Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MWM20_RS01985 Genome accession   NZ_AP025677
Coordinates   424926..425603 (-) Length   225 a.a.
NCBI ID   WP_000178168.1    Uniprot ID   -
Organism   Escherichia coli strain Rb-23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419926..430603
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MWM20_RS01970 (TRECRb23_03870) chiA 421284..423986 (+) 2703 WP_000773178.1 bifunctional chitinase/lysozyme -
  MWM20_RS01975 (TRECRb23_03880) bfd 424155..424349 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  MWM20_RS01980 (TRECRb23_03890) bfr 424421..424897 (+) 477 WP_000675503.1 bacterioferritin -
  MWM20_RS01985 (TRECRb23_03900) pilD 424926..425603 (-) 678 WP_000178168.1 prepilin peptidase GspO Machinery gene
  MWM20_RS01990 (TRECRb23_03910) gspM 425603..426064 (-) 462 WP_001385521.1 type II secretion system protein GspM -
  MWM20_RS01995 (TRECRb23_03920) gspL 426061..427224 (-) 1164 WP_001296464.1 type II secretion system protein GspL -
  MWM20_RS02000 (TRECRb23_03930) gspK 427239..428222 (-) 984 WP_001058515.1 type II secretion system minor pseudopilin GspK -
  MWM20_RS02005 (TRECRb23_03940) gspJ 428215..428802 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  MWM20_RS02010 (TRECRb23_03950) gspI 428795..429172 (-) 378 WP_001041749.1 type II secretion system minor pseudopilin GspI -
  MWM20_RS02015 (TRECRb23_03960) gspH 429169..429678 (-) 510 WP_001076039.1 type II secretion system minor pseudopilin GspH -
  MWM20_RS02020 (TRECRb23_03970) gspG 429686..430123 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24856.72 Da        Isoelectric Point: 7.6364

>NTDB_id=78098 MWM20_RS01985 WP_000178168.1 424926..425603(-) (pilD) [Escherichia coli strain Rb-23]
MTMLLPLFILVGFIAGYFVNAMAYHLSPLEDKTALTFRQVLIHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=78098 MWM20_RS01985 WP_000178168.1 424926..425603(-) (pilD) [Escherichia coli strain Rb-23]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGATTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTCATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387

Multiple sequence alignment