WP_003130558.1 50S ribosomal protein L17 -
  IL310_RS03135 (IL310_03140) - 209175..209402 (+) 228 WP_201248640.1 heavy-metal-associated domain-containing protein -
  IL310_RS03140 (IL310_03145) radA 209564..210925 (+) 1362 WP_201248639.1 DNA repair protein RadA Machinery gene
  IL310_RS03145 (IL310_03150) - 210969..211655 (-) 687 WP_012898598.1 YoaK family protein -
  IL310_RS03150 (IL310_03155) - 211886..212971 (+) 1086 WP_012898597.1 PIN/TRAM domain-containing protein -
  IL310_RS03155 (IL310_03160) - 213036..213665 (+) 630 WP_003129888.1 hypothetical protein -
  IL310_RS03160 (IL310_03165) - 213787..214149 (+) 363 WP_201248638.1 PadR family transcriptional regulator -
  IL310_RS03165 (IL310_03170) - 214136..214483 (+) 348 WP_012898595.1 DUF1048 domain-containing protein -
  IL310_RS03170 (IL310_03175) - 214470..214793 (+) 324 WP_201248637.1 DUF1048 domain-containing protein -
  IL310_RS03175 (IL310_03180) - 214885..215379 (-) 495 WP_012898594.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49863.26 Da        Isoelectric Point: 5.1748

>NTDB_id=784626 IL310_RS03140 WP_201248639.1 209564..210925(+) (radA) [Lactococcus lactis strain LB7]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTAPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=784626 IL310_RS03140 WP_201248639.1 209564..210925(+) (radA) [Lactococcus lactis strain LB7]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCCCGGATTGAAACGGATCTAGATGAATTTAATCGTGTA
TTAGGAGGGGGCGTCGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCCACTCAATTGGCTTCTAGAGGGCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTAGGAGATATTGACCGTGACTTTTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATCATGACACCAGAAATTCAATCAACTCAAGGCTCTGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACTTTTATTGTTGGTCATG
TGACTAAAGAAGGACAGCTGGCTGGTCCAAGGATGTTGGAGCACATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
ACATGGCTTGGTCGAAGTGACTAACCCCTCAGAGGTCTTTTTAGAAGAACGCTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCTATACTAGTTGAAATACAAGCACTGACGGCACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGACTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAAAAGAGAACGGGGCTCTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCGGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCGGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGTTTTAAAAAAGTATATGCTCCTAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTAACAACTTTAACTGAATGTTTAAAACTAGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.015

100

0.81
  radA Streptococcus pneumoniae D39

81.015

100

0.81
  radA Streptococcus pneumoniae R6

81.015

100

0.81
  radA Streptococcus pneumoniae TIGR4

81.015

100

0.81
  radA Streptococcus mitis SK321

81.015

100

0.81
  radA Streptococcus mitis NCTC 12261

80.795

100

0.808
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62