Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PIB32_RS16305 Genome accession   NZ_CP116898
Coordinates   3849398..3850498 (+) Length   366 a.a.
NCBI ID   WP_308364779.1    Uniprot ID   -
Organism   Microbulbifer sp. RZ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3844398..3855498
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB32_RS16285 ggt 3844451..3846169 (-) 1719 WP_308364773.1 gamma-glutamyltransferase -
  PIB32_RS16290 coaD 3846231..3846707 (-) 477 WP_308364775.1 pantetheine-phosphate adenylyltransferase -
  PIB32_RS16295 - 3846819..3848696 (-) 1878 WP_308364777.1 fructose-specific PTS transporter subunit EIIC -
  PIB32_RS16300 rsmD 3848733..3849314 (-) 582 WP_308364778.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PIB32_RS16305 pilA 3849398..3850498 (+) 1101 WP_308364779.1 signal recognition particle-docking protein FtsY Machinery gene
  PIB32_RS16310 ftsE 3850613..3851299 (+) 687 WP_308364780.1 cell division ATP-binding protein FtsE -
  PIB32_RS16315 ftsX 3851280..3852326 (+) 1047 WP_308364782.1 permease-like cell division protein FtsX -
  PIB32_RS16320 rpoH 3852541..3853395 (+) 855 WP_308364783.1 RNA polymerase sigma factor RpoH -
  PIB32_RS16325 - 3853575..3853952 (+) 378 WP_308364784.1 DUF423 domain-containing protein -
  PIB32_RS16330 trmB 3854101..3854817 (+) 717 WP_308364785.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 39224.46 Da        Isoelectric Point: 4.8513

>NTDB_id=780976 PIB32_RS16305 WP_308364779.1 3849398..3850498(+) (pilA) [Microbulbifer sp. RZ01]
MIFDFLRKKQPQPGKTEAEEAAPESAPAEEPAAPEPRQPAESARPEAVAESAPQPAKTEGFFSRIRRGLSRTSSQFAEGM
GNLFLGAKEIDEDLLEELETHLLMADVGVEATGEIVERLTGRVSRRELADGEALYNALQGELADLLDGVEAPLEIDTAKR
PFVILVVGVNGVGKTTTIGKLAHRYLNQGRSVMLAAGDTFRAAAVEQLQVWGERHNVPVVAQHTGADSASVIYDAVQSAQ
ARGIDIVIADTAGRLHTKSNLMEELTKVRRVMGKLDESAPHEVMLVLDAGTGQNAISQAQQFRDAAGVTGLVLTKLDGTA
KGGVIFALARKFGIPVRFIGVGEQAEDLQPFKAREFVAALFAREPG

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=780976 PIB32_RS16305 WP_308364779.1 3849398..3850498(+) (pilA) [Microbulbifer sp. RZ01]
ATGATCTTTGACTTCCTGCGCAAAAAGCAGCCCCAGCCCGGCAAAACCGAGGCCGAAGAGGCAGCCCCTGAATCAGCGCC
GGCGGAGGAGCCCGCCGCGCCTGAGCCCCGACAGCCCGCCGAGTCGGCGCGGCCGGAGGCGGTTGCCGAAAGCGCACCGC
AGCCGGCGAAGACAGAGGGCTTCTTCTCCCGCATCCGCCGCGGCCTCAGCCGCACCAGCAGCCAGTTCGCCGAGGGGATG
GGCAACCTGTTCCTGGGCGCCAAGGAGATCGACGAGGATCTGCTGGAGGAACTGGAGACCCACCTGCTGATGGCGGACGT
GGGGGTGGAGGCCACCGGGGAGATTGTCGAGCGGCTTACCGGCCGGGTATCCCGCCGCGAACTGGCCGACGGCGAGGCGC
TGTACAATGCGCTGCAGGGGGAACTGGCGGACCTGCTCGACGGCGTCGAGGCGCCGCTGGAGATCGACACCGCGAAGAGA
CCTTTCGTGATCCTGGTGGTGGGCGTCAACGGTGTGGGCAAGACCACCACCATCGGCAAGCTGGCGCACCGCTATCTGAA
CCAGGGCCGCTCGGTGATGCTGGCCGCCGGCGACACCTTCCGTGCCGCGGCGGTGGAGCAGCTGCAGGTCTGGGGCGAGC
GCCACAATGTGCCGGTGGTGGCCCAGCACACCGGCGCCGACAGCGCCTCGGTCATCTACGATGCGGTGCAGTCGGCCCAG
GCCAGGGGCATCGATATCGTGATCGCCGACACCGCCGGCCGCCTGCACACCAAGTCCAACCTGATGGAGGAACTGACCAA
GGTGCGCCGGGTGATGGGCAAGCTGGACGAGAGTGCGCCCCACGAGGTGATGCTGGTGCTGGACGCTGGCACCGGCCAGA
ACGCCATCAGCCAGGCTCAGCAGTTCCGCGACGCCGCGGGCGTCACCGGGCTGGTGCTGACCAAGCTGGACGGCACCGCC
AAGGGCGGGGTGATCTTTGCGCTGGCGCGTAAGTTCGGGATCCCGGTACGCTTTATCGGCGTCGGCGAGCAGGCGGAGGA
CCTGCAGCCGTTTAAGGCGCGGGAATTCGTCGCCGCGCTGTTCGCACGTGAGCCCGGCTGA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(161-361)

SRP54-type protein, helical bundle domain (SRP54_N)

(78-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

52.478

93.716

0.492