Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   Q2B68_RS09770 Genome accession   NZ_CP130280
Coordinates   1975479..1975913 (+) Length   144 a.a.
NCBI ID   WP_048367275.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain PM415     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1970479..1980913
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Q2B68_RS09765 (Q2B68_09765) - 1973979..1975190 (-) 1212 WP_063174873.1 cytochrome P450 -
  Q2B68_RS09770 (Q2B68_09770) nucA/comI 1975479..1975913 (+) 435 WP_048367275.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  Q2B68_RS09775 (Q2B68_09775) - 1975973..1976728 (+) 756 WP_003154084.1 YoaK family protein -
  Q2B68_RS09780 (Q2B68_09780) - 1976762..1977124 (-) 363 WP_003154082.1 hypothetical protein -
  Q2B68_RS09785 (Q2B68_09785) - 1977318..1978646 (-) 1329 WP_302565222.1 S8 family peptidase -
  Q2B68_RS09790 (Q2B68_09790) - 1978826..1979059 (+) 234 WP_031306556.1 hypothetical protein -
  Q2B68_RS09795 (Q2B68_09795) - 1979315..1980022 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  Q2B68_RS09800 (Q2B68_09800) - 1980082..1980534 (+) 453 WP_014305039.1 OsmC family protein -
  Q2B68_RS09805 (Q2B68_09805) - 1980548..1980901 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15465.41 Da        Isoelectric Point: 7.3203

>NTDB_id=781769 Q2B68_RS09770 WP_048367275.1 1975479..1975913(+) (nucA/comI) [Bacillus amyloliquefaciens strain PM415]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKAAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=781769 Q2B68_RS09770 WP_048367275.1 1975479..1975913(+) (nucA/comI) [Bacillus amyloliquefaciens strain PM415]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGCCGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTATGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

55.645

86.111

0.479